马立娜;惠竹梅;霍珊珊;栾丽英;高翔;赵晓琳;

• 1:西北农林科技大学葡萄酒学院·陕西省葡萄与葡萄酒工程中心

摘要(Abstract)：

【目的】为研究2,4-表油菜素内酯(2,4-epibrassinolide,EBR)和脱落酸(abscisic acid,ABA)处理对葡萄花色苷合成与可溶性固形物含量、果皮苯丙氨酸解氨酶(PAL)和类黄酮糖基转移酶(UFGT)活性以及果实内源ABA含量的影响,探索油菜素内酯调控葡萄果实成熟及花色苷合成的机理。【方法】以酿酒葡萄‘赤霞珠’(Cabernet Sauvignon)和‘烟73’(Yan 73)为试材,在葡萄转色前分别用0.1、0.4、0.8 mg.L-1EBR,1mg.L-1Brz(brassinazole,BR生物合成抑制剂)和200 mg.L-1ABA,均匀喷施于葡萄果实,在葡萄成熟过程中测定葡萄果皮花色苷含量及PAL和UFGT酶活性,同时测定果实ABA和可溶性固形物含量。【结果】在果实着色初期,‘赤霞珠’和‘烟73’葡萄果皮PAL和UFGT活性及果实内源ABA含量均逐渐升高,当果实接近成熟花色苷含量基本稳定时ABA含量开始降低。与对照相比,0.4mg.L-1EBR和200 mg.L-1ABA处理显著增加了果实内源ABA含量,提高了果皮PAL和UFGT活性,促进了果皮花色苷的合成和果实可溶性固形物的积累。0.1 mg.L-1和0.8 mg.L-1EBR处理总体增加了果实内源ABA含量,促进了花色苷的合成和可溶性固形物的积累,并提高了UFGT和PAL酶活性,但比0.4 mg.L-1EBR处理提高的幅度小,且差异显著。1 mg.L-1Brz处理使果实ABA的合成推迟,果实可溶性固形物含量、果皮PAL和UFGT活性以及花色苷含量均低于对照,但差异不显著。【结论】外源EBR和ABA处理促进了葡萄成熟和花色苷合成,在不同浓度EBR处理中,以0.4 mg.L-1处理效果较好;内源ABA可能参与了EBR对葡萄成熟和花色苷合成的调控。

关键词(KeyWords)： 葡萄果皮;花色苷;EBR;ABA;PAL;UFGT

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项资金项目(CARS-30);; 陕西省自然基金项目(2011JM3004);; 西北农林科技大学基本科研业务费专项资金项目(QN2009059)

作者(Author): 马立娜;惠竹梅;霍珊珊;栾丽英;高翔;赵晓琳;

