方智振;姜翠翠;周丹蓉;潘少霖;林炎娟;叶新福;

• 1:福建省农业科学院果树研究所·福建省落叶果树工程技术研究中心

摘要(Abstract)：

【目的】克隆在采后‘秋姬李’果皮积累花色苷过程中高表达的MYB抑制因子PsMYB18基因,研究其序列特征、表达特点与功能。【方法】以‘秋姬李’为试材,采用qRT-PCR分析不同温度和光照处理条件下‘秋姬李’果皮中PsMYB18基因的转录水平,采用RT-PCR克隆PsMYB18基因,并通过烟草叶片瞬时表达试验分析PsMYB18的功能。【结果】q RT-PCR分析表明20℃和光照处理可促进‘秋姬李’果皮PsMYB18基因表达。PsMYB18基因的开放阅读框(ORF)为702 bp,编码233个氨基酸的蛋白。进化树分析表明PsMYB18与其他植物的花色苷合成抑制因子亲缘关系较近。序列比对结果表明其具有保守的R2R3结构域和抑制基序C1和C2。烟草瞬时表达试验表明,PsMYB18可抑制正调控因子PsMYB10.1和PsbHLH3的花色苷合成诱导功能。【结论】‘秋姬李’PsMYB18为花色苷合成抑制因子。

关键词(KeyWords)： ‘秋姬李’;PsMYB18;花色苷合成抑制因子

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31801916);; 福建省省属公益类科研院所基本科研专项(2018R1013-1);; 福建农业科学院青年英才专项(YC2015-9);; 福建省农业科学院创新团队(STIT2017-1-4)

作者(Author): 方智振;姜翠翠;周丹蓉;潘少霖;林炎娟;叶新福;

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