张雅玲;方智振;赖钟雄;

• 1:福建农林大学园艺学院园艺植物生物工程研究所
• 2:福建省农业科学院果树研究所

摘要(Abstract)：

【目的】探讨三明野生蕉Ran基因在低温胁迫响应过程中的作用。【方法】通过RT-PCR与RACE相结合的方法和q RT-PCR克隆Ran基因,并对其在响应低温胁迫和水杨酸处理过程中的表达进行分析。【结果】分离得到6条含有完整开放阅读框的Ran基因c DNA序列,编码的蛋白质与其他植物Ran蛋白高度同源,带有GTP水解结构域、Ran GAP结合结构域和酸性尾巴等典型结构域。q PCR分析结果表明,三明野生蕉Ran基因在根、叶片、花序轴、苞片、花、果皮和果肉中均有表达,在根中表达量最低,在叶片、花和果肉中的表达量较高。此外,低温胁迫和水杨酸处理可显著影响三明野生蕉Ran基因的表达。【结论】三明野生蕉Ran基因与细胞分裂、低温胁迫响应和水杨酸信号转导有关。

关键词(KeyWords)： 野生蕉;低温胁迫;Ran基因;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家香蕉产业体系专项资金(CARS-32-11);; 福建省重大农业科技平台(2008N2001)

作者(Author): 张雅玲;方智振;赖钟雄;

Email:

