杨芩;付燕;王永清;陶炼;邓群仙;范建新;邓仁菊;

• 1:凯里学院环境与生命科学学院
• 2:四川农业大学园艺学院
• 3:黔东南民族职业技术学院

摘要(Abstract)：

【目的】鉴定‘黄密’、‘贵妃’、‘早红’和‘软条白沙’4个枇杷品种的S基因型,为其生产栽培合理选择授粉树及杂交育种亲本选择提供科学依据。【方法】以苹果S基因高度保守区设计兼并引物对4个品种的基因组DNA进行PCR扩增,片段回收、克隆及测序,分别采用Blast软件和Bioedit软件进行同源性检索和结构分析。【结果】从参试的4个品种中共分离了4个S等位基因,分别为S2、S5、S6和S31,其中S31-RNase为新分离的枇杷S-RNase基因,Gen Bank登录号为:KC131133。所克隆获得的4个枇杷S-RNase基因均克隆到4个保守区(C2、C3、RC4和C5)和1个高变区(HV),具有与苹果S基因相同的氨基酸序列结构。【结论】确定了参试4个枇杷品种S基因型分别为:‘贵妃’S2-S6、‘黄密’S2-S5、‘早红’S5-S6、‘软条白沙’S6-S31。

关键词(KeyWords)： 枇杷;自交不亲和性;S基因型;克隆

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金地区项目(31460510);; 凯里学院博士专项课题(项目编号:BS201334);; 贵州省教育厅自然科学项目(项目编号:黔教科20090085);; 四川省“十二五”农作物育种攻关项目(2011NZ0098-8)

作者(Author): 杨芩;付燕;王永清;陶炼;邓群仙;范建新;邓仁菊;

Email:

DOI: 10.13925/j.cnki.gsxb.20140304

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