殷智婷;韩剑;周国辉;张祥林;罗明;潘亚南;

• 1:新疆农业大学农学院
• 2:华南农业大学资源与环境学院
• 3:新疆出入境检疫检验局

摘要(Abstract)：

【目的】为了查明新疆巴旦木果树病原病毒的种类,为病毒的分子检测提供基础。【方法】采用双抗体夹心酶联免疫吸附法(DAS-ELISA),从巴旦木果树叶片中检测到李属坏死环斑病毒(PNRSV)。以阳性样品的总RNA为模板进行PNRSV外壳蛋白基因(CP)RT-PCR扩增,对预期430 bp大小的4个扩增产物进行克隆、测序和序列分析。【结果】结果表明,PNRSV新疆巴旦木分离物CP基因的核苷酸和氨基酸序列与世界各地已报道的分离物具有较高的同源性,分别为80.0%～97.2%和80.1%～94.1%,其中与阿根廷分离物AY217的同源性最高;而与同属03亚组的苹果花叶病毒(ApMV)同源性较低,仅为52.9%～55.4%和45.6%～48.6%。新疆PNRSV各分离物之间CP基因高度同源。序列比对与系统发生树的分析显示,PNRSV新疆巴旦木分离物的株系属于GroupⅠ组。【结论】确定了PNRSV为侵染新疆巴旦木果树的病原病毒。这是PNRSV在新疆发现的首次报道。

关键词(KeyWords)： 巴旦木;李属坏死环斑病毒(PNRSV);双抗体夹心酶联免疫吸附法(DAS-ELISA);RT-PCR;CP基因

Abstract:

Keywords:

基金项目(Foundation): 新疆自治区自然科学基金(2012211B25);; 新疆自治区科技支疆项目(200991238)

作者(Authors): 殷智婷;韩剑;周国辉;张祥林;罗明;潘亚南;

DOI: 10.13925/j.cnki.gsxb.2012.05.003

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