甜樱桃SSR体系的建立、优化及应用Establishment,optimization and application of SSR system in sweet cherry
张琪静;张新忠;李贺;谷大军;李春敏;张志宏;
摘要(Abstract)：
为摸索适宜甜樱桃的SSR反应体系,利用正交设计对Mg2+、dNTPs、引物、Taq酶和模板DNA等5种因素4个水平进行筛选和优化,20μL反应体系中,Mg2+、dNTPs和引物的最适浓度为1.5mmol/L、0.2mmol/L、0.4μmol/L,Taq酶宜加入1U,模板DNA应加入10～40ng;引物的最佳退火温度为56.0～62.8℃。用10对引物及建成的反应体系对10个供试品种扩增,6%的非变性聚丙烯酰胺凝胶电泳检测,品种间DNA谱带多态性丰富,共有29个等位位点,证实该体系稳定可靠。
关键词(KeyWords)： 甜樱桃;正交试验;SSR;反应体系
基金项目(Foundation):
作者(Author): 张琪静;张新忠;李贺;谷大军;李春敏;张志宏;
Email:
DOI:
参考文献(References)：
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