一个西瓜叶色黄化突变体的生理特性分析Physiological characteristic analysis of a leaf-yellowing mutant in watermelon
任艺慈;朱迎春;孙德玺;邓云;安国林;李卫华;刘君璞;
摘要(Abstract):
【目的】分析西瓜叶色黄化突变体的生理特性,为叶色标记的研究和应用提供理论参考。【方法】将西瓜叶色黄化突变体(YL)与正常绿叶西瓜(ZK)的生理特征进行比较,分析其叶片超微结构,光合色素的含量,光合参数,抗氧化酶活性及丙二醛含量。【结果】西瓜叶色黄化突变体YL的叶绿体超微结构基粒片层数少,与正常绿叶西瓜ZK相比叶绿体发育不完全,YL叶片叶绿素a、叶绿素b、类胡萝卜素含量平均比ZK低80.95%、83.87%、69.87%;YL的净光合速率和气孔导度显著低于正常绿叶植株,胞间CO_2浓度以及蒸腾速率低于ZK但差异不显著。YL的抗氧化酶含量低于正常绿叶植株,而伸蔓期丙二醛含量为0.56μmol·L~(-1),显著高于ZK。【结论】光合色素的减少是导致叶色黄化的主要原因;而叶绿体发育不完全,影响植株捕光能力,致使净光合速率下降,植株代谢能力减弱。
关键词(KeyWords): 西瓜;叶色黄化突变体;叶绿体超微结构;光合特性;抗氧化酶活性;MDA含量
基金项目(Foundation): 国家现代农业产业技术体系(CARS-25);; 郑州市重大科技专项(188PCXZX802);; 中国农业科学院基本科研业务费专项(Y2018YJ15,Y2019XK16-03);中国农业科学院科技创新工程(CAAS-ASTIP-2018-ZFRI);; 国家重点研发计划(2018YFD0201310)
作者(Author): 任艺慈;朱迎春;孙德玺;邓云;安国林;李卫华;刘君璞;
Email:
DOI: 10.13925/j.cnki.gsxb.20190508
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