韩振诚;潘学军;安华明;张文娥;

• 1:贵州大学·贵州省果树工程技术研究中心

摘要(Abstract)：

【目的】探明贵州柿种质资源遗传多样性及亲缘关系状况,为其保护与利用提供理论依据。【方法】利用SRAP分子标记技术对贵州83份柿种质资源的遗传多样性及亲缘关系进行分析。【结果】15对SRAP引物一共扩增出118个DNA位点,其中108个位点具有多态性,占总位点的91.53%,等位基因数(Na)为(1.915 3±0.279 7),有效等位基因数(Ne)为(1.418 9±0.352 1),遗传多样性指数Nei’s(He)为(0.252 6±0.176 0),平均Shannon信息指数(I)为(0.390 6±0.234 2),地区间基因流Nm为3.702 6。UPGMA聚类分析表明,相似系数在0.740时83份柿种质资源被分为5组,居群之间的相似系数与地理距离和种质植物学性状间呈一定的相关性。【结论】贵州柿资源存在丰富的多样性,人为因素导致地区间存在较强的基因流,5个地区间柿种质资源遗传多样性依次为:黔中>黔西>黔南>黔北>黔东。SRAP标记可有效揭示贵州柿种质资源的遗传多样性、分化程度和亲缘关系,研究结果对贵州柿种质资源的保护利用和遗传改良有重要意义。

关键词(KeyWords)： 柿;SRAP;贵州;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技攻关项目“贵州喀斯特山区特异果树种质资源发掘与利用”[黔科合农G字(2009)4003]

作者(Authors): 韩振诚;潘学军;安华明;张文娥;

DOI: 10.13925/j.cnki.gsxb.20150171

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