薛华柏;王芳芳;王磊;杨健;王龙;王苏珂;苏艳丽;乔玉山;李秀根;

• 1:中国农业科学院郑州果树研究所
• 2:南京农业大学园艺学院

摘要(Abstract)：

【目的】对授粉时未做去雄处理的‘满天红’ב红香酥’组合进行杂种鉴定和遗传变异分析,排除非双亲杂种单株,为利用该组合进行连锁分析奠定基础。【方法】用9对从转录组数据开发的在双亲间表现多态性的genic-SSR引物和1对自交不亲和基因检测引物对‘满天红’ב红香酥’组合所有单株进行杂种鉴定、标记偏分离分析和群体聚类分析。【结果】348个杂交单株中,有339株同时拥有母本和父本来源的特征片段,真正为‘满天红’和‘红香酥’的杂种,其余9株在部分位点未见父本条带,排除为双亲杂种的可能。卡方检验发现6个位点符合孟德尔分离定律,4个出现偏分离现象。双亲和339个真正杂种后代经遗传聚类分析分为3组:一组包含77个后代,与‘满天红’聚在一起;另一组包含183个后代,与‘红香酥’聚到一起。另有79个后代,单独聚在一起。【结论】利用Genic-SSR分析成功地对‘满天红’ב红香酥’组合梨杂种后代进行了鉴定,表明外来花粉污染是造成杂交群体中非双亲杂种污染的主要原因,产生自交后代污染的概率较低,聚类分析表明S-RNAse和部分SSR位点在该杂交群体中发生偏分离,半数以上的单株与父本‘红香酥’聚为一类。

关键词(KeyWords)： 梨;杂种鉴定;Genic-SSR;自交不亲和;偏分离

Abstract:

Keywords:

基金项目(Foundation): 中国农业科学院创新工程(ASTIP);; 现代农业(梨)产业技术体系(CARS-29);; 中央级科研院所基本科研业务费专项(1610192017709);; 河南省科技攻关项目(172102110244)

作者(Author): 薛华柏;王芳芳;王磊;杨健;王龙;王苏珂;苏艳丽;乔玉山;李秀根;

Email:

DOI: 10.13925/j.cnki.gsxb.20160244

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