胡钟东;乔玉山;王三红;姚泉洪;章镇;

• 1:南京农业大学园艺学院
• 2:南京农业大学园艺学院
• 3:南京农业大学园艺学院
• 4:上海市农业科学院生物技术研究中心

摘要(Abstract)：

以若光梨成熟果实为材料,提取总RNA,在ACC氧化酶基因(ACO)cDNA序列同源性较高区域设计一对特异引物,利用RT-PCR克隆了ACC氧化酶(ACO)基因片段。将ACO反义基因、与副球菌中类胡罗卜素合成有关的间隔基因YYT和ACO正义基因3个片段串联在一起,经鉴定后,插入到植物表达载体中,构建成能够表达ACC氧化酶基因的双链RNA的植物载体pYF028,为耐贮砂梨的遗传转化创造条件。

关键词(KeyWords)： 梨;ACC氧化酶(ACO);RT-PCR;克隆;RNAi载体构建

Abstract:

Keywords:

基金项目(Foundation): 江苏省高技术研究项目(BG2006313);; 南京农业大学青年科技创新基金项目(KJ05007)。

作者(Author): 胡钟东;乔玉山;王三红;姚泉洪;章镇;

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DOI:

参考文献(References)：

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