董晓莉;汤浩茹;丁建;李明章;郑晓琴;杨足君;

• 1:四川农业大学林学园艺学院
• 2:四川农业大学林学园艺学院
• 3:四川省自然资源研究所
• 4:四川省自然资源研究所

摘要(Abstract)：

彩色猕猴桃(Actinidia deliciosa var．coloris)为美味猕猴桃(A．deliciosa var．deliciosa)的红心变种,数量稀少,十分珍贵,是中国特有的种质资源,具有重要的育种价值。采用RAPD技术研究彩色猕猴桃红美,TJ-DD—19,TJ- DD-54,TJ—DD-75和Ckou—DD-18的遗传多样性,以及它们与3个有代表性美味猕猴桃品种川猕1号,秦美和海沃德的遗传差异性。从120个引物中筛选出扩增效果较好的18个引物,共扩增出242条带,其中多态性带206条,占85．1％。各引物扩增的条带数在5～18条不等,平均每个引物扩增出的带数为13．4条。结果表明,彩色猕猴桃和美味猕猴桃具有复杂的遗传关系,它们除了变种间具有较大的遗传差异外,变种内也存在一定的遗传差异。同时,采用UPGMA法聚类的结果与传统的形态分类存在一定的差异,但部分反映了猕猴桃的地理分布。

关键词(KeyWords)： 彩色猕猴桃;美味猕猴桃;红果肉;遗传差异;RAPD(Random amplification of polymorphic DNA)

Abstract:

Keywords:

基金项目(Foundation): 教育部“新世纪优秀人才支持计划”(NCET-04—0905);; 高等学校全国优秀博士学位论文作者专项基金(200253);; 四川省科技厅攻关资助项目(04001—022-01)和(04FB013-022)

作者(Author): 董晓莉;汤浩茹;丁建;李明章;郑晓琴;杨足君;

Email:

DOI: 10.13925/j.cnki.gsxb.2006.05.004

参考文献(References)：

• [1] XU X B, ZHANG Q M(徐小彪,张秋明). Researches and utilizations of germplasm resource of kiwifruit in China[J]. Chinese Bulletin of Botany(植物学通报),2003, 20(6):648-665. (in Chinese)
• [2] LINTH, XIONG X Y(林太宏,熊兴耀). A new variety of Actinidia deliciosa[J]. Guihaia(广西植物), 1991, 11 (2):117. (in Chinese)
• [3] LIN T H, XIONG X Y(林太宏,熊兴耀). A preliminary study on the geographical distribution, the botanical characters and the main growth habits of Actinidia deliciosa var. coloris[J]. Guangdong Agricultural Sciences(广东农业科学), 1991(2): 17-19. (in Chinese)
• [4] WANG M Z, LI X D, YU Z S,et al(王明忠,李兴德,余中树,等). The selection of Actinidia deliciosa var. coloris cultivar Hongmei[J]. China Fruits(中国果树), 2005(4):7-9. (in Chinese)
• [5] ZHU D X(朱道圩). Genetics and breeding of Actinidia- a literature review [J]. Journal of Henan Agricultural University(河南农业大学学报), 1995,29(4): 328-336. (in Chinese)
• [6] CHEN X L(陈晓玲). Studies on genetic diversity of Actinidia in Heping country of Guangdong province(广东省和平县猕猴桃遗传多样性研究)[D]. Hunan: Hunan Agricultural University, 2004. (in Chinese)
• [7] JIN W L, WEN Z X, PU S J, et al(金文林,文自翔,濮绍京,等). Genetic diversity and evolution of Adzuki Bean(Vigna angularis) germplasm resources based on RAPD markers[J]. Scientia Agricul-tura Sinica(中国农业科学),2005,38(2): 241-249. (in Chinese)
• [8] HE X L, TENG H R(贺学礼,滕华容). Application of molecular markers RAPD in angiosperm classification[J]. Journal of Hebei U-niversity (Natural Science Edition) (河北大学学报:自然科学版), 2004,24(5):556-560. (in Chinese)
• [9]CHENYH, LIHT, ZHUDX, et al(陈延惠,李洪涛,朱道圩,等). Application of RAPD molecular marker on identification of germplasm resources in A ctinidia. Journal of Henan Agricultural U-niversity(河南农业大学学报),2003,37(4): 360-364. (in Chinese)
• [10] GILL G P, HARVEY C F, GARDNER R C, et al. Development of sex -linked PCR markers for gender identification in A ctinidia [J]. Theoretical and Applied Genetic, 1998, 97:439-445.
• [11] CHAT J, DUMOULIN P Y. Flow cytometry and RAPD markers applied to parentage analysis within Actinidia genus[J]. Acta Horticul-turae, 1997,444(1):109-111
• [12]DONGXL, TANGHR, CAN L,et al(董晓莉,汤浩茹,甘玲,等). Advances in research on application of DNA molecular markers in Actinidia[J]. Journal of Fruit Science(果树学报), 2005,22(6): 682-686. (in Chinese)
• [13] HUANG H W, GONG J J, WANG S M ,et al(黄宏文,龚俊杰,王圣梅,等). Genetic diversity in the genus Actinidia[J]. Biodiversity Science (生物多样性), 2000,8(1): 1-12. (in Chinese)
• [14] LI Z Z,LI L L,HUANG H W, et al(李作洲,李林林,黄宏文,等). Diversity and specific relationship in the genus Actinidia based on RAPDs and PCR-RFLPs of mtDNA [C]// Huang H W ed. Advances in Actinidia research( II) (猕猴桃研究进展(II)).Beijing: Science Press, 2003:245-261. (in Chinese)
• [15] LIANG C F(梁畴芬). On the distribution of Actinidia[J]. Guihaia(广西植物), 1983,3(4): 229-248. (in Chinese)
• [16]NING YY,XIONG QE,ZENG W G(宁允叶,熊庆娥,曾伟光). Studies on fruit quality and pollen morphology of red flesh sport from Red Sun kiwifruit[J]. Acta Horticulturae Sinica(园艺学报), 2005,32 (3): 486-488. (in Chinese)
• [17] BAO M Z(包满珠). Cloning and applications of plant anthocyanin genes[J]. Acta Horticulturae Sinica(园艺学报), 1997, 24(3): 279 -284. (in Chinese)
• [18]NINGYY, XIONG Q E, ZENGWG, et al(宁允叶,熊庆娥,曾伟光,等). Studies on red flesh sport from 'Red Sun' kiwifruit using RAPD marker[J]. Acta Horticulturae Sinica(园艺学报), 2003,30(5): 511-513. (in Chinese)
• [19]LI M Z, WANG L(李明章,王林). The advantages of Sichuan Actinidia resources in the great west-China development[J]. Resource Development&Market(资源开发与市场),2000,16 (6): 360-361. (in Chinese)