任俊鹏;李小红;董瑞奇;宋新新;顾克余;沈月芳;陶建敏;

• 1:南京农业大学园艺学院
• 2:江苏丘陵地区镇江农业科学研究所
• 3:河南省农业科学院园艺研究所
• 4:江苏沿海地区农业科学研究所

摘要(Abstract)：

【目的】分析环剥和脱落酸(ABA)处理对‘夏黑’果实品质、着色的效果以及对花青苷合成相关重要基因的表达。【方法】在‘夏黑’葡萄着色初期,对主枝进行环剥以及用ABA溶液浸果和喷叶处理。【结果】环剥和ABA处理均可以显著提高果实中可溶性固形物的含量,降低可滴定酸含量,增加果皮花青苷含量,并且明显改善果实着色程度,使果皮颜色加深。其中,环剥对品质的提高最显著,ABA浸果最有利于果皮着色;环剥和ABA处理后果面光泽明亮度L值和颜色组分b值,以及颜色指数h值均下降。300 mg·L-1和500 mg·L-1ABA浸果对果实品质和果皮颜色差异影响不大;500 mg·L-1ABA喷叶对果实品质和果皮颜色指标影响大于300 mg·L-1ABA。ABA浸果处理还明显促进了UFGT、MYBA1和MYBA2的表达,MYBA1和MYBA2于ABA浸果处理后的第2周表达水平明显上调,相对表达量达到最高值。UFGT的表达水平与花青苷的积累趋势具有一致性,各处理均使UFGT的相对表达高峰提前出现,特别是ABA浸果处理后,第2周前后即出现表达最高峰。【结论】环剥和ABA处理均提高了‘夏黑’葡萄果实品质及果皮颜色。

关键词(KeyWords)： ‘夏黑’葡萄;环剥;脱落酸;品质;着色

Abstract:

Keywords:

基金项目(Foundation): 国家葡萄产业技术体系(CARS-30);; 江苏省科技厅科技支撑计划(BE2010444);江苏省科技厅科技支撑计划(BE2012453);; 江苏省农业资源开局(2013kj-68);; 江苏省农业三项工程项目SX(2011)236;; 江苏省农业科技自主创新项目(CX(12)3005)

作者(Author): 任俊鹏;李小红;董瑞奇;宋新新;顾克余;沈月芳;陶建敏;

Email:

DOI:

