董晓莉;汤浩茹;丁建;李明章;郑晓琴;杨足君;

• 1:四川农业大学林学园艺学院
• 2:四川农业大学林学园艺学院
• 3:四川省自然资源研究所
• 4:四川省自然资源研究所

摘要(Abstract)：

彩色猕猴桃(Actinidia deliciosa var．coloris)为美味猕猴桃(A．deliciosa var．deliciosa)的红心变种,数量稀少,十分珍贵,是中国特有的种质资源,具有重要的育种价值。采用RAPD技术研究彩色猕猴桃红美,TJ-DD—19,TJ- DD-54,TJ—DD-75和Ckou—DD-18的遗传多样性,以及它们与3个有代表性美味猕猴桃品种川猕1号,秦美和海沃德的遗传差异性。从120个引物中筛选出扩增效果较好的18个引物,共扩增出242条带,其中多态性带206条,占85．1％。各引物扩增的条带数在5～18条不等,平均每个引物扩增出的带数为13．4条。结果表明,彩色猕猴桃和美味猕猴桃具有复杂的遗传关系,它们除了变种间具有较大的遗传差异外,变种内也存在一定的遗传差异。同时,采用UPGMA法聚类的结果与传统的形态分类存在一定的差异,但部分反映了猕猴桃的地理分布。

关键词(KeyWords)： 彩色猕猴桃;美味猕猴桃;红果肉;遗传差异;RAPD(Random amplification of polymorphic DNA)

Abstract:

Keywords:

基金项目(Foundation): 教育部“新世纪优秀人才支持计划”(NCET-04—0905);; 高等学校全国优秀博士学位论文作者专项基金(200253);; 四川省科技厅攻关资助项目(04001—022-01)和(04FB013-022)

作者(Author): 董晓莉;汤浩茹;丁建;李明章;郑晓琴;杨足君;

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DOI:

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