陈亚楠;毛运芝;冉慧;刘世尧;

• 1:西南大学园艺园林学院
• 2:南方山地园艺学教育部重点实验室

摘要(Abstract)：

【目的】了解我国皱皮木瓜不同地方品种资源果实有机酸组成与含量差异。【方法】通过甲基化衍生、GCMS检测进行我国10个主产区皱皮木瓜地方品种果实有机酸鉴定和含量测定。【结果】外标浓度与峰面积线性相关,各样品TIC基线平稳,成分峰分布均匀,分离度较高。全部样品共检出5大类43种有机酸,苹果酸含量最高。各样品总酸含量(w)在85.02～170.76 mg·g~(-1),山东临沂最高,陕西白河最低。10个样品共有成分33种,总酸含量与长链脂肪酸极显著正相关,与低碳羧酸显著正相关,与芳香酸负相关。聚类距离为5时10个地方品种资源共聚为4枝,四川仪陇、安徽宣城、陕西白河、广西南宁、湖北长阳聚为第1枝,重庆綦江、浙江淳安聚为第2枝,贵州正安、山东临沂样品聚为第3枝,云南景洪为第4枝。【结论】10个皱皮木瓜地方品种均属苹果酸型果实,果实有机酸组成种内差异较小,组分含量种间差异显著。

关键词(KeyWords)： 皱皮木瓜;地方品种资源;有机酸;甲酯化衍生;气相-质谱联用;差异分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31400272);; 中央高校基本业务费(XDJK2015C165)

作者(Author): 陈亚楠;毛运芝;冉慧;刘世尧;

Email:

