桃果实中花青苷的提取、检测及应用A Method for Extracting and Determining Anthocyanin From Peach Fruit and Its Application
胡亚东,贾惠娟,孙崇德,陈青俊,徐昌杰,陈昆松
摘要(Abstract)：
采用0.05%盐酸-甲醇提取液4℃浸取组织24h,可以高效提取桃果实果皮中的花青苷,方法回收率可达94.37%;采用梯度洗脱HPLC法,可以对花青苷进行定性、定量分析,该体系检测限(S/N=3)为0.2μg/g,RSD(n=5)为2.94%,峰面积与浓度在0.0625～0.2500mg/mL范围内线性关系达到极显著水平(r=0.997)。方法简便易行,可用于桃果实等材料的花青苷水平变化分析。
关键词(KeyWords)： 果实;花青苷;高效液相色谱;梯度洗脱;提取;检测
基金项目(Foundation): 国家自然科学基金(30170660);; 浙江省自然科学基金重点(ZD0004)项目资助
作者(Author): 胡亚东,贾惠娟,孙崇德,陈青俊,徐昌杰,陈昆松
参考文献(References)：
- [1]VanLO,Blaricom,SennTL.Anthocyaninpigmentsinfreestonepeachesgrowninthesoutheast[J].ProcAmerSocHortSci,1967,90:541～545.
- [2]JanO,JeanCS.Aresearchnote:AnthocyaninsinfruitsofAroniamelanocarpa(Chokeberry)[J].JFoodSci,1990,53(4):1241～1242.
- [3]LengP,QiJX.EffectofanthocyaninonDavidpeach(Prunusda-vidianaFranch.)underlowtemperaturestress[J].SciHort,2003,97:27～39.
- [4]OhmiyaA.Effectsofauxinongrowthandripeningofmesocarpdiscsofpeachfruit[J].SciHort,2000,84:309～319.
- [5]BerenteB,GracíaDDC,ReichenbcherM,etal.Methoddevelop-mentforthedeterminationofanthocyaninsinredwinesbyhigh-per-formanceliquidchromatographyandclassificationofGermanredwinesbymeansofmultivariatestatisticalmethods[J].JChromatog-raphyA,2000,871:95～103.
- [6]HongV,WrolstadRE.UseofHPLCseparation/photodiodearrayde-tectionforcharacterizationofanthocyanins[J].JAgricFoodChem,1990,38:708～715.
- [7]WangHQ,ArakawaO,MotomuraY.Influenceofmaturityandbag-gingontherelationshipbetweenanthocyaninaccumulationandphenylalanineammonia-lyase(PAL)activityin‘Jonathan’apples[J].PostharvestBiologyandTechnology,2000,19:123～128.a..