乙酰水杨酸调控猕猴桃果实后熟软化进程中的Ad-EXP1基因表达Expression pattern and regulation of Ad-EXP1 gene in kiwifruit treated with ASA
杨绍兰;陈妙金;张波;李鲜;徐昌杰;陈昆松;
摘要(Abstract):
以布鲁诺猕猴桃(Actinidia deliciosa cv.Bruno)成熟果实为材料,根据植物α-expansin(EXP)基因的氨基酸保守序列设计简并引物,利用RT-PCR方法结合3’RACE扩增得到1个长为957bp的cDNA片段(Ad-EXP1)。该基因片段编码211个氨基酸,与其它植物该基因核苷酸同源性为59%~85%,氨基酸同源性为73%~91%。Northern杂交结果表明,Ad-EXP1基因在采收当天的果实中表达很弱,随着果实成熟衰老进程加快趋于增强;乙酰水杨酸(ASA)处理延缓果实后熟软化和乙烯生成,也显著抑制Ad-EXP1基因表达。鉴于Ad-EXP1表达丰度与乙烯生成、果实软化程度的一致性,推测该基因在猕猴桃果实后熟软化进程中起着重要作用。
关键词(KeyWords): 猕猴桃;EXP(α-expansin);果实成熟;乙烯;软化;ASA
基金项目(Foundation): 国家自然科学基金(30571284);; 高等学校博士学科点专项科研基金(20040335022)
作者(Author): 杨绍兰;陈妙金;张波;李鲜;徐昌杰;陈昆松;
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参考文献(References):
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