徐呈祥;徐锡增;马艳萍;尚旭岚;

• 1:南京林业大学森林资源与环境学院
• 2:肇庆学院生命科学学院

摘要(Abstract)：

以金丝小枣、梨枣、大瓜枣和冬枣为试材,设加0、0.10%、0.30%、0.50%和0.70%NaCl共5种土壤含盐量水平,研究了盐胁迫下这4个主栽枣品种耐盐性与质膜H+-ATPase和液泡膜H+-ATPase、H+-PPase活性的关系。对叶片相对含水量、丙二醛含量、相对电解质渗漏率、Fv/Fo、Fv/Fm、净光合速率等生理指标测试结果表明,4个枣品种的耐盐性存在较大差异。尽管4品种根尖和叶片细胞PM-H+-ATPase、TP-H+-ATPase和TP-H+-PPase活性随土壤含盐量升高的变化趋势相似,但同一枣品种的不同H+-ATPase、同一H+-ATPase在不同枣品种中的活性差异明显。盐胁迫下,耐盐性强的金丝小枣根尖细胞中3种H+-酶的活性降低幅度明显小于耐盐性弱的冬枣,且金丝小枣PM-H+-ATPase活性与TP-H+-ATPase活性相当,而盐胁迫下耐盐性弱的冬枣根尖细胞中PM-H+-ATPase活性明显低于TP-H+-ATPase活性,TP-H+-PPase活性随土壤含盐量递增更呈直线性下降,对土壤盐分非常敏感。盐胁迫下,枣品种耐盐性不同,枣叶细胞中3种H+-酶活性的差异也很显著。总体上,耐盐性强的枣品种,质膜和液泡膜质子泵的活性均强,根尖细胞向质外体"排盐"的功能与向共质体的液泡中"固定盐分"的功能均突出,叶片细胞向质外体"排盐"的功能基本保持而向液泡中"固定盐分"的功能显著增强,但耐盐性弱的枣品种盐胁迫下叶片细胞质膜、液泡膜质子泵活性下降或略有增强。

关键词(KeyWords)： 枣;盐胁迫;耐盐性;质膜;液泡膜;质子泵活性

Abstract:

Keywords:

基金项目(Foundation): 江苏省博士后科研计划(0701039B);; 中国博士后科学基金(20070411057)

作者(Author): 徐呈祥;徐锡增;马艳萍;尚旭岚;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.02.003

