王龙;薛华柏;杨健;王苏珂;苏艳丽;李秀根;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】通过鉴定80份梨种质资源SSR指纹数据,构建梨品种指纹图谱数库表,为实现梨种质资源鉴定及新品种DUS测试的可视化和简便化奠定数据基础。【方法】利用荧光标记技术检测SSR位点,并对SSR位点基因数目进行统计,SSR位点排序,并对供试品种进行排序区分和数据标示,构建了80个梨品种的特征指纹数据表。【结果】12对引物中,可利用NH039A、NH007b、NAUpy97a、NB105a、NB141b、NAUpy82r 6对引物区分所有供试品种,可作为区分这80个梨品种的核心引物。【结论】筛选出了区分供试80个梨品种的核心引物,为梨指纹图谱数据库的共建和共享提供了数据基础。

关键词(KeyWords)： 梨;分子标记;SSR;品种鉴定;指纹数据表

Abstract:

Keywords:

基金项目(Foundation): 现代农业(梨)产业技术体系建设专项(CARS-29);; 河南省自然科学基金(162300410150)

作者(Author): 王龙;薛华柏;杨健;王苏珂;苏艳丽;李秀根;

Email:

参考文献(References)：

• [1]孙云蔚.中国果树史与果树资源[M].上海:上海科学技术出版社,1983.SUN Yunwei.The fruit history and resources in China[M].Shanghai:Shanghai Science and Technology Press,1983.
• [2]田路明,曹玉芬,董星光.中国梨种质创新研究进展:梨产业技术研究与应用[M].北京:中国农业出版社,2010:29-34.TIAN Luming,CAO Yufen,DONG Xingguang.The development of Chinese pear breeding:the research and utility of pear industry technology[M].Beijing;Chinese Agriculture Press,2010:29-34.
• [3]李秀根,杨健,王龙,王苏珂.我国近30a梨育种研究进展与今后工作建议[J].果树学报,2010,27(6):987-994.LI Xiugen,YANG Jian,WANG Long,WANG Suke.Progress and proposal in research of pear breeding in China[J].Journal of Fruit Science,2010,27(6):987-994.
• [4]高源,田路明,刘凤之,曹玉芬.利用SSR荧光标记构建92个梨品种指纹图谱[J].园艺学报,2012,39(8):1437-1446.GAO yuan,TIAN Luming,LIU Fengzhi,CAO Yufen.Using the SSR fluorescent labeling to establish SSR fingerprints for 92 cultivars in Pyrus[J].Acta Horticulturae Sinica,2012,39(8):1437-1446.
• [5]麻丽颖,孔德仓,刘华波,王斯琪,李颖岳,庞晓明.36份枣品种SSR指纹图谱的构建[J].园艺学报,2012,39(4):647-654.MA Liying,KONG Decang,LIU Huabo,WANG Siqi,LI Yingyue,PANG Xiaoming.Construction of SSR fingerprint on 36 Chinese jujube cultivars[J].Acta Horticulturae Sinica,2012,39(4):647-654.
• [6]姚全胜,詹儒林,黄丽芳,王松标,武红霞,马小卫,邢姗姗,雷新涛.11个芒果品种SSR指纹图谱的构建与品种鉴别[J].热带作物学报,2009,30(11):1572-1576.YAO Quansheng,ZHAN Rulin,HUANG Lifang,WANG Songbiao,WU Hongxia,MA Xiaowei,XING Shanshan,LEI Xintao.Construction of fingerprinting map and identification of cultivars for 11 mango cultivars by SSR markers[J].Chinese Journal of Tropical Crops,2009,30(11):1572-1576
• [7]王壮伟,赵密珍,袁骥,吴伟民,钱亚明.38个欧美草莓栽培品种SSR指纹图谱的构建[J].果树学报,2011,28(6):1032-1037.WANG Zhuangwei,ZHAO Mizhen,YUAN Ji,WU Weimin,QIAN Yaming.Establishment of SSR fingerprinting database for 38 cultivars of strawberry(Fragaria ananassa)native to Europe and America[J].Journal of Fruit Science,2011,28(6):1032-1037.
• [8]岳娜,胡倩倩,方星,邱学林,储春荣,祁红丽,孟祥勋.枇杷种质资源SSR标记指纹图谱的构建[J].中国农学通报,2012,28(6):186-189.YUE Na,HU Qianqian,FANG Xing,QIU Xuelin,CHU Chunrong,QI Hongli,MENG Xiangxun.Fingerprinting patterns of SSR markers constructed for 33 loquat[Eriobotrya japonica(Thunb.)Lindl.]germplasms[J].Chinese Agricultural Science Bulletin,2012,28(6):186-189.
• [9]艾呈祥,张力思,魏海蓉,苑克俊,金松南,刘庆忠.甜樱桃品种SSR指纹图谱数据库的建立[J].中国农学通报,2007,23(5):55-58.AI Chengxiang,ZHANG Lisi,WEI Hairong,YUAN Kejun,JIN Songnan,LIU Qingzhong.Construction of molecular fingerprinting database for sweet cherry using SSR markers[J].Chinese Agricultural Science Bulletin,2007,23(5):55-58.
• [10]BAO L,CHEN K S,ZHANG D,CAO,Y F,YAMAMOTO T,TENG Y W.Genetic diversity and similarity of pear(Pyrus L.)cultivars native to East Asia revealed by SSR(simple sequence repeat)markers[J].Genetic Resources and Crop Evolution,2007,54:959-971.
• [11]路娟,吴俊,张绍玲,吴华清,张妤艳.不同系统梨种质遗传多样性与分类关系的SSR分析[J].南京农业大学学报,2011,34(2):38-46.LU Juan,WU Jun,ZHANG Shaoling,WU Huaqing,ZHANG Yuyan.Genetic diversity and polygentic relationship among pears reveled by SSR analysis[J].Journal of Nanjing Agricultural University,2011,34(2):38-46.
• [12]JIANG Z W,TANG F Y.Assessment of genetic diversity of Chinese sand pear landraces(Pyrus pyrifolia Nakai)[J].Hortscience,2009,44(3),619-626.
