董易之,陈炳旭,徐淑,李鹏燕,张晓薇

• 1:广东省农业科学院植物保护研究所
• 2:华中农业大学植物科学技术学院

摘要(Abstract)：

【目的】为了探明抗感蛀果害虫板栗品种挥发物化学成分及其含量的差异,【方法】从抗感虫板栗品种果实和叶片中提取挥发物,并应用气相色谱-质谱联用技术对其化学成分进行分析。【结果】分别从抗虫品种河源油栗和感虫品种‘农大1号’果、叶挥发物中分别检测到111、142、138和134种化学成分。其中,相对含量超过0.5%的成分分别有32、30、20和20种,分别占挥发物总量的83.03%、84.96%、81.73%和83.95%,主要由绿叶气味、芳香族、单萜、倍半萜和杂环类化合物组成。‘河源油栗’果实挥发物主要成分为(E)-2-己烯醛(10.59%)、正己醇(10.42%)、糠醇(8.07%)、苯乙醛(5.63%)和安息香醇(4.76%),叶片中为2-乙氧丙烷(19.73%)、(Z)-3-己烯-1-醇(14.4%)、正己醇(10.23%)、(E)-2-己烯-1-醇(6.83%)和β-桉叶醇(4.76%);‘农大1号’果实挥发物为2-乙氧丙烷(49.71%)、糠醛(4.11%)、(Z)-3-己烯-1-醇(3.89%)、β-桉叶醇(3.19%)和苯乙醛(2.44%),叶片中为(E)-2-己烯醛(20.18%)、正己醇(15.28%)、(E)-2-己烯-1-醇(12.01%)、2-乙氧丙烷(6.82%)和苯甲醇(6.16%)。其中,‘河源油栗’果实挥发物中的绿叶气味和杂环类化合物含量较‘农大1号’果实高,分别占挥发物总量的32.55%和13.79%,而两类成分在后者中仅占9.96%和5.23%。‘河源油栗’果实挥发物的3个特有成分(E)-2-己烯醛、1,2,3,4,4a,5,6,7-八氢-4a-甲基-2-奈酚和3-戊醇含量很高,分别占挥发物总量的10.59%、4.66%和3.53%。但‘河源油栗’果实挥发物不含2-乙氧丙烷、(Z)-3-己烯-1-醇和甲苯,而这3个成分在‘农大1号’果实中分别占49.72%、3.89%和2.32%。【结论】抗感虫板栗品种果、叶挥发物化学成分及其含量存在较大差异。

关键词(KeyWords)： 板栗;抗虫;感虫;挥发物;GC-MS分析

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金项目(10451064001006259);; 广东省农业攻关项目(2011B020308003)

作者(Author): 董易之,陈炳旭,徐淑,李鹏燕,张晓薇

DOI: 10.13925/j.cnki.gsxb.2012.06.022

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