石艳;张剑侠;王跃进;唐冬梅;张朝红;

• 1:西北农林科技大学园艺学院
• 2:农业部西北地区园艺作物生物学与种质创制重点实验室
• 3:旱区作物逆境生物学国家重点实验室·西北农林科技大学

摘要(Abstract)：

为培育三倍体无核葡萄新品种,以二倍体欧洲葡萄无核品种红宝石无核、爱莫无核、黎明无核和欧山杂种北醇及四倍体欧美杂种藤稔、黑奥林为亲本,进行了红宝石无核×藤稔、黎明无核×藤稔、爱莫无核×黑奥林、藤稔×北醇、黑奥林×北醇5个组合的杂交,通过胚挽救技术获得杂种幼苗41株。在此基础上,利用流式细胞术、染色体计数的方法对所获得的杂种幼苗进行了早期倍性鉴定,结果表明有10株杂种为三倍体,6株为四倍体,4株为非整倍体,其余21株为二倍体。这将为进一步选育出三倍体无核葡萄新品种和提供新的育种材料奠定基础。

关键词(KeyWords)： 葡萄;多倍体;胚挽救;流式细胞术;染色体计数;育种

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2006BAD01A1702,2007BAD36B08);; 国家现代农业产业技术体系建设专项基金(CARS-30-yz-7)

作者(Author): 石艳;张剑侠;王跃进;唐冬梅;张朝红;

Email:

DOI: 10.13925/j.cnki.gsxb.2012.02.021

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