冯海霞;郭尚敬;孟庆杰;黄勇;李妹芳;李岩岩;王光全;

• 1:聊城大学生命科学学院
• 2:甘肃农业大学生命科学与技术学院

摘要(Abstract)：

为探讨山楂品种间的亲缘关系,采用RAPD技术对20个不同品种的山楂材料进行了基因组DNA多态性分析。从120个引物中筛选出15个10bp的随机引物对所选山楂品种的DNA样品进行PCR扩增,共得到216条谱带,177条表现多态性,多态性比率达81.9%,其中包含27条特异性谱带,揭示了山楂植物丰富的遗传多样性。且利用NTSYS软件和UPGMA法对扩增结果进行了品种间相似系数的计算及聚类分析,结果表明相似系数在0.71～0.87,实生楂与其他山楂品种亲缘关系较远。

关键词(KeyWords)： 山楂;亲缘关系;RAPD

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30671242);; 山东省教育厅资助项目(J07YF17)

作者(Author): 冯海霞;郭尚敬;孟庆杰;黄勇;李妹芳;李岩岩;王光全;

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