参考文献(References)：

• [1]SHIRAISHI S,WATANABE Y.Anthocyanin pigment in the grape skin of several red vitis vinifera cultivars bred in Japan[J].J Fac Agric Kyushu Univ,1991(36):63-67.
• [2]FANG Jing-gui,WANG Tao,YANG Guang.The production profile of greenhouse grape of the city of Wenling,Zhejiang Province[J].SinoOverseas Grapevine&Wine,2009(1):39-43.房经贵,王涛,杨光.浙江温岭市大棚葡萄生产简介[J].中外葡萄与葡萄酒,2009(1):39-43.
• [3]GUO Lei,WANG Tao,YUE Lin-xu,FANG Jing-gui,CHEN Ji-lin,SONG Chang-nian,LENG Xiang-peng.Influence of main-branchgirdling on berry coloring and Expression of some related genes in‘Fujiminori’Grapevine[J].Acta Horticulturae Sinica,2012,39(3):409-416.郭磊,王涛,岳林旭,房经贵,陈济林,宋长年,冷翔鹏.藤稔葡萄主枝环剥对果实着色及相关基因表达的影响[J].园艺学报,2012,39(3):409-416.
• [4]WU Jun,ZHONG Jia-huang,XU Kai,WEI Qin-ping,WEI Zhenlin.Effect of girdling and GA3on fruit development and quality of‘Fujiminori’Grape[J].Journal of Anhui Agricultural Sciences,2001,29(2):219-222.吴俊,钟家煌,徐凯,魏钦平,魏振林.环剥和赤霉素对藤稔葡萄果实生长发育和品质的影响[J].安徽农业科学,2001,29(2):219-222.
• [5]JEONG S T,GOTO-YAMAMOTO N,KOBAYASHI S,ESAKA M.Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins[J].Plant Sciences,2004,167(2):247-252.
• [6]SU Shu-chai.The Studies progress on grapes coloring[J].Viticulture and Winemaking,1994(2):1-4.苏淑钗.葡萄着色问题研究进展[J].葡萄栽培与酿酒,1994(2):1-4.
• [7]ZHANG Zhao-qi,PANG Xue-qun,DUAN Xue-wu,JI Zuo-liang.Change of anthocyanin content and it’s determination during lychee pericarp browning[J].Journal of South China Agricultural University,2002,23(1):16-19.张昭其,宠学群,段学武,季作梁.荔枝果皮褐变过程中花色素苷含量的变化及测定[J].华南农业大学学报,2002,23(1):16-19.
• [8]LI Sheng-hai,GAO Yan,MA Jin-yun,HE Zong-jin,YANG Runde,MA Yi.The initial research of girdling technology on Kyoho grapevine series[J].Tianjin Agirculture and Forestry Science,2003(3):14-15.李胜海,高燕,马金云,贺宗津,杨润德,马翼.巨峰系葡萄环剥技术初探[J].天津农林科技,2003(3):14-15.
• [9]PAN Q H,LI M J,PENG C C,ZHANG N,ZOU X,ZOU K Q,WANG X L,YU X C,WANG X F,ZHANG D P.Abscisic acid activates acid invertases in developing grape berry[J].Physiologia Plantarum,2005,125(2):157-170.
• [10]GAMBETTA G A,MATTHEWS M A,SHAGHASIT H,MCELRONE A J,CASTELLARIN S D.Sugar and abscisic acid signaling orthologs are activated at the onset of ripening in grape[J].Planta,2010,232(1):219-234.
• [11]CHEN Jin-yong,GU Hong,ZHAO Chang-zhu,ZHANG Wei-yuan,WEI Shi-zhong.ABA promote the Kyoho grape coloring and mature test briefing[J].Sino-Overseas Grapevine&Wine,2010(1):43-44.陈锦永,顾红,赵长竹,张威远,魏世忠.ABA促进巨峰葡萄着色和成熟试验简报[J].中外葡萄与葡萄酒,2010(1):43-44.
• [12]JU Zhi-guo.The anthocyanin synthesis and peel coloring of apple[J].Journal of Fruit Science,1991,8(3):43-47.鞠志国.花青苷合成与苹果果皮着色[J].果树科学,1991,8(3):43-47.
• [13]CHEN Jin-yong,FANG Jin-bao,GU Hong,ZHANG Wei-yuan,WEI Shi-zhong.Influence of girdling and gibberellic acid application on the fruit characteristics of Red Globe grape cultivar[J].Journal of Fruit Science,2005,22(6):610-614.陈锦永,方金豹,顾红,张威远,魏世忠.环剥和GA处理对红地球葡萄果实性状的影响[J].果树学报,2005,22(6):610-614.
• [14]GUO Lei,CHEN Ji-lin,SONG Chang-nian,FANG Jing-gui.Effects of girdling time on berry qualities and coloring of Fujiminori’s berry in greenhouse[J].Sino-Overseas Grapevine&Wine,2011(11):10-14.郭磊,陈济林,宋长年,房经贵.不同时期环剥对大棚藤稔葡萄浆果品质及着色过程的影响[J].中外葡萄与葡萄酒,2011(11):10-14.
• [15]SONG Zhe,LI Tian-zhong,XU Gui-xuan.Studies on the relationship among the anthocyanin sugar and related enzymes activity during the coloring stage of‘Fuji’apple[J].Chinese Agricultural Science Bulletin,2008,24(4):255-260.宋哲,李天忠,徐贵轩.“富士”苹果着色期果皮花青苷与果实糖份及相关酶活性变化的关系[J].中国农学通报,2008,24(4):255-260.
• [16]MORI T,SAKURAI M.Effects of riboflavin and increased sucrose on anthocyanin production in suspended strawberry cell cultures[J].Plant Science,1995,110:147-153.
• [17]HU Chun-xia,WANG Li.Effect of ABA and BR on anthocyanin and soluble sugar of grape seeds[J].Journal of Anshan Normal University,2009,11(2):42-44.胡春霞,王丽.ABA和BR对葡萄果实花色素苷和可溶性糖含量的影响[J].鞍山师范学院学报,2009,11(2):42-44.
• [18]MA Li-na,HUI Zhu-mei,HUO Shan-shan,LUAN Li-ying,GAO Xiang,ZHAO Xiao-lin.Studies on the regulation of anthocyanin biosynthesis in grape berry by brassinosteroid and abscisic acid[J].Journal of Fruit Science,2012,29(5):830-836.马立娜,惠竹梅,霍珊珊,栾丽英,高翔,赵晓琳.油菜素内酯和脱落酸调控葡萄果实花色苷合成的研究[J].果树学报,2012,29(5):830-836.
• [19]LI Hong-wei,FENG Shuang-qing.The regulation of abscisic acid and ethylene on the ripening of‘Winter Jujube’[J].Food Science,2003,24(2):147-150.李红卫,冯双庆.ABA和乙烯对冬枣果实成熟衰老的调控[J].食品科学,2003,24(2):147-150.
• [20]BOSS P K,DAVIES C,ROBINSON S P.Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L.cv Shiraz grape berries and the implications for pathway regulation[J].Plant Physiol,1996(111):1059-1066.
• [21]KOBAYASHI S,GOTO-YAMAMOTO N,HIROCHIKA H.Retrotransposon-induced mutations in grape skin color[J].Science,2004(304):982.
• [22]CHEN Kun-song,LI Fang,ZHANG Shang-long,GAVIN S R.Role of abscisic acid and indole-3-acetic acid in Kiwifruit ripening[J].Acta Horticulturae Sinica,1999,26(2):81-86.陈昆松,李方,张上隆,Gavin S.Ross.ABA和IAA对猕猴桃果实成熟进程的调控[J].园艺学报,1999,26(2):81-86.
• [23]CHENG Jian-hui,LEI Ming,YANG Fu-chen,WU Yong-hua,WU Jiang.Anthocyanin accumulation and UFGT gene expression analysis by RT-PCR from cultivars belonging to Vitis vinifera[J].Journal of Fruit Science,2009,26(6):808-812.程建徽,雷鸣,杨夫臣,吴永华,吴江.欧亚种葡萄花色素苷的积累及UFGT基因的RT-PCR表达分析[J].果树学报,2009,26(6):808-812.
• [24]LIU Chuang-ping,WANG Jun.Anthocyanin biosynthesis in grapevine[J].Plant Physiology Communications,2008,44(2):363-377.刘闯平,王军.葡萄花色苷的生物合成[J].植物生理学通讯,2008,44(2):363-377.
• [25]AZUMA A,KOBAYASHI S,GOTO-YAMAMOTO N,SHIRAISHI M,MITANI N,YAKUSHIJI H,KOSHITA Y.Color recovery in berries of grape(Vitis vinifera L.)‘Benitaka’,a bud sport of‘Italia’,is caused by a novel allele at the VvmybA1 locus[J].Plant Science,2009,176(4):470-478.