曹慧,程姣姣,刘春香,李媛媛,邹岩梅,束怀瑞

• 1:山东省高校生物化学与分子生物学重点实验室·潍坊学院
• 2:国家苹果工程技术研究中心

摘要(Abstract)：

【目的】为了探明类caspase蛋白酶参与植物PCD的机制,【方法】以八棱海棠(Malus robusta Rehd.)实生苗为试验材料,对植株进行轻度干旱胁迫,检测叶片PCD的发生,并应用RT-PCR技术克隆类caspase基因。【结果】结果表明,轻度干旱胁迫后3周的叶片出现细胞染色质凝聚、胞质皱缩、细胞核变形等细胞凋亡的形态学特征。提取叶片DNA,观察到DNA呈现明显的"DNA Ladder",表现出典型的细胞程序性死亡的生化特征。在此基础上提取叶片RNA,采用RT-PCR获得长度为555 bp的类caspase基因片段。【结论】经同源性分析该片段与其他果树的同源序列相似性超过80%,提示该区段为保守序列。比对苹果基因组,八棱海棠基因组中存在半胱氨酸蛋白酶基因,该基因为单拷贝,并且存在2个同源基因,这为揭示干旱胁迫下八棱海棠调控PCD的机理及环境适应的分子机制奠定了基础。

关键词(KeyWords)： 八棱海棠;干旱胁迫;细胞程序性死亡;类caspase

Abstract:

Keywords:

基金项目(Foundation): 国家现代苹果产业技术体系;; 山东省自然科学基金(Y2008D60);; 山东省中青年科学家奖励基金(2005BS02012)

作者(Author): 曹慧,程姣姣,刘春香,李媛媛,邹岩梅,束怀瑞

DOI: 10.13925/j.cnki.gsxb.2012.04.009

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