中国樱桃S-RNase基因PCR扩增技术体系的建立Establishment of PCR reaction system of S-RNase gene in Chinese cherry cultivars(Cerasus pseudocerasus)
李晓;吴俊;张绍铃;
摘要(Abstract)：
通过对TaqDNA聚合酶、Mg2+、dNTP、通用引物、模板DNA浓度等反应参数的系统研究,建立了中国樱桃S-RNase基因特异PCR扩增体系。该体系反应的总体积25μL,其中Taq酶1.25U,MgCl22.5mmol/L,dNTP0.15mmol/L,通用引物0.25μmol/L,模板DNA 50 ng。反应程序为:94℃预变性3 min;33个循环的94℃变性1 min,56℃退火45 s,72℃延伸1.5 min;最后72℃延伸10 min。利用优化的扩增体系在中国樱桃的不同品种中获得有效扩增,进一步证明了该体系具有良好的稳定性和重复性。
关键词(KeyWords)： 中国樱桃;S-RNase基因;PCR扩增体系
基金项目(Foundation):
作者(Author): 李晓;吴俊;张绍铃;
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参考文献(References)：
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