赵烨;国静;王甲威;徐丽;谭钺;陈新;魏海蓉;朱东姿;刘庆忠;宗晓娟;

• 1:山东省果树研究所·山东省果树生物技术育种重点实验室
• 2:山东农业大学林学院

摘要(Abstract)：

【目的】探明PcMPK3基因的表达调控规律。【方法】利用染色体步移技术从甜樱桃矮化砧木Gisela 6中克隆PcMPK3基因的启动子序列PcMPK3pro。利用Neural Network Promotor Prediction、softberry、PLACE和PlantCARE网站在线预测PcMPK3基因的基础启动子、转录起始位点和顺式作用元件。将PcMPK3pro定向替换植物表达载体pBI121-SN1的CaMV35S组成型启动子,构建重组表达载体pBI-PcMPK3pro:GUS,瞬时转化烟草叶片。【结果】结果表明,PcMPK3pro含有启动子核心元件TATA-box和CAAT-box等多种响应胁迫的顺式作用元件。受病原菌丁香假单胞菌番茄致病变种(Pseudomonas syringae pv. tomato DC3000,Pst DC3000)侵染,PcMPK3pro能驱动GUS报告基因表达且GUS酶活性显著提高。【结论】推测PcMPK3基因参与植物响应病原菌感染的胁迫过程。

关键词(KeyWords)： 甜樱桃;砧木;PcMPK3;启动子;GUS基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(31601732);国家自然科学基金面上项目(31770706);; “十二五”国家科技支撑计划(2013BAD02B03-3-2);; 山东省现代农业产业技术体系果品创新团队(SDAIT-06-04)

作者(Author): 赵烨;国静;王甲威;徐丽;谭钺;陈新;魏海蓉;朱东姿;刘庆忠;宗晓娟;

Email:

DOI: 10.13925/j.cnki.gsxb.20180411

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