参考文献(References)：

• [1]CLARKE P R,ZHANG C.Spatial and temporal coordination of mitosis by Ran GTPase[J].Nature Reviews Molecular Cell Biology,2008,9(6):464-477.
• [2]HE H,WANG Q,ZHENG W,WANG J,SONG Q Z.Function of nuclear transport factor 2 and Ran in the 20E signal transduction pathway in the cotton bollworm,Helicoverpa armigera[J].BMC Cell Biology,2010,11(1):1.
• [3]WANG X,XU Y,HAN Y,BAO S,DU J,MING Y,XU Z,CHONG K.Overexpression of RAN1 in rice and Arabidopsis alters primordial meristem,mitotic progress,and sensitivity to auxin[J].Plant Physiology,2006,140(1):91-101.
• [4]WANG Xin.Functional analysis of Ta RAN1,a small GTP-binding protein during cell cycle and development in plant[D].Beijing:Chinese Academy of Sciences,2004.王昕.一种小G蛋白Ta RAN1在植物细胞周期和发育过程中的功能研究[D].北京:中国科学院,2004.
• [5]LYU S,FAN Y,JIN C.Overexpression of a Ran GTPase homologous gene,Fa Ran from tall fescue,in transgenic Arabidopsis[J].Biologia Plantarum,2011,55(2):331-334.
• [6]ZANG A,XU X,NEILL S,CAI W.Overexpression of Os RAN2 in rice and Arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress[J].Journal of Experimental Botany,2010,61(3):777-789.
• [7]CHEN N,XU Y,WANG X,DU C,DU J,YUAN M,XU Z,CHONG K.Os RAN2,essential for mitosis,enhances cold tolerance in rice by promoting export of intranuclear tubulin and maintaining cell division under cold stress[J].Plant,Cell&Environment,2011,34(1):52-64.
• [8]XU C,HUANG B.Root proteomic responses to heat stress in two Agrostis grass species contrasting in heat tolerance[J].Journal of Experimental Botany,2008,59(15):4183-4194.
• [9]KAZUYA Y,AKIKO M,MASAYOSHI K,AKIHO Y,KINYA A.Programmed proteome response for drought avoidance/tolerance in the root of a C3 Xerophyte(wild watermelon)under water deficits[J].Plant and Cell Physiology,2008,49(2):226-241.
• [10]MAHONG B,ROYTRAKUL S,PHAONAKLOP N,WONGRATANA J,YOKTHONGWATTANA K.Proteomic analysis of a model unicellular green alga,Chlamydomonas reinhardtii,during shortterm exposure to irradiance stress reveals significant down regulation of several heat-shock proteins[J].Planta,2012,235(3):499-511.
• [11]YAN Yuanyuan,WANG Jingyi,FENG Suping,CHEN You,WU Yaoting.Construction and primary analysis of a SSH c DNA library of the young fruit of banana[J].Journal of Fruit Science,2013,30(3):373-380.颜园园,王静毅,冯素萍,陈友,武耀廷.香蕉幼果SSH文库构建及初步分析[J].果树学报,2013,30(3):373-380.
• [12]BAKRY F,CARREEL F,JENNY C,HORRY J P.Genetic improvement of banana[C]//JAIN S M,PRIYADARSHAN P M.Breeding Plantation Tree Crops:Tropical Species.Springer New York,2009.
• [13]CHEN Yaping,CHEN Yunfeng,HUANG Xia,XIAO Wang,HUANG Xuelin.PCR-RFLP analysis of three wild bananas and their resistance to Fusarium Wilt[J].Acta Horticulturae Sinica,2008,35(1):19-26.陈雅平,陈云凤,黄霞,肖望,黄学林.3个野生香蕉株系的PCR-RFLP分析及其对枯萎病抗性的研究[J].园艺学报,2008,35(1):19-26.
• [14]LAI Zhongxiong,CHEN Yuan,LIN Yuling,ZHAO Qiaoyang,CHEN Yiting.Discovery and taxonomy of wild banana(Musa spp.,'AA'Group)in Fuzhou[J].Subtropical Agriculture Research,2007,3(1):1-5.赖钟雄,陈源,林玉玲,赵巧阳,陈义挺.福州野生蕉(Musa spp.,AA Group)的发现及其分类学地位的初步确定[J].亚热带农业研究,2007,3(1):1-5.
• [15]LAI Zhongxiong,CHEN Yuan,LIN Yuling,ZHAO Qiaoyang,CHEN Yiting,ZHANG Zhitong.Investigation of basic biological characteristics of wild banana(Musa spp.,‘AB’Group)in Sanming City[J].Subtropical Agriculture Research,2007b,2(4):241-244.赖钟雄,陈源,林玉玲,赵巧阳,陈义挺,张知通.三明野生蕉基本生物学特性调查[J].亚热带农业研究,2007b,2(4):241-244.
• [16]ZHANG Rui.RAPD analysis,in vitro culture and cloning of SOD gene of the banana(Musa Spp.)germplasm in Fujian province[D].Fuzhou:Fujian Agriculture and Forestry University,2011.张锐.福建野生蕉资源RAPD分析,离体培养与SOD基因克隆[D].褔州:福建农林大学,2011.
• [17]LIN Y,LAI Z.Reference gene selection for q PCR analysis during somatic embryogenesis in longan tree[J].Plant Science,2010,178(4):359-365.
