白芝兰;李果果;吴晓冉;李国怀;

• 1:园艺植物生物学教育部重点实验室·华中农业大学

摘要(Abstract)：

【目的】为了初步建立野生‘毛桃1号’叶片愈伤组织诱导、保存及不定芽再生技术体系,【方法】以野生‘毛桃1号’试管苗幼嫩叶片为外植体,探讨了基本培养基、激素配比等一系列因素对‘毛桃1号’叶片愈伤组织诱导和保存的影响及TDZ浓度对愈伤组织不定芽再生的影响。【结果】结果表明,适合‘毛桃1号’叶片愈伤组织诱导的培养基为LP+6-BA 1.0 mg.L-1+2,4-D 3.0 mg.L-1+AgNO30.5 mg.L-1+蔗糖30.0 g.L-1+琼脂6.0 g.L-1;保存的培养基为LP+2,4-D1.5 mg.L-1+CH 0.4 g.L-1+蔗糖30 g.L-1+CA 3.0 g.L-1或PVP 3.0 g.L-1+琼脂5.8 g.L-1,且采用持续暗培养保存,每28 d继代1次。愈伤组织在SH+TDZ 3.0 mg.L-1+NAA 0.3 mg.L-1+蔗糖30 g.L-1+琼脂6.0 g.L-1的培养基上可再生不定芽,再生率为6.83%。【结论】初步建立了‘毛桃1号’叶片愈伤组织诱导、保存及不定芽再生体系,为桃愈伤组织再生和遗传转化的研究奠定了基础。

关键词(KeyWords)： ‘毛桃1号’;愈伤组织;植株再生

Abstract:

Keywords:

基金项目(Foundation): 国家桃产业技术体系建设专项(CARS-31-2-4)

作者(Authors): 白芝兰;李果果;吴晓冉;李国怀;

DOI: 10.13925/j.cnki.gsxb.2012.05.021

参考文献(References)：

• [1]LIU Chang-hong,JIA Ke-gong,ZHU Li-xin,LI Hui,YE Hang,WANG Yan-chun.Resistance of Prunus persica to crown gall dis-ease[J].Journal of China Agricultural University,2009,14(5):68-71.刘常红,贾克功,朱立新,李晖,叶航,王衍纯.毛桃对根癌病的抗性研究[J].中国农业大学学报,2009,14(5):68-71.
• [2]YE Hang,JIAN Heng,ZHU Li-xin,WANG Wen-jun,JIA Ke-gong.The research on resistency of four peach rootstock to Meloidog-yne incognita[J].China Fruits,2006(4):39-41.叶航,简恒,朱立新,王雯君,贾克功.4种桃砧木对南方根结线虫抗性研究[J].中国果树,2006(4):39-41.
• [3]WU Yan-jun,ZHANG Shang-long,XIE Ming,CHEN Jun-wei,QIN Yong-hua,QIN Qiao-ping.Plantlets regeneration from imma-ture embryos and cotyledons of peach[J].Department of Horticulture,2005,41(5):45-50.吴延军,张上隆,谢鸣,陈俊伟,秦永华,秦巧平.桃幼胚及幼胚子叶再生的研究[J].林业科学,2005,41(5):45-50.
• [4]POOLER M R,SCORZA R.Regeneration of peach[Prunus persica(L.)Batsch]rootstock cultivars from cotyledons of mature stored seed[J].Horticulture Science,1995,30(2):355-356.
• [5]SRINIVASAN C,SCORZA R.The influence of genotype on the in-duction of somatic embryos from in vitro cultured zygotic embryos and adventitious shoot regeneration from cotyledons of peach and nectarine[J].Acta Horticulturae,2007,738:691-696.
• [6]GENTILE A,MONTICELLI S,DAMIANO C.Adventitious shoot re-generation in peach(Prunus persica L.Batsch)[J].Plant Cell Re-ports,2002,20:1011-1016.
• [7]YAN Cheng-pu,WANG Zhi-qiang,NIU Liang.Establishment of the system of high efficient plantlet regeneration from different geno-types of peach[C].Inaugural Symposim of China Horticulture,Peach Branch,Zheng zhou,2007.闫承璞,王志强,牛良.不同基因型桃高效植株再生体系的建立[C].中国园艺学会桃分会成立大会暨学术研讨会,郑州,2007.
• [8]ZHOU H C,M,ZHAO X,FAN X C,GUO A G.Plant regeneration from in vitro leaves of the peach rootstock‘Nemaguard’(Prunus persica×P.davidiana)[J].Plant Cell Tissue and Organ Culture,2010,101:79-87.
• [9]P魪REZ-JIM魪NEZ M,CARRILLO-NAVARRO A,COS-TERRER J.Regeneration of peach(Prunus persica L.Batsch)cultivars andPrunus persica×Prunus dulcis rootstocks via organogenesis[J].Plant Cell Tissue and Organ Culture,2012,108:55-62.
• [10]LIU Hang-kong,HAN Ming-yu,YU Ting,ZHAO Cai-ping.Fac-tors affecting embryonic callus from leaves of early season nectarine cultivars[J].Journal of Fruit Science,2006,23(3):370-374.刘航空,韩明玉,禹婷,赵彩平.影响油桃叶片产生胚性愈伤组织的因素[J].果树学报,2006,23(3):370-374.
• [11]CONG Fang,HAN Ming-yu,ZHAO Cai-ping,WAN Chun-yan,LIU Hang-kong.Adventitious bud regeneration in vitro from leaves of peach cultivars[J].Journal of Fruit Science,2009,26(5):614-618.丛芳,韩明玉,赵彩平,万春雁,刘航空.桃叶片再生不定芽的研究[J].果树学报,2009,26(5):614-618.
• [12]LIU Jie-jun,ZHENG Jun-qiang,LUO Xiao-yu,LU Yuan-ping,CHEN Gui-xin,LAI Zhong-xiong.Induction and maintenance ofthe calli for the embryos of the early peach[J].Subtropical Agricul-ture Research,2005,1(4):21-24.刘杰俊,郑君强,罗筱玉,陆远平,陈桂信,赖钟雄.早熟桃胚愈伤组织的诱导与保持[J].亚热带农业研究,2005,1(4):21-24.
• [13]SUN Yuan-wei,LI Guo-guo,YAO Yan-xing,LI Guo-huai.Em-bryonic callus induction and plantlet regeneration in Nubiana(Prunus salicina)[J].Journal of Fruit Science,2011,28(1):42-45.孙源蔚,李果果,姚延兴,李国怀.中国李Nubiana胚性愈伤组织诱导及植株再生[J].果树学报,2011,28(1):42-45.
• [14]SHI Xiao-xin,DU Guo-qiang,MA Bao-kun,WANG Hui-ying,LI Ding.Caiils induction and adventitious bud regeneration from leaves of rootstock of sweet cherry in vitro[J].Journal of Fruit Science,2006,23(4):538-541.师校欣,杜国强,马宝焜,王惠英,李玎.甜樱桃砧木离体叶片愈伤组织诱导及不定芽再生[J].果树学报,2006,23(4):538-541.