DOI: 10.13925/j.cnki.gsxb.20190001

参考文献(References)：

• [1]中国科学院中国植物志编辑委员会.中国植物志(第36卷)[M].北京:科学出版社,1974:348.China Botanical Biography Compiling Committee. Flora of China(Volume 36)[M]. Beijing:Science Press:1974:348.
• [2]王嘉祥.山东皱皮木瓜品种分类探讨[J].园艺学报,2004,31(4):520-522.WANG Jiaxiang. Classification of flowering quince resource[J].Acta Horticulturae Sinica,2004,31(4):520-522.
• [3]王明明,王建华,宋振巧,李圣波,曲燕,刘静.木瓜属品种资源的数量分类研究[J].园艺学报,2009,36(5):701-710.WANG Mingming,WANG Jianhua,SONG Zhenqiao,LI Shengbo,QU Yan,LIU Jing. Studies on numerical classification of chaenomeles cultivars[J]. Acta Horticulturae Sinica,2009,36(5):701-710.
• [4]白志川,刘世尧,周志钦.药用木瓜规范化栽培及开发利用[M].北京:中国农业出版社,2012:59-61.BAI Zhichuan,LIU Shiyao,ZHOU Zhiqin. Standardized cultivation,development and utilization of medicinal Chaenomeles[M]. Beijing:Chinese Agricultural Publishing House,2012:59-61.
• [5] SHAO W H,LI Y J,DIAO S F,JIANG J M,DONG R X. Rapid classification of Chinese quince(Chaenomeles speciosa Nakai.)fruit provenance by near-infrared spectroscopy and multivariate calibration[J]. Analytical and Bioanalytical Chemistry,2017,409(1):115-120.
• [6]国家药典委员会编.中华人民共和国药典(2015年版一部)[M].北京:中国医药科技出版社,2015:61.National Pharmacopoeia Commission. People's Republic of China Pharmacopoeia(The first part of the 2015 Edition)[M]. Beijing:China Medical Science and Technology Press,2015:61.
• [7]国家药典委员会编.中华人民共和国药典(2010年版一部)[M].北京:中国医药科技出版社,2010:57.National Pharmacopoeia Commission. People's Republic of China Pharmacopoeia(The first part of the 2010 Edition)[M]. Beijing:China Medical Science and Technology Press,2010:57.
• [8] XIE X F,ZOU G L,LI C H. Antitumor and immunomodulatory activities of a water-soluble polysaccharide from Chaenomeles speciosa[J]. Carbohydrate Polymers,2015,132(5):323-329.
• [9] ZHANG L,CHENG Y X,LIU A L,WANG H D,WANG Y L,DU G H. Antioxidant,anti-Inflammatory and anti-influenza properties of components from Chaenomeles speciosa[J]. Molecules,2010,15(11):8507-8517.
• [10] MIAO J,ZHAO C C,LI X,CHEN X T,MAO X H,HUANG H H,WANG T T,GAO W Y. Chemical composition and bioactivities of two common Chaenomeles fruits in China:Chaenomeles speciosa and Chaenomeles sinensis[J]. Journal of Food Science,2016,81(8):2049-2058.
• [11] ROS J M,LAENCINA J,HELLIN P,JORDAN M J,VILA R,RUMPUNEN K. Characterization of juice in fruits of different Chaenomeles species[J]. LWT-Food Science and Technology,2004,37(3):301-307.
• [12] DU H,WU J,LI H,ZHONG P X,XU Y J,LI C H,JI K X,WANG L S. Polyphenols and triterpenes from Chaenomeles fruits:Chemical analysis and antioxidant activities assessment[J]. Food Chemistry,2013,141:4260-4268.
• [13]刘世尧,白志川,李加纳.皱皮木瓜与光皮木瓜药材品质多性状指标综合评价研究[J].中国中药杂志,2012,38(5):426-430.LIU Shiyao,BAI Zhichuan,LI Jiana. Comprehensive evaluation of multi-quality characteristic indexes of Chaenomeles speciosa and C. sinensis fruits[J].China Journal of Chinese Materia Medica,2012,38(5):426-430.
• [14]张上隆,陈昆松.果实品质形成与调控的分子生理[M].北京:中国农业出版社,2007:67-71.ZHANG Shanglong,CHEN Kunsong. Molecular physiology of fruit quality development and regulation[M]. Beijing:China Agriculture Press,2007:67-71.
• [15] SOCHOR J,SKUTKOVA H,BABULA P. Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars[J]. Molecules,2011,16(9):7428-7457.
• [16]刘世尧.不同产区皱皮木瓜有机酸组成及主要活性成分分离纯化研究[D].重庆:西南大学,2012.LIU Shiyao. Studying on organic acids compositon of Chaenomeles speciosa(Sweet)Nakai fruit from defferent producing area,isolation and purification of its'main functional components[D]. Chongqing:South West University,2012.
• [17]范建伟,黄博,宋根伟,何敬胜,梁鹏,王有为.不同产地木瓜总有机酸含量比较研究[C]//中华中医药学会.第九届中药鉴定学术会议,浙江建德,7.21-24,2008.北京:2008:486-488.FAN Jianwei,HUANG Bo,SONG Genwei,HE Jingsheng,LIANG Peng,WNAG Youwei. Comparative study on total organic acid content of Chaenomeles speciosa from different habitats[C]//China Association of Chinese Medicine. The Ninth Academic Conference on Chinese medicine identification of the Chinese traditioal medicine,July 21-24,2008.Jiande,Zhejiang. Beijing:2008:486-488.