• [18]D’HONT A,DENOEUD F,AURY J M,BAURENS F C,CARREEL F,GARSMEUR O,NOEL B,BOCS S,DROC G,ROUARD M,SILVA C DA,JABBARI K,CARDI C,POULAIN J,SOUQUET M,LABADIE K,JOURDA C,LENGELLE J,RODIERGOUD M,ALBERTI A,BERNARD M,CORREA M,AYYAMPALAYAM S,MCKAIN M R,LEEBENS-MACK J,BURGESS D,FREELING M,MBEGUIE A MBEGUIE D,CHABANNES M,WICKER T,PANAUD O,BARBOSA J,HRIBOVA E,HESLOPHARRISON P,HABAS R,RIVALLAN R,FRANCOIS P,POIRON C,KILIAN A,BURTHIA D,JENNY C,BAKRY F,BROWN S,GUIGNON V,KEMA G,DITA M,WAALWIJK C,JOSEPH S,DIEVART A,JAILLON O,LECLERCQ J,ARGOUT X,LYONS E,ALMEIDA A,JERIDI M,DOLEZEL J,ROUX N,RISTERUCCI A M,WEISSENBACH J,RUIZ M,GLASZMANN J C,QUETIER F,YAHIAOUI N,WINCKER P.The banana(Musa acuminata)genome and the evolution of monocotyledonous plants[J].Nature,2012,488(7410):213-217.
• [19]TAKAI Y,SASAK T,MATOZAKI T.Small GTP-binding proteins[J].Physiological Reviews,2001,81(1):153-208.
• [20]MA Q H.Small GTP-binding proteins and their functions in plants[J].Journal of Plant Growth Regulation,2007,26(4):369-388.
• [21]MERKLE T.Nucleo-cytoplasmic partitioning of proteins in plants:implications for the regulation of environmental and developmental signalling[J].Current Genetics,2003,44(5):231-260.
• [22]IRIS M,DAVID E S.Regulation of nucleocytoplasmic trafficking in plants[J].Current Opinion in Plant Biology,2011,14(5):538-546.
• [23]CECILIA C C,PALOMAR M,MARIO A V,REYES J,ALEJANDRAA C.Non-coding RNAs in the plant response to abiotic stress[J].Planta,2012,236(4):943-958.
• [24]QIAO X,PHAM D,LUO H,WU J.Ran overexpression leads to diminished t cell responses and selectively modulates nuclear levels of c-Jun and c-Fos[J].Journal of Biological Chemistry,2010,285(8):5488-5496.
• [25]XIA F,LEE C W,ALTIERI D C.Tumor cell dependence on RanGTP–directed mitosis[J].Cancer Research,2008,68(6):1826-1833.
• [26]ABE H,KAMAI T,SHIRATAKI H,OYAMA T,ARAI K,YOSHIDA K I.High expression of Ran GTPase is associated with local invasion and metastasis of human clear cell renal cell carcinoma[J].International Journal of Cancer,2008,122(10):2391-2397.
• [27]HAIZEL T,MERKLE T,PAY A,FEJES E,NAGY F.Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis[J].The Plant Journal,1997,11(1):93-103.
• [28]HE Lihong,CHEN Jianye,WANG Hailan,KUANG Jianfei,LU Wangjin.Cloning and expression analysis of two fragments of HSP70 c DNA in the peel of banana during storage at high and low temperature[J].Journal of Fruit Science,2012,29(2):241-245.贺立红,陈建业,王海蓝,邝健飞,陆旺金.香蕉果皮2个HSP70基因片段的克隆及其在热激和冷藏过程中的表达[J].果树学报,2012,29(2):241-245.
• [29]LIU Kai,HU Chunhua,DU Faxiu,ZHANG Yu’e,WEI Yuerong,YI Ganjun.Over-expression of the arabidopsis CBF1 gene in Dongguandajiao(Musa spp.ABB group)and detection of its cold resistance[J].Scientia Agricultura Sinica,2012,45(008):1653-1660.刘凯,胡春华,杜发秀,张玉娥,魏岳荣,易干军.东莞大蕉超表达拟南芥CBF1基因及其抗寒性检测[J].中国农业科学,2012,45(008):1653-1660.
• [30]LIN Guimei,LI Xiaoquan,WEI Shaolong,ZOU Yu,MOU Haifei,LI Chaosheng,WEI Huafang,ZHANG Jinzhong.Investigation on chilling affected banana plantations during early spring season and restoration strategies[J].Journal of Southern Agriculture,2012,43(1):46-49.林贵美,李小泉,韦绍龙,邹瑜,牟海飞,李朝生,韦华芳,张进忠.2011年早春我国香蕉寒害调查及寒害后恢复对策[J].南方农业学报,2012,43(1):46-49.
• [31]PAUL A,KUMAR S.Responses to winter dormancy,temperature,and plant hormones share gene networks[J].Functional&Integrative Genomics,2011,11(4):659-664.
• [32]MUTLU S,KARADAGOGLU,ATICI O,NALBANTOGLU B.Protective role of salicylic acid applied before cold stress on antioxidative system and protein patterns in barley apoplast[J].Biologia Plantarum,2013,57(3):507-513.
• [33]MARTíNEZ C,PONS E,PRATS G,LEóN J.Salicylic acid regulates flowering time and links defence responses and reproductive development[J].The Plant Journal,2004,37(2):209-217.
• [34]KANG G,WANG C,SUN G,WANG Z.Salicylic acid changes activities of H2O2-metabolizing enzymes and increases the chilling tolerance of banana seedlings[J].Environmental and Experimental Botany,2003,50(1):9-15.