荔枝龙眼细胞悬浮培养和转基因研究(英文)A study of cell suspension cultuer and genetic transformation of litchi and longan
郑启发,胡桂兵,陈大成,黄自然
摘要(Abstract)：
应用正交设计研究了IBA、6-BA、GA3和CM等植物生长调节剂在龙眼(Dimocarpus longana Lour.)与荔枝 (Litchi chinensis Sonn.)细胞悬浮培养中的作用,并就悬浮培养细胞对Km的敏感性以及用金刚沙对胚性愈伤组织在 液体状态下进行创伤和用分别带有AP1和APBD基因的农杆菌对创伤的愈伤组织进行遗传转化进行了探讨。结果 表明:IBA、6-BA、GA3和CM均可显著地提高悬浮培养系的产量,但GA3导致细胞的快速生长和褐变。悬浮培养系 细胞在液体培养基中比在固体培养基中对Km较敏感。用金刚沙对胚性愈伤组织在液体状态下进行创伤后进行农 杆菌介导转化可以得到更多的Km抗性胚状体。PCR检测结果显示,AP1及AP-D基因均已转入龙眼的胚状体中。
关键词(KeyWords)： 荔枝;龙眼;正交设计;悬浮培养;遗传转化
基金项目(Foundation): Key Research Prograns'High Technology and Basic Research'(1996-2000)of the Ministry of Agruculture,Peaple'Republic of China(95-15-03-02) The'CHEN Li-wen'Foundation of South China Agricultural University (5300-K99047).
作者(Author): 郑启发,胡桂兵,陈大成,黄自然
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