不同着色期荔枝果皮总RNA的提取及mRNA差显分析Total RNA extraction and mRNA differential display analysis of litchi pericarps at different coloring stages
杨转英;胡桂兵;王惠聪;欧阳若;陈厚彬;
摘要(Abstract):
以妃子笑和糯米糍2个主栽荔枝品种不同着色期的果皮为试材,经过大量的对比和筛选,采用改良Bugos法提取果皮中的总RNA,经核酸蛋白测定仪测定和凝胶电泳显示提取的总RNA完整性强,质量好,产率高(最高可达318.7μg/g),可用于RT-PCR等分子生物学实验。进一步的DDRT-PCR分析表明:高分辨力的cDNA片段在500~2 000 bp之间,不同品种、不同着色期的荔枝果皮,基因表达有较大差异。
关键词(KeyWords): 荔枝;果皮;总RNA;差异显示PCR
基金项目(Foundation): 国家自然科学基金(编号:30200188);; 林业科技支撑计划(编号:2006BAD01A1705);; 农业部“948”滚动项目(编号:2006-G31)
作者(Author): 杨转英;胡桂兵;王惠聪;欧阳若;陈厚彬;
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参考文献(References):
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