用于多基因表达检测的猕猴桃macroarray阵列Preparation of kiwifruit macroarray for high throughput gene expression analysis
张波;徐昌杰;陈昆松;
摘要(Abstract)：
基因阵列是开展高通量功能基因组学研究的有效手段之一。对适用于多年生果树猕猴桃的macroarray阵列进行了探索,构建了含有42个基因的macroarray阵列,与α-32P标记的cDNA探针杂交产生清晰信号,有效分辨了不同基因或基因家族不同成员的表达差异。DNase处理可去除基因组DNA污染,提高结果准确性。
关键词(KeyWords)： 猕猴桃;macroarray;基因表达;构建
基金项目(Foundation): 国家自然科学基金(30571284);; 高等学校博士学科点专项科研基金(20040335022);; 高等学校学科创新引智计划(B06014)
作者(Author): 张波;徐昌杰;陈昆松;
Email:
参考文献(References)：
- [1]SASAKI Y,ASAMIZU E SHIBATA D.Monitoring of methyl jas-monate-responsive genes in Arabidopsis by cDNA macroarray:self-activation of jasmonic acid biosynthesis and crosstalk with other phytohormone signalingpathways[J].DNAResearch,2001,8:153-161.
- [2]NOGUEIRA F T S,DE ROSA V E,MENOSSI J M,ULIAN E C,ARRUDA P.RNA expression profiles and data mining of sugarcane response to lowtemperature[J].Plant Physiology,2003,132:1811-1824.
- [3]BELLIN D,SCHULZ B,SOERENSEN T R,SALAMINI F,SCHNEI-DER K.Transcript profiles at different growth stages and tap-root zones identify correlated developmental and metabolic pathways of sugar beet[J].Journal ofExperimental Botany,2007,58:699-715.
- [4]TRAINOTTI L,ZANIN D,CASADORA G.A cell wall-oriented ge-nomic approach reveals a new and un expected complexity of the softening in peaches[J].Journal of Experimental Botany,2003,54:1821-1832.
- [5]MOZORUK J,HUNNICUTT L E,GAVE R D,HUNTER W B,BAUSHER MG.Profiling transcriptional changes in Citrus sinensis(L.)Osbeck challenged by herbivory from the xylem-feeding leafhopper Homalodisca coagulate(Say)by cDNA macroarray analy-sis[J].Plant Science,2006,170:1068-1080.
- [6]ZHANG B,CHEN K S,BOWEN J,ALLAN A,ESPLEY R,KARUNAIRETNAMS,FERGUSONI.Differential expression with-in the LOX gene family in ripening kiwifruit[J].Journal of Experi-mental Botany,2006,57:3825-3836.
- [7]NGUYENC,ROCHAD,GRANJEAUDS,BALDITM,BERNARD K,NAQUET P,JORDAN B R.Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cD-NAclones[J].Genomics,1995,29:207-216.
- [8]THE ARABIDOPSIS GENOME INITIATIVE.Analysis of the genome sequenceofthefloweringplantArabidopsisthaliana[J].Nature,2000,408:796-815.
- [9]DE ARAUJO P G,ROSSI MR,JESUS E M,SACCARO J R N L,KAJIHARA D,MASSA R,DEFLIX J MDRUMMOND R D,FAL-COM C,CHABREGAS S M,ULIAN E C,MENOSSI M,VAN SLUYSMA.Transcriptionallyactive transposable elements in recent hybrid sugarcane[J].The Plant Journal,2005,44:707-717.
- [10]BALCZUNC,BUNSE A,NOWROUSIANM,KORBELA,GLANZ S,K"CK U.DNA macroarray and real-time PCR analysis of two nuclear photosystem I mutants from Chlamydomonas reinhardtii re-veal downregulation of Lhcb genes but different regulation of Lhca genes[J].Biochimica et Biophysica Acta,2005,1732:62-68.
- [11]VILANINE F,PALAUQUI J C,AMSELEMJ,KUSIAK C,LEMOINE R,DINANT S.Towards deciphering phloem:a transcriptome analy-sis of the phloem of Apium graveolens[J].The Plant Journal,2003,36:67-81.
- [12]MILLER N A,GONG Q,BRYAN R,RUVOLO M,TURNER L A,LABRIE S T.Cross-hybridization of closely related genes on high-densitymacroarrays[J].BioTechniques,2002,32:620-625.