张雅玲;方智振;赖钟雄;

• 1:福建农林大学园艺学院园艺植物生物工程研究所
• 2:福建省农业科学院果树研究所

摘要(Abstract)：

【目的】探讨三明野生蕉Ran基因在低温胁迫响应过程中的作用。【方法】通过RT-PCR与RACE相结合的方法和q RT-PCR克隆Ran基因,并对其在响应低温胁迫和水杨酸处理过程中的表达进行分析。【结果】分离得到6条含有完整开放阅读框的Ran基因c DNA序列,编码的蛋白质与其他植物Ran蛋白高度同源,带有GTP水解结构域、Ran GAP结合结构域和酸性尾巴等典型结构域。q PCR分析结果表明,三明野生蕉Ran基因在根、叶片、花序轴、苞片、花、果皮和果肉中均有表达,在根中表达量最低,在叶片、花和果肉中的表达量较高。此外,低温胁迫和水杨酸处理可显著影响三明野生蕉Ran基因的表达。【结论】三明野生蕉Ran基因与细胞分裂、低温胁迫响应和水杨酸信号转导有关。

关键词(KeyWords)： 野生蕉;低温胁迫;Ran基因;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家香蕉产业体系专项资金(CARS-32-11);; 福建省重大农业科技平台(2008N2001)

作者(Author): 张雅玲;方智振;赖钟雄;

Email:

DOI: 10.13925/j.cnki.gsxb.20140194

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