用于多基因表达检测的猕猴桃macroarray阵列Preparation of kiwifruit macroarray for high throughput gene expression analysis
张波;徐昌杰;陈昆松;
摘要(Abstract)：
基因阵列是开展高通量功能基因组学研究的有效手段之一。对适用于多年生果树猕猴桃的macroarray阵列进行了探索,构建了含有42个基因的macroarray阵列,与α-32P标记的cDNA探针杂交产生清晰信号,有效分辨了不同基因或基因家族不同成员的表达差异。DNase处理可去除基因组DNA污染,提高结果准确性。
关键词(KeyWords)： 猕猴桃;macroarray;基因表达;构建
基金项目(Foundation): 国家自然科学基金(30571284);; 高等学校博士学科点专项科研基金(20040335022);; 高等学校学科创新引智计划(B06014)
作者(Author): 张波;徐昌杰;陈昆松;
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参考文献(References)：
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