• [13]WüNSCH A,HORMAZA J I.Characterization of variability and genetic similarity of European pear using microsatellite loci developed in apple[J].Scientia Horticulturae,2007,113:37-43.
• [14]ZHANG Q,LI J,ZHAO Y B,KORBAN S S,HAN Y P.Evaluation of genetic diversity in Chinese Wild apple species along with apple cultivars using SSR markers[J].Plant Molecular Biology Reporter,2011,30:539-546.
• [15]YAO L H,ZHENG X Y,CAI D Y,GAO Y,WANG K,CAO Y F,TENG Y W.Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus[J].Genetic Resources and Crop Evolution,2010,57:841-851.
• [16]SEHIC J,GARKAVA-GUSTAVSSON L,FERNáNDEZFERNáNDEZ F,NYBOM H.Genetic diversity in a collection of European pear(Pyrus communis)cultivars determined with SSR markers chosen by ECPGR[J].Scientia Horticulturae,2012,145:39-45.
• [17]SONG Y,FAN L,CHEN H,ZHANG M Y,MA QI Q,ZHANG S L,WU J.Identifying genetic diversity and a preliminary core collection of Pyrus pyrifolia cultivars by a genome-wide set of SSR markers[J].Scientia Horticulturae,2014,167:5-16.
• [18]曹玉芬,刘凤之,高源,姜立杰,王昆,马智勇,张开春.梨栽培品种SSR鉴定及遗传多样性[J].园艺学报,2007,34(2):305-310.CAO Yufen,LIU Fengzhi,GAO Yuan,JIANG Lijie,WANG Kun,MA Zhiyong,ZHANG Kaichun.SSR analysis of genetic diversity of pear cultivars[J].Acta Horticulturae Sinica,2007,34(2):305-310.
• [19]薛华柏,杨健,王龙,王苏珂,张惠蓉,乔玉山,章镇,李秀根.29个梨品种SSR特征指纹数据表的构建[J].果树学报,2015,32(6):1028-1035.XUE Huabo,YANG Jian,WANG Long,WANG Suke,ZHANG Huirong,QIAO Yushan,ZHANG Zhen,LI Xiugen.Construction of SSR characteristic fingerprinting data table for 29 pear cultivars[J].Journal of Fruit Science,2015,32(6):1028-1035.
• [20]陈昌文,曹珂,王力荣,朱更瑞,方伟超.中国桃主要品种资源及其野生近缘种的分子身份证构建[J].中国农业科学,2011,44(10):2081-2093.CHEN Changwen,CAO Ke,WANG Lirong,ZHU Gengrui,Fang Weichao.Molecular ID establishment of main China peach varieties and peachrelated species[J].Scientia Agricultura Sinica,2011,44(10):2081-2093.
• [21]高源,刘凤之,王昆,王大江,龚欣,刘立军.苹果部分种质资源分子身份证的构建[J].中国农业科学,2015,48(19):3887-3898.GAO Yuan,LIU Fengzhi,WANG Kun,WANG Dajiang,GONG Xin,LIU Lijun.Establishment of molecular ID for some apple germplasm resources[J].Scientia Agricultura Sinica,2015,48(19):3887-3898.
• [22]张靖国,田瑞,陈启亮,杨晓平,胡红菊.基于SSR标记的梨栽培品种分子身份证的构建[J].华中农业大学学报,2014,33(1):12-17.ZHANG Jingguo,TIAN Rui,CHEN Qiliang,YANG Xiaoping,HU Hongju.Establishment of molecular ID for pear cultivars based on SSR markers[J].Journal of Huazhong Agricultural University,2014,33(1):12-17.
• [23]杜晶晶,刘国银,魏亚军,刘德兵,杨小振.基于SSR标记构建葡萄种质资源分子身份证.植物研究,2013,33(2):232-237.DU Jingjing,LIU Guoyin,WEI Yajun,LIU Debing,YANG Xiaozhen.Establishment of molecular ID for grape germplasm based on SSR markers[J].Bulletin of Botanical Research,2013,33(2):232-237.
• [24]YAMAMOTO T,KIMURA T,SHODA M,BAN Y,HAYAHSI T,MATSUTA N.Development of microsatellite markers in the Japanese pear(Pyrus pyrifolia Nakai)[J].Molecular Ecology Notes2002,2:14-16.
• [25]CHEN H,SONG Y,LI L T,KHAN A M,LI X G,KORBAN S S,WU J,ZHANG S L.Construction of a high-density simple sequence repeat consensus genetic map for pear(Pyrus spp.)[J].Plant Molecular Biology Reporter,2014,33:316-325.
• [26]YAMAMOTO T,KIMURA T,SHODA M,IMAI T,SAITO T,SAWAMURA Y,KOTOBUKI K,HAYASHI T,MATSUTA N.Genetic linkage maps constructed by using an interspecific cross between Japanese and European pears[J].Theoretical and Applied Genetics,2002,106:9-18.
• [27]LIEBHARD R,GIANFRANCESCHI L,KOLLER B,RYDER C D,TARCHINI R,WEG E V D,GESSLER C.Development and characterisation of 140 new microsatellites in apple(Malus×domestica Borkh.)[J].Molecular Breeding,2002,10:217-241.