环剥和脱落酸处理对‘夏黑’葡萄果实着色及相关基因表达的影响Effect of girdling and ABA treatment on fruit quality and peel coloring of ‘Summer Black' grape
任俊鹏;李小红;董瑞奇;宋新新;顾克余;沈月芳;陶建敏;
摘要(Abstract):
【目的】分析环剥和脱落酸(ABA)处理对‘夏黑’果实品质、着色的效果以及对花青苷合成相关重要基因的表达。【方法】在‘夏黑’葡萄着色初期,对主枝进行环剥以及用ABA溶液浸果和喷叶处理。【结果】环剥和ABA处理均可以显著提高果实中可溶性固形物的含量,降低可滴定酸含量,增加果皮花青苷含量,并且明显改善果实着色程度,使果皮颜色加深。其中,环剥对品质的提高最显著,ABA浸果最有利于果皮着色;环剥和ABA处理后果面光泽明亮度L值和颜色组分b值,以及颜色指数h值均下降。300 mg·L-1和500 mg·L-1ABA浸果对果实品质和果皮颜色差异影响不大;500 mg·L-1ABA喷叶对果实品质和果皮颜色指标影响大于300 mg·L-1ABA。ABA浸果处理还明显促进了UFGT、MYBA1和MYBA2的表达,MYBA1和MYBA2于ABA浸果处理后的第2周表达水平明显上调,相对表达量达到最高值。UFGT的表达水平与花青苷的积累趋势具有一致性,各处理均使UFGT的相对表达高峰提前出现,特别是ABA浸果处理后,第2周前后即出现表达最高峰。【结论】环剥和ABA处理均提高了‘夏黑’葡萄果实品质及果皮颜色。
关键词(KeyWords): ‘夏黑’葡萄;环剥;脱落酸;品质;着色
基金项目(Foundation): 国家葡萄产业技术体系(CARS-30);; 江苏省科技厅科技支撑计划(BE2010444);江苏省科技厅科技支撑计划(BE2012453);; 江苏省农业资源开局(2013kj-68);; 江苏省农业三项工程项目SX(2011)236;; 江苏省农业科技自主创新项目(CX(12)3005)
作者(Author): 任俊鹏;李小红;董瑞奇;宋新新;顾克余;沈月芳;陶建敏;
Email:
DOI: 10.13925/j.cnki.gsxb.2013.06.019
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