张亮;李疆;帕提曼·阿布都热合曼;罗淑萍;田嘉;王敏;

• 1:新疆农业大学农学院
• 2:新疆农业大学林学与园艺学院

摘要(Abstract)：

【目的】分离和克隆新疆栽培扁桃CBF1转录因子基因,了解其在扁桃响应环境胁迫和逆境相关激素诱导的表达情况。【方法】以新疆扁桃幼苗为试验材料,对其进行低温、干旱、盐及ABA处理,使用PCR技术从新疆扁桃‘双果’克隆得到CBF1转录因子基因,通过农杆菌将35S-Ac CBF1-GFP融合质粒转化洋葱表皮细胞后在激光共聚焦显微镜下观察结果,并通过实时荧光定量PCR技术分析了SG-Ac CBF1转录因子基因在不同非生物逆境胁迫下的表达情况。【结果】序列分析表明,该基因编码框为729 bp,编码242个氨基酸,蛋白质分子量为27.405 ku,命名为SG-Ac CBF1,GenBank登录号为KM245571。氨基酸序列同源比对分析证实,SG-Ac CBF1属于CBF转录因子家族基因。系统发育树分析表明,SG-Ac CBF1与甜樱桃Pa DREB1的亲缘关系最近。亚细胞定位分析显示,SG-Ac CBF1蛋白定位于细胞核中。实时荧光定量PCR分析结果表明,低温、干旱、盐及ABA均能诱导SG-Ac CBF1基因表达。【结论】SG-Ac CBF1转录因子具有核定位功能,并参与了植物对逆境胁迫的调控。由于该转录因子属于家族基因,因此其对环境胁迫响应的强弱还有待探讨。

关键词(KeyWords)： 扁桃;Ac CBF1转录因子;亚细胞定位;表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31360473;31260186);; 新疆自治区十二五重大科技专项(201130102-1);; 新疆自治区果树学重点学科基金

作者(Authors): 张亮;李疆;帕提曼·阿布都热合曼;罗淑萍;田嘉;王敏;

DOI: 10.13925/j.cnki.gsxb.20150074

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