杨绍兰;陈妙金;张波;李鲜;徐昌杰;陈昆松;

• 1:浙江大学果实分子生理与生物技术实验室农业部园艺植物生长发育与生物技术重点开放实验室
• 2:浙江大学果实分子生理与生物技术实验室农业部园艺植物生长发育与生物技术重点开放实验室
• 3:浙江大学果实分子生理与生物技术实验室农业部园艺植物生长发育与生物技术重点开放实验室
• 4:浙江大学果实分子生理与生物技术实验室农业部园艺植物生长发育与生物技术重点开放实验室

摘要(Abstract)：

以布鲁诺猕猴桃(Actinidia deliciosa cv.Bruno)成熟果实为材料,根据植物α-expansin(EXP)基因的氨基酸保守序列设计简并引物,利用RT-PCR方法结合3’RACE扩增得到1个长为957bp的cDNA片段(Ad-EXP1)。该基因片段编码211个氨基酸,与其它植物该基因核苷酸同源性为59%～85%,氨基酸同源性为73%～91%。Northern杂交结果表明,Ad-EXP1基因在采收当天的果实中表达很弱,随着果实成熟衰老进程加快趋于增强;乙酰水杨酸(ASA)处理延缓果实后熟软化和乙烯生成,也显著抑制Ad-EXP1基因表达。鉴于Ad-EXP1表达丰度与乙烯生成、果实软化程度的一致性,推测该基因在猕猴桃果实后熟软化进程中起着重要作用。

关键词(KeyWords)： 猕猴桃;EXP(α-expansin);果实成熟;乙烯;软化;ASA

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30571284);; 高等学校博士学科点专项科研基金(20040335022)

作者(Author): 杨绍兰;陈妙金;张波;李鲜;徐昌杰;陈昆松;

Email:

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