杨转英;胡桂兵;王惠聪;欧阳若;陈厚彬;

• 1:华南农业大学园艺生物技术研究所
• 2:华南农业大学园艺生物技术研究所
• 3:华南农业大学园艺学院
• 4:华南农业大学园艺学院

摘要(Abstract)：

以妃子笑和糯米糍2个主栽荔枝品种不同着色期的果皮为试材,经过大量的对比和筛选,采用改良Bugos法提取果皮中的总RNA,经核酸蛋白测定仪测定和凝胶电泳显示提取的总RNA完整性强,质量好,产率高(最高可达318.7μg/g),可用于RT-PCR等分子生物学实验。进一步的DDRT-PCR分析表明:高分辨力的cDNA片段在500～2 000 bp之间,不同品种、不同着色期的荔枝果皮,基因表达有较大差异。

关键词(KeyWords)： 荔枝;果皮;总RNA;差异显示PCR

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(编号:30200188);; 林业科技支撑计划(编号:2006BAD01A1705);; 农业部“948”滚动项目(编号:2006-G31)

作者(Author): 杨转英;胡桂兵;王惠聪;欧阳若;陈厚彬;

Email:

DOI:

参考文献(References)：

• [1]CHOMCZYNSKI P,SACCHI N.Single step method of RNAisolationby acid guanidinium thiocyanate-phenol-chloroform extraction[J].Anal Biochem,1987,162(1):156-159.
• [2]LOGEMANN J,SCHELL J,WILLMITZER L.Improved method forthe isolation of RNAfromplant tissues[J].Anal Biochem,1987,163(1):16-20.
• [3]GU Hong-ya,QU Li-jia,MING Xiao-tian,PAN Nai-sui,CHENZhang-liang.Plant genes and molecular manipulations[M].Beijing:Peking University Press,1995:77-83.顾红雅,瞿礼嘉,明小天,潘乃穟,陈章良.植物基因与分子操作[M].北京:北京大学出版社,1995:77-83.
• [4]VANDRIESSCHE E S,BECCKMANSS,DEJAEGERE R,KANAREKL.Thiourea:the antioxidant of choice for the purification of proteinsfromphenol-rich plant tissues[J].Anal Biochem,1984,141(1):184-188.
• [5]CHANGS,PURYEAR J,CAIRNEYJ.Asimple and efficient methodfor isolation RNA from pine trees[J].Plant Mol Biol Rep,1993,11:113-116.
• [6]LUSheng-dong.Experimental techniques of modern molecular biol-ogy[M].Beijing:Peking Union Medical College Press,1993:135-149.卢圣栋.现代分子生物学实验技术[M].北京:中国协和医科大学出版社,1993:135-149.
• [7]WANCY,WILKINSTA.Amodified hot borate method significant-lyenhance the yield of high-quality RNA from cotton(Gossypiumhirsutuml)[J].Anal Biochem,1994,223(1):7-12.
• [8]XU Chang-jie,CHEN Kun-song,ZHANG Bo,WANG Qian-jie,YEWei-jia.A study on methods for RNA extraction from citrus tissues[J].Journal of Fruit Science,2004,21(2):136-140.徐昌杰,陈昆松,张波,王钱洁,叶薇佳.柑橘组织RNA提取方法研究[J].果树学报,2004,21(2):136-140.
• [9]LIANGP,PARDEE AB.Differential displayofeukaryotic messenger RNA by means of the polymerase chain reaction[J].Science,1992,257:967-971.
• [10]SAMBROOK J,FRITSCH E F,MANIATIS T,Edited.JIN Dong-yan,LI Meng-feng,Translated.Molecular cloning(2nd ed)[M].Beijing:Science Press,1992:343-344.萨姆布鲁克,弗里奇,曼尼阿蒂斯,著.金冬雁,黎孟枫,译.分子克隆实验指南(第2版)[M].北京:科学出版社,1992:343-344.
• [11]ZHANG Yi-shun,XIANG Xu,FU Jia-rui,HUANG Shang-zhi.Amethod for extraction of total RNAfromlitchi embryo[J].Plant Phys-iology Communications,2004,40(2):226-228.张以顺,向旭,傅家瑞,黄上志.一种提取荔枝胚中总RNA的方法[J].植物生理学通讯,2004,40(2):226-228.
• [12]WANG Jia-bao,XU Bi-yu,DU Zhong-jun,LAI Jian-xun,JIN Zhi-qiang.Extracting total RNA from pericarp of postharvest litchi(Litchi chinensis Sonn.)[M].Journal of Agricultural Biotechnology,2006(6):998-999.王家保,徐碧玉,杜中军,赖建勋,金志强.改良CTAB法从采后荔枝果皮中提取总RNA[J].农业生物技术学报,2006(6):998-999.