参考文献(References)：

• [1]SERRANO R.Structure and function of proton translocating ATPasein plasma membranes of plants and fungi[J].Biochim Biophys Acta,1988,947:1-28.
• [2]BRISKIN D P.The plasma membrane H+-ATPase of higher plantcells:biochemistry and transport function[J].Biochim Biophys Acta,1990,1019:95-109.
• [3]MORSOMME P,BOUTRY M.The plant plasma membrane H+-AT-Pase:structure,function and regulation[J].Biochim Biophys Acta,2000,1465:1-16.
• [4]SERRANO R.Structure and function of plasma membrane ATPase[J].Annual Review in Plant Physiology and Molecular Biology,1989,40:61-94.
• [5]ROBERTSON WR,CLARK K,YOUNG J C.An Arabidopsis thalianaplasma membrane proton pump is essential for pollen development[J].Genetics,2004,168:1677-1687.
• [6]LIU You-liang,WANG Liang-ju.Responses to salt stress in plantsand its salt tolerance.In:YU Shu-wen,TANG Zhang-cheng.Plantphysiology and molecular biology[M].Beijing:Science Press,1998:752-769.刘友良,汪良驹.植物对盐胁迫的响应及其耐盐性[M]//余叔文,汤章城主编.植物生理学与分子生物学,北京:科学出版社,1998:752-769.
• [7]NIU X,DAMSZ B,KONONOWICZ A K.NaCl-induced alterationsin both cell structure and tissue-specific plasma membrane H+-AT-Pase gene expression[J].Plant Physiol,1996,111:679-686.
• [8]MANSOUR M M F,SALAMA K H A,AL-MUTAWA M M.Trans-port proteins and slat tolerance in plants[J].Plant Science,2003,164:891-900.
• [9]HASEGAVA P M,BRESSAN R A,ZHU J K.Plant cellular andmolecular reseponses to high salinity[J].Annual reviwe of Plant Biol-ogy and Plant Molecular Biology,2000,51:463-499.
• [10]PEDERSON P L,CALAFOLI E.Ion motive ATPase.I.Ubiquity,propertities and significance to cell function[J].Trends in Biochemi-cal Sciences,1987,12:146-150.
• [11]CHINNUSAMY V,JAGENDORF A,ZHU J K.Understanding andimproving salt tolerance in plants[J].Crop Science,2005,45:437-448.
• [12]ZHANG Wen-hua,LIU You-liang.Relationship between tonoplastH+-ATPase activity,ion uptake and calcium in barley roots underNaCl stress[J].Acta Botanica Sinica,2002,44(6):667-672.章文华,刘友良.NaCl胁迫下大麦根中液泡膜H+-ATPase活性,离子吸收与钙的关系[J].植物学报,2002,44(6):667-672.
• [13]QU Ze-zhou,SUN Yun-wei.On Fruit Species[M].Beijing:Agricul-ture Press,1990:232-243.曲泽洲,孙云蔚主编.果树种类论[M].北京:农业出版社,1990:232-243.
• [14]XU Cheng-xiang,XU Xi-zeng.Eco-economic mechanism,optimummodel and management of jujube-grain intercropping[J].Journal ofJiangsu Forestry Science and Technology,2004,32(1):42-45.徐呈祥,徐锡增.枣粮间作的机制优化模式及管理[J].江苏林业科技,2004,32(1):42-45.
• [15]LI Xin-gang,HUANG Jian,GAO Wen-hai.A study on high-qualityproduction regions of dry Chinese jujuba in China[J].Journal of FruitScience,2005,22(6):620-625.李新岗,黄建,高文海.我国制干枣优生区研究[J].果树学报,2005,22(6):620-625.
• [16]WU Zhi-xin,QI Shu-ting.Effects of soil salt in growth and develop-ment of Ziziphus.jujuba cv.Jinsixiaozao[J].China Fruits,1985(2):13-15.武之新,齐树亭.土壤盐分对金丝小枣生长发育的影响[J].中国果树,1985(2):13-15.
• [17]GUO Yu-xin,YANG Mao-lin,SHAN Yun-hua,SHI Feng-xiang,JIA Yao-hua.Studies on the ecological adaptation and developmentregions of Ziziphus.jujuba cv.Jinsixiaozao[J].China Fruits,1985(1):11-14.郭裕新,杨茂林,单云华,石凤祥,贾耀华.金丝小枣生态适应性及发展区域的调查研究[J].中国果树,1985(1):11-14.
• [18]MA Kai.Studies on salt injury and salt tolerance of eighteen fruitspecies[J].Fruit Science,1997,14(1):1-5.马凯.十八种果树的盐害症状及耐盐性研究[J].果树科学,1997,14(1):1-5.
• [19]XU Cheng-xiang.Studies on the effect and mechanism of silicon al-leviating salt stress to Ziziphus jujuba Mill.[D].Nanjing:NanjingForestry University,2005.徐呈祥.硅缓解金丝小枣盐胁迫的效应与机制研究[D].南京:南京林业大学,2005.
• [20]XU Cheng-xiang,XU Xi-zeng.Effect of Silicon on mineral elementmicrodistribution in leaf blade of Ziziphus jujuba Mill.cv.Jinsixi-aozao under NaCl stress[J].Journal of Fruit Science,2010,27(3):28-32.徐呈祥,徐锡增.Si对NaCl胁迫下金丝小枣叶片中矿质元素微域分布的影响[J].果树学报,2010,27(3):28-32.
• [21]TANG Zhang-cheng.Experimental guideline of modern plant physi-ology[M].Beijing:Science Press,2004:207-210.汤章成主编.现代植物生理学实验指南[M].北京:科学出版社,2004:207-210.
• [22]QIAN Hua,LIU You-liang.Protective effect of Calcium to barleyroot plastmembrane and tonoplast under salt stress[J].Journal ofPlant Physiology Comunication,1985,31(2):102-104.钱骅,刘友良.盐胁迫下钙对大麦根系质膜和液泡膜功能的保护效应[J].植物生理学通讯,1985,31(2):102-104.
• [23]CHEN Qin,LIU You-liang.Effects of H2O2 and.OH and theie scav-engers on vacuolar proton pump activity in barley leaves[J].ActaPhytophsiolgica Sinica,1999,25(3):281-286.陈沁,刘友良.H2O2和.OH及其清除剂对大麦叶片液胞膜微囊质子转运活性的影响[J].植物生理学报,1999,25(3):281-286.
• [24]BRADFORD M.A rapid and sensitive method for the quantitation ofmicrogram quantities of protein utilizing the principle of protein-dyebinding[J].Analytic Biochemestry,1976,72:248-254.
• [25]LUNDE C,DREW D P,JACOBS A K,TESTER M.Exclusion of Na+via Sodium ATPase(PpENA1)Ensures Normal Growth ofPhyscomitrella patens under Moderate Salt Stress[J].Plant Physiolo-gy,2007,144:1786-1796.