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参考文献(References)：

• [1]SPARVOLI F,MARTIN C,SCIENZA A,GAVAZZI G,TONELLI C.Cloning and molecular analysis of structure gene involved in flanonoid and stilbene siosythesis in grape(Vitis vinifera L.)[J].Plant Molecular Biology,1994,24(5):743-755.
• [2]HE J J,LIU Y X,PAN Q H,CUI X Y,DUAN C Q.Different an-thocyanin profiles of the skin and the pulp of Yan73(Muscat Ham-burg x Alicante Bouschet)grape berries[J].Molecules,2010,15(3):1141-1153.
• [3]LIU Chuang-ping,WANG Jun.Anthocyanin biosynthesis in grapevine[J].Plant Physiology Communication,2008,44(2):363-377.刘闯萍,王军.葡萄花色苷的生物合成[J].植物生理学通讯,2008,44(2):363-377.
• [4]JEONG S T,GOTO-YAMAMOTO N,KOBAYASHI S,ESAKA A.Effects of plant hormones and shading on the accumulation of antho-cyanins and the expression of anthocyanin biosynthetic genes in grape berry skins[J].Plant Science,2004,167(2):247-252.
• [5]KOBAYASHI S,ISHIMARU M,DING C K,YAKUSHIJI H,GOTO N.Comparison of UDP-glucose:flavonoid3-O-glucosyltransferase(UFGT)gene sequences between white grapes(Vitis vinifera)and their sports with red skin[J].Plant Science,2001,160(3):543-550.
• [6]BOSS P K,DAVIES C,ROBINSON S P.Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L.cv.Shi-raz grape berries and the implications for pathway regulation[J].Plant Physiology,1996,111(4):1059-1066.
• [7]BOSS P K,DAVIES C,ROBINSON S P.Expression of anthocyanin biosynthesis pathway genes in red and white grapes[J].Plant Molec-ular Biology,1996,32(3):565-569.
• [8]COOMBE B G,HALE C R.The hormone content of ripening grape berries and the effects of growth substance treatments[J].Plant Phys-iology,1973,51(4):629-634.
• [9]HUO Shan-shan,XI Zhu-mei,MA Li-na,LUAN Li-ying.Effect of plant growth regulator on the quality of Cabernet sauvignon grape[J].Journal of Northwest Agriculture and Forestry University:Natural Science Edition,2012,40(1):183-189.霍珊珊,惠竹梅,马立娜,栾丽英.植物生长调节剂对赤霞珠葡萄果实品质的影响[J].西北农林科技大学学报:自然科学版,2012,40(1):183-189.
• [10]MA Huan-pu,CHEN Jing,LIU Zhi-min,LIANG Bao-yan.The ef-fects of natural brassinolide and PDJ on the qualities and maturity of grape fruit[J].Northern Horticulture,2004(4):8-9.马焕普,陈静,刘志民,梁宝岩.天然芸苔素和茉莉酸酯对葡萄果实品质及成熟期的影响[J].北方果树,2004(4):8-9.
• [11]SYMONS G M,DAVIES C,SHAVRUKOV Y,DRY I B,REID J B,THOMAS M R.Grapes on steroids.Brassinosteroids are involved in grape berry ripening[J].Plant Physiology,2006,140(1):150-158.
• [12]CHERVIN C,EL-KEREAMY A,ROUSTAN J P,LATCHE A,LAMO J,BOUZAYEN M.Ethylene seems required for the berry de-velopment and ripening in grape,a non-climacteric fruit[J].Plant Sci-ence,2004,167(6):1301-1305.
• [13]EL-KEREAMY A,CHERVIN C,ROUSTAN J P,CHEYNIER V,SOUQUET J M,MOUTOUNET M,RAYNAL J,FORD C,LATHE A,PETH J C,BOUZAYEN M.Exogenous ethylene stimulates the long-term expression of genes related to anthocyanin biosynthesis in grape berries[J].Physiologia Plantarum,2003,119(2):175-182.
• [14]CHU Zhao-qing,LI Li,SONG Li,XUE Hong-wei.Advances on brassinosteroid biosynthesis and functions[J].Chinese Bulletin of Botany,2006,23(5):543-555.储昭庆,李李,宋丽,薛红卫.油菜素内酯生物合成与功能的研究进展[J].植物学通报,2006,23(5):543-555.
• [15]TIPA-UMOHON A,ROUSTAN J P,CHERVIN C.The stimulation by ethylene of the UDP glucose-flavonoid3-O-glucosyltransferase(UFGT)in grape tissues is independent from the MybA transcription factors[J].Vitis,2007,46(4):210-211.
• [16]DAVIES C,BOSS P K,ROBINSON S P.Treatment of grape berries,a nonclimacteric fruit with a synthetic auxin,retards ripening and alters the expression of developmentally regulated genes[J].Plant Physiology,1997,115(3):1155-1161.
• [17]GAGNE S,CLUZET S,MERILLON J M,GENY L.ABA initiates anthocyanin production in grape cell cultures[J].Journal of Plant Growth Regulation,2011,30(1):1-10.
