李文剑;曹后男;宗成文;金灿;刘淼;

• 1:延边大学农学院

摘要(Abstract)：

【目的】为了给梨转基因研究提供稳定、高效的离体再生体系,【方法】以‘苹果梨’为试材,NN69作为基本培养基,研究了植物生长调节剂种类、暗培养时间及外植体类型等因素对其再生体系建立的影响,筛选出较适合‘苹果梨’不定芽分化的培养条件。【结果】结果表明,较适合‘苹果梨’分化的外植体为继代4次以上(含4次)的组培苗叶片,适宜分化的培养基为NN69+1.5 mg.L-1TDZ+0.4 mg.L-1NAA,结合20 d的暗培养,再生频率达到71.00%,平均再生芽数为2.21。【结论】影响‘苹果梨’离体叶片再生的最关键因素是外植体的继代次数。

关键词(KeyWords)： ‘苹果梨’;再生;不定芽

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30960231)

作者(Authors): 李文剑;曹后男;宗成文;金灿;刘淼;

DOI: 10.13925/j.cnki.gsxb.2012.05.018

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