罗建军,李辉,耿鹏,胡美英

• 1:华南农业大学天然农药与化学生物学教育部重点实验室

摘要(Abstract)：

【目的】为了鉴定从沙糖橘果实表面分离得到的拮抗放线菌株MY-4,并确定该菌株对果蔬病原菌的拮抗作用,【方法】采用分析MY-4形态学、生理生化特性及16S rDNA序列相似性方法以确定其分类地位,并采用平板对峙培养法和生长速率法测定了其抑菌活性。【结果】结果显示,菌株MY-4为小白链霉菌(Streptomyces albulus Routien),菌株MY-4及其无菌发酵液对供试16种果蔬病原真菌均具有拮抗作用,其中菌株MY-4对香蕉炭疽病菌的拮抗作用最强,平板抑菌带宽度达17.08 mm,无菌发酵液对柑橘绿霉病菌的拮抗作用最强,菌丝生长抑制率高达98.82%,【结论】小白链霉菌菌株MY-4具有活性高、抑菌谱广的特点,有望继续研究开发成新的生防剂。

关键词(KeyWords)： 小白链霉菌;拮抗作用;病原菌;生物防治

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30871660);; 广东省科技计划项目(2008B050100045)

作者(Author): 罗建军,李辉,耿鹏,胡美英

DOI: 10.13925/j.cnki.gsxb.2012.04.006

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