张志红;冯宏;肖相政;李华兴;

• 1:华南农业大学资源与环境学院

摘要(Abstract)：

通过盆栽试验比较堆肥、生物有机肥和生物复混肥3种肥料灭菌前后对粉蕉(Musa paradisica,AAB)促生和枯萎病防病效果,采用Biolog Eco微平板方法研究不同肥料对土壤微生物功能多样性影响以及与防病关系。结果表明,所有施肥处理病情指数均比单接病原菌低,生物有机肥和生物复混肥防病效果分别为61.5%和53.8%,同相应肥料灭菌处理和堆肥比较,功能细菌在防病效果中具有重要作用。生物有机肥和生物复混肥明显促进粉蕉生长,鲜质量分别比单接病原菌高35.0%和70.9%。Biolog结果表明,单接病原菌处理AWCD和Shannon diversity指数比空白对照高,施肥处理又不同程度低于单接病原菌,Shannon eveness指数差异不明显。肥料防病效果与土壤微生物功能多样性变化趋势相反。

关键词(KeyWords)： 香蕉;枯萎病;生物肥;防病效果;土壤微生物多样性;Biolog

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(40971155);; 广东省教育部产学研结合项目(2009B090300330)

作者(Author): 张志红;冯宏;肖相政;李华兴;

Email:

