基于SSR标记的观赏桃亲缘关系分析SSR analysis on the genetic relationship of ornamental peach germplasm resources
陈霁;马瑞娟;沈志军;蔡志翔;俞明亮;
摘要(Abstract):
观赏桃种质资源丰富,遗传背景复杂,使用SSR标记进行鉴定评价,以期为观赏桃亲缘关系研究提供借鉴。以34份观赏桃品种、7份桃的近缘种和1份毛桃共42份种质为试材,利用16对位于李属植物参考图谱上的SSR标记进行观赏桃亲缘关系研究。结果表明,筛选出的16对SSR引物共扩增出124个清晰条带,多态性条带百分率为87.99%;16对SSR引物均呈单位点扩增模式,平均每个位点扩增等位基因数为7.75。UPGMA聚类分析结果表明,在相似系数0.82时,大部分观赏桃供试品种聚为2大类:第Ⅰ类有12个品种,全部为碧桃;第Ⅱ类包括9个碧桃品种、5个垂枝桃品种、3个寿星桃品种,且所有垂枝桃品种在聚类图中邻接。其余5个观赏桃品种,菊花桃、黄金美丽、红花柱形桃、白花柱形桃、粉花柱形桃聚于这2大类的外侧,且3个具有柱形特征的品种邻接成一类。
关键词(KeyWords): 观赏桃;SSR;亲缘关系
基金项目(Foundation): 国家自然基金(30871681);; 农业部保种项目(NB2011-2130135-07);; 江苏省科技基础设施建设计划(BM2008008)
作者(Author): 陈霁;马瑞娟;沈志军;蔡志翔;俞明亮;
Email:
DOI: 10.13925/j.cnki.gsxb.2011.04.016
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