• [18]卞京军,程密密,刘世尧,白志川,韦正鑫,蔡娟.皱皮木瓜皮渣齐墩果酸、熊果酸和总黄酮连续提取工艺研究[J].西南大学学报(自然科学版),2015,37(3):158-165.BIAN Jingjun,CHENG Mimi,LIU Shiyao,BAI Zhichuan,WEI Zhengxin,CAI Juan. Co-production extraction process for oleanolic acid,ursolic acid and total flavonoids from the peel pomece of Chaenomeles speciosa(Sweet)Nakai[J]. Journal of Southwest University(Natural Science Edition),2015,37(3):158-165.
• [19]蔡娟,刘世尧,韦正鑫,张丹,潘雪珍,卞京军,白志川.皱皮木瓜皮渣齐墩果酸和熊果酸提取工艺优化研究[J],食品工业科技,2015,38(2):282-290.CAI Juan,LIU Shiyao,WEI Zhengxin,ZHANG Dan,PAN Xuezhen,BIAN Jingjun,BAI Zhichuan. Study on the conditions of extracting oleanolic acid and ursolic acid from residue of Chaenomeles speciosa(Sweet)Nakai[J]. Science and Technology of Food Industry,2015,38(2):282-290.
• [20]高诚伟,康勇,雷泽模,段志红,李蕾.皱皮木瓜中有机酸的研究[J].云南大学学报(自然科学版),1999,21(4):319-321.GAO Chengwei,KANG Yong,LEI Zemo,DUAN Zhihong,LI Lei. The studies on the acidic constituents in the fresh-fruit of Chaenomeles spciosa[J]. Journal of Yunnan University(Natural Science Edition),1999,21(4):319-321.
• [21]龚复俊,陈玲,卢笑丛,王有为.皱皮木瓜果实中有机酸成分的GC-MS分析[J].植物资源与环境学报,2005,14(4):55-56.GONG Fujun,CHEN Ling,LU Xiaocong,WANG Youwei. GCMS analyzing of organic acids in Chaenomeles speciosa fruits[J]. Journal of Plant Resources and Environment,2005,14(4):55-56.
• [22] ZHANG S Y,HAN L Y,ZHANG H,XIN H L. Chaenomeles speciosa:a review of chemistry and pharmacology[J]. Biomedical Reports,2014,2:12-18.
• [23]迟广俊,刘俊辉,鲍峰玉,张文娟,秦炎龙,孙世豪,宗永立,张建勋,邱宝平,刘晓旭,郭鹏,赵秀杰,柴国璧,张启东.初烤烟叶提取物中酸味关键成分的感官导向分析[J].烟草科技,2015,48(12):27-32.CHI Guangjun,LIU Junhui,BAO Fengyu,ZHANG Wenjuan,QIN Yanlong,SUN Shihao,ZONG Yongli,ZHANG Jianxun,QIU Baoping,LIU Xiaoxu,GUO Peng,ZHAO Xiujie,CHAI Guobi,ZHANG Qidong. Sensory-oriented analysis of key sour taste components in cured tobacco extract[J]. Tobacco Science&Technology,2015,48(12):27-32.
• [24]鄢凌,傅宏鑫,王旭东,孙亚琴,戴建英,修志龙.生物基有机酸提取分离技术研究进展[J].过程工程学报,2018,18(1):1-10.YAN Ling,FU Hongxin,WANG Xudong,SUN Yaqin,DAI Jianying,XIU Zhilong. Recent advances on recovery and separation of biomass-based organic acids[J]. The Chinese Journal of Process Engineering,2018,18(1):1-10.
• [25]刘晓棠,张卫明,姜洪芳,单承莺.不同种类木瓜样品的总有机酸含量测定[J].食品研究与开发,2010,31(1):100-102.LIU Xiaotang,ZHANG Weiming,JIANG Hongfang,SHAN Chengying. Determination of tolal organic acids in different samples of fructus Chaenomaelis[J]. Food Research and Development,2010,31(1):100-102.
• [26]靳志飞,陈红.响应面法优化超声辅助提取果梅果实有机酸工艺[J].湖北农业科学,2016,55(7):1782-1787.JIN Zhifei,CHEN Hong. Process optimization for ultrasonicassisted solvent extraction of organic acid from Prunus mume Sieb. et. Zucc. fruit by response surface method[J]. Hubei Agriculture Science,2016,55(7):1782-1787.
• [27]田林锋,胡继伟,黄先飞,李存雄,林陶.反相高效液相色谱法快速测定梨中7种有机酸含量[J].食品研究与开发,2010,31(2):126-128.TIAN Linfeng,HU Jiwei,HUANG Xianfei,LI Cunxiong,LIN Tao. High efficiencey determination of organic acids in pears with reversied-phase high performance liquid chromatography[J]. Food Research and Development,2010,31(2):126-128.
• [28]庞荣丽,方金豹,郭琳琳,谢汉忠.水果果实中主要有机酸提取条件的优化[J].中国农业科学,2014,47(13):2625-2633.PANG Rongli,FANG Jinbao,GUO Linlin,XIE Hanzhong. Extraction conditions optimization of main organic acids from fruits[J]. Scientia Agricultura Sinica,2014,47(13):2625-2633.
• [29]李珊,阿依姑丽·艾合麦提,孙鹏英,提扎尔·艾孜木江,王妙颖. RP-HPLC法同时测定新疆野山杏果肉中10种有机酸成分[J].食品工业科技,2017,38(20):250-255.LI Shan,AYGUL·AHMT,SUN Pengying,ENTIZAR·AZMJ,WANG Miaoying. Simultaneous determination of ten acids in Xinjiang wild apricot with reverse-phase high performance liquid chromatography[J]. Science and Technology of Food Industry,2017,38(20):250-255.
• [30]王韦岗,唐双双,陆源.气相色谱法测定食品中12种有机酸[J].理化检验(化学分册),2017,53(11):1313-1317.WANG Weigang,TANG Shuangshuang,LU Yuan. GC determination of 12 organic acids in foods[J]. Physical Testing and Chemical Analysis(Part B:Chemical Analysis),2017,53(11):1313-1317.
• [31]张毅,刘伟,李桂祥,董晓民,徐兴东,李厚勇,李圣波,刘宗钊.山东木瓜属品种资源调查[J].中国园艺文摘,2015,31(10):1-11.ZHANG Yi,LIU Wei,LI Guixiang DONG Xiaomin,XU Xingdong,LI Houyong,LI Shengbo,LIU Zongzhao. Germplasm resources of Chaenomeles L. in Shandong[J].Chinese Horticulture Abstract,2015,31(10):1-11.