利用rep-PCR技术研究我国9省柑橘溃疡病菌遗传多样性Primary analysis on genomic diversities of Xanthomonas axnopodis pv. citri in nine provinces of China
姚廷山;胡军华;唐科志;冉春;李中安;周常勇;
摘要(Abstract):
采用rep-PCR技术,对从我国9省(区)收集、分离、筛选的18株柑橘溃疡病菌株进行遗传多样性研究,并与A菌系标准菌株进行UPGMA聚类分析和相似性分析。结果表明,使用引物BOXA1R扩增供试菌株,可得到不同的条带31条。以遗传相似距离0.4划分,供试菌株可划分为5个簇;以遗传相似距离0.5划分,供试菌株可划分为4个簇;15个菌株与标准A菌株遗传相似距离较近,菌株CJX、CZJ1、CGDN2与标准A菌株遗传相似距离较远;来自同一省的少数溃疡病菌株的遗传相似性也较低,说明我国柑橘溃疡病菌基因组存在丰富的遗传多样性。
关键词(KeyWords): 柑橘溃疡病菌;rep-PCR;遗传多样性
基金项目(Foundation): 国家科技支撑计划(2007BAD47B03);; 教育部西南大学项目(XDJK2009C142);; 重庆市重大科技专项(CSTC,2007AA1024);; 行业公益专项(nyhyzx07-023)
作者(Author): 姚廷山;胡军华;唐科志;冉春;李中安;周常勇;
Email:
DOI: 10.13925/j.cnki.gsxb.2010.05.030
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