孙洪圳;吕海涛;柏素花;李保华;祝军;戴洪义;

• 1:青岛农业大学园林园艺学院
• 2:青岛农业大学化学与药学院
• 3:青岛农业大学生命科学学院
• 4:青岛农业大学农学与植物保护学院

摘要(Abstract)：

【目的】为了建立高效液相色谱法(HPLC)同时测定苹果树皮中没食子酸、儿茶酚、儿茶素、绿原酸、咖啡酸、表儿茶素、香豆酸、阿魏酸、芦丁、根皮苷、槲皮素、根皮素12种酚类化合物的方法,【方法】选用Eclipse XDB C18色谱柱(150 mm×4.6 mm i.d,5μm),以甲醇和1%乙酸溶液作为流动相进行梯度洗脱,利用VWD检测器,在各种酚类物质的最优吸收波长下进行检测,【结果】得到12种酚类化合物的线性回归方程,相关系数均在0.999以上,检出限(3RSN)分别为0.02、0.02、0.03、0.01、0.02、0.03、0.01、0.01、0.03、0.03、0.05和0.01 mg.L-1。色谱测定方法和包含样品处理方法的加标回收率分别在56.54%～98.83%和90.95%～100.90%。【结论】整个提取和分析测定流程简便、快速、准确,适合苹果树皮中酚类化合物的分析测定。

关键词(KeyWords)： 苹果树皮;高效液相色谱;酚类化合物

Abstract:

Keywords:

基金项目(Foundation): 国家现代苹果产业技术体系项目(CARS-28-01-07)

作者(Authors): 孙洪圳;吕海涛;柏素花;李保华;祝军;戴洪义;

DOI: 10.13925/j.cnki.gsxb.2012.05.036

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