• [18]VARDHINI B V,RAO S S.Acceleration of ripening of tomato peri-carp discs by brassinosteroids[J].Phytochemistry,2002,61(7):843-847.
• [19]MONTOYA T,NOMURA T,YOKOTA T,HARRISON K,JONES J G D,KANETA T,KAMIYA Y,SZEKERES M,BISHOP G J.Pat-terns of dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit devel-opment[J].Plant Journal,2005,42(2):262-269.
• [20]XIA Guo-hai,ZHANG Da-peng,JIA Wen-suo.The effects of IAA,GA and ABA on14C sucrose input and metabolism in the grapes[J].Acta Horticulturae Sinica,2000,27(1):6-10.夏国海,张大鹏,贾文锁.IAA,GA和ABA对葡萄果实14C蔗糖输入与代谢的调控[J].园艺学报,2000,27(1):6-10.
• [21]DAVIES C,BTTCHER C.Hormonal control of grape berry ripening[M].In:Roubelakis-Angelakis K A Grapevine Molecular Physiology and Biotechnology,Dordrecht:Springer,2009:229-261.
• [22]ROSS J J,WESTON D E,DAVIDSON S E,REID J B.Plant hormone interactions:how complex are they[J].Physiologia Plantarum,2011,141(4):299-309.
• [23]ZHANG Zhao-qi,PANG Xue-jia,DUAN Xue-wu.Change of an-thocyanin content and its determination during lychee pericarp browning[J].Journal of South China Agricultural University,2002,23(1):16-19.张昭其,庞学甲,段学武.荔枝果皮褐变过程中花色素苷含量的变化及测定[J].华南农业大学学报,2002,23(1):16-19.
• [24]LISTER C E,LANCASTER J E,WALKER J R L.Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars[J].Journal of the Science of Food and Agricul-ture,1996,71(3):313-320.
• [25]CHEN Kun-song,XU Chang-jie,LI Fang,CHEN Qing-jun,ZHANG Shang-long.An improved HPLC method for analyzing en-dogenous IAA and ABA in fruit tissues[J].Journal of Fruit Science,2003,20(1):4-7.陈昆松,徐昌杰,李方,陈青俊,张上隆.HPLC法检测果实组织中内源IAA、ABA方法的改进[J].果树学报,2003,20(1):4-7.
• [26]WHEELER S,LOVEYS B,FORD C,DAVIES C.The relationship between the expression of abscisic acid biosynthesis genes,accumu-lation of abscisic acid and the promotion of Vitis vinifera L.berry ripening by abscisic acid[J].Australian Journal of Grape and Wine Research,2009,15(3):195-204.
• [27]PEPP M C,WALKER M A,FIDELIBUS M W.Application of ab-scisic acid rapidly upregulated UFGT gene expression and improved color of grape berries[J].Vitis,2008,47(1):11-14.
• [28]KOYAMA K,SADAMASTU K,GOTO-YAMAMOTO N.Abscisic acid stimulated ripening and gene expression in berry skins of the Cabernet Sauvignon grape[J].Functional&Integrative Genomics,2010,10(3):367-381.
• [29]PAN Q H,LI M J,PENG C C,ZHANG N.ZOU X,ZOU K Q,WANG X L,YU X C,WANG X F,ZHANG D P.Abscisic acid ac-tivates acid invertases in developing grape berry[J].Physiologia Plan-tarum,2005,125(2):157-170.
• [30]GAMBETTA G A,MATTHEWS M A,SHAGHASIT H,MCELRONE A J,CASTELLARIN S D.Sugar and abscisic acid signaling orthologs are activated at the onset of ripening in grape[J].Planta,2010,232(1):219-234.
• [31]WANG Yan-shu,LI Chun-li,JIA Hai-feng,QIN Ling,GAO Dong-sheng,SHEN Yuan-yue.Studies on transcriptional level of VvNCED1and VvBG1,two key genes related to ABA accumulation around the veraison of grape berry ripening[J].Acta Horticulturae Sinica,2010,37(5):1-4.王延书,李春丽,贾海锋,秦岭,高东升,沈元月.葡萄果实始熟前后ABA积累关键酶基因VvNCED1和VvBG1转录水平的研究[J].园艺学报,2010,37(5):1-4.
• [32]KUREPIN L V,QADERI M M,BACK T G,REID D M,PHARIS R P.A rapid effect of applied brassinolide on abscisic acid concentra-tions in Brassica napus leaf tissue subjected to short-term heat stress[J].Plant Growth Regulation,2008,55(3):165-167.
• [33]YUAN G F,JIA C G,LI Z,SUN B,ZHANG L P,LIU N,WANG Q M.Effect of brassinosteroids on drought resistance and abscisic acid concentration in tomato under water stress[J].Scientia Horticul-turae,2010,126(2):103-108.
• [34]HAUBRICK L L,TORSETHAUGEN G,ASSMANN S M.Effect of brassinolide,alone and in concert with abscisic acid,on control of stomatal aperture and potassium currents of Vicia faba guard cell protoplasts[J].Physiologia Plantarum,2006,128(1):134-143.