DOI: 10.13925/j.cnki.gsxb.2010.04.019

参考文献(References)：

• [1]ZENG Xi-bing,WANG Bi-qing,HAN Lu,HUANG Bing-zhi,YANG Hu.Identification of resistance of banana resource to Fusari-um wilt disease[J].China Fruits,1996(2):28-29.曾惜冰,王碧青,韩路,黄秉智,杨护.香蕉品种资源抗枯萎病的鉴定[J].中国果树,1996(2):28-29.
• [2]THANDAVELU A R,PANIS W A,DORMS W S.The effect of Pseudonnonas fluorescens and Fusariumoxyaporum f.sp.cubense on induction of defence enzymes and phenolics in banana[J].Bio Plan-tarum,2003,46(1):107-110.
• [3]GETHA K,VIKINESWARY S,WONG W H,SEKI T,WARD A,GOODFELLOW M.Evaluation of streptomyces spp.strain g10for suppression of fusarium wilt and rhizosphere colonization in pot-grown banana plantlets[J].Journal of Industrial Microbiology&Biotechnol-ogy,2005,32:24-32.
• [4]SATHEESH S,VENU D V.Effect of various amendments and Tri-choderma viride on the fusarial wilt of banana[J].Mysore Journal of Agricultural Sciences,2004,38:546-550.
• [5]SARAVANAN T,MUTHUSAMY M,MARIMUTHU T.Development of integrated approach to manage the fusarial wilt of banana[J].Crop Protection,2003,22:1117-1123.
• [6]GARLAND J J,MILLS A L.Classification and characterization of samples of microbial communities using patterns of community-level sole-carbon-source utilization[J].Applied and Environmental Mi-crobiology,1991,57:2351-2359.
• [7]LI Bin,XIE Guan-lin,CHEN Ruo-xia,HUANG-FU Wei-guo,LIU Bo.Effects of cropping system and cultivation pattern on bacte-rial populations and anti-fusarium wilt bacteria in melon soils[J].Cinese Journal of Applied Ecology,2006,17(10):1937-1940.李斌,谢关林,陈若霞,皇甫伟国,刘波.耕作与栽培方式对瓜类土壤细菌数量及枯萎病拮抗细菌分布的影响[J].应用生态学报,2006,17(10):1937-1940.
• [8]LI Sheng-hua,GU Li-ping,LIU Ke-xing.Effects of combined ap-plication of organic fertilizers on the control of soilborne diseases and the regulation of soil microbial diversity[J].Plant Nutrition and Fer-tilizer Science,2009,15(4):965-969.李胜华,谷丽萍,刘可星.有机肥配施对番茄土传病害的防治及土壤微生物多样性的调控[J].植物营养与肥料学报,2009,15(4):965-969.
• [9]WEI Xiang-hua,LI Hua-xing,FENG Hong,ZHANG Zhi-hong,CAI Yan-fei,CHEN Yun-yun.Effect of post-havest soil after ap-plying different fertilizers on soil microoganisms and maize growth[J].Journal of Soil and Water Conservation,2009,23(2):138-141.韦翔华,李华兴,冯宏,张志红,蔡燕飞,陈昀云.不同肥料对后茬土壤微生物以及玉米生长的影响[J].水土保持学报,2009,23(2):138-141.
• [10]ZHAO Lan-feng,LI Hua-xing,GOU Wu-long,ZHANG Li-juan,HU Wei,LI Yong-tao.Effect of doses of compound biofertilizer on soil nutritions and crop growth[J].Soils,2009,41(2):248-252.赵兰凤,李华兴,缑武龙,张丽娟,胡伟,李永涛.生物复混肥施用量对土壤养分以及作物生长的影响[J].土壤,2009,41(2):248-252.
• [11]ZHANG Zhi-hong,LI Hua-xing,WEI Xiang-hua,LIU Xu,PENG Gui-xiang.Infulence of biological fertilizers on banana wilt disease and microorganisms in soil[J].Ecology and Environment,2008,17(6):2421-2425.张志红,李华兴,韦翔华,刘序,彭桂香.生物肥料对香蕉枯萎病及土壤微生物的影响[J].生态环境,2008,17(6):2421-2425.
• [12]XU Zhi-gang.General Plant Pathology[M].Beijing:China Agricul-tural Press,2002:236-262.许志刚.普通植物病理学[M].北京:中国农业出版社,2002:236-262.
• [13]XU Qiu-fang,JIANG Pei-kun,XU Zhi-hong.Soil microbial func-tional diversity under intensively managed bamboo plantations in southern China[J].Journal of Soils and Sediments,2008,8:177-183.
• [14]TAM L,DERRY A M,KEVAN P G.Functional diversity and com-munity structure of microorganisms in rhizosphere and non-rhizo-sphere Canadian arctic soils[J].Biodiversity and Conservation,2001,10:1933-1947.
• [15]XU Wen-yao,WU Xu-hui,YANG Jing-hui,YANG Kun.The pathogenic reaction of banana pesudostem cells to different races of vascular wilt fungus and their crude toxins[J].Acta Phytopathologica Sinica,2004,34(5):425-430.许文耀,兀旭辉,杨静惠,杨琨.香蕉假茎细胞对枯萎病菌不同小种及其粗毒素的病理反应[J].植物病理学报,2004,34(5):425-430.
• [16]SCHUTTER M E,DICK R P.Shifts in substrate utilization potential and structure of soil microbial communities in response to carbon substrates[J].Soil Biology and Biochemistry,2001,33:1481-1491.
• [17]TAN Zhao-zan,XU Guang-mei,LIU Ke-xing,LIAO Zong-wen.Effects of different composts on control of tomato bacterial wilt and soil microbial functional diversity[J].Journal of South China Agricul-tural University,2009,30(2):10-13.谭兆赞,徐广美,刘可星,廖宗文.不同堆肥对番茄青枯病的防病效果及土壤微生物群落功能多样性的影响[J].华南农业大学学报,2009,30(2):10-13.
• [18]ZHAO Na,CAI Kun-zheng,WANG Guo-ping,WANG Yong.In-duced resistance of tomato plants to bacterial wilt by livestock wastes compost and its physiological mechanisms[J].Journal of Agro-Envi-ronment Science,2008,27(5):2058-2063.赵娜,蔡昆争,汪国平,王勇.家畜堆肥诱导番茄对青枯病的抗性及其生理机制[J].农业环境科学学报,2008,27(5):2058-2063.
• [19]ZHANG S S,RAZA W,YANG X M,HU J,HUANG Q W,XU Y C,LIU X H,RAN W,SHEN Q R.Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer[J].Biology and Fertility of Soils,2008,44:1073-1080.
• [20]COTXARRERA L,TYILLAS-GAY M I,STEINBERG C,ALABOU-VETTE C.Use of sewage sludge compost and Trichoderma asperel-lum isolates to suppress fusarium wilt of tomato[J].Soil Biology&Biochemistry,2002,34:467-476.
• [21]PRESTON J M,BODDY L,RANDERSON P F.Analysis of micro-bial community functional diversity using sole-carbon source utili-sation profiles[J].Fems Microbiology Ecology,2002,42:1-14.
• [22]RAPHAEL C,KARINE L,SYLVIE B.Analysis of the potential functional diversity of the bacterial community in soil:a reproducible procedure using sole-carbon-source utilization profiles[J].European Journal of Soil Biology,2005,41:11-20.
• [23]ZHANG Yan-yan,QU Lai-ye,CHEN Li-ding.An amendment on information extraction of biolog EcoPlateTM[J].Microbiology,2009,36(7):1083-1091.张燕燕,曲来叶,陈利顶.Biolog EcoPlateTM实验信息提取方法改进[J].微生物学通报,2009,36(7):1083-1091.