平欧杂交榛的组织培养Tissue culture of hybrid hazelnut
刘家宁;高遐虹;秦岭;
摘要(Abstract)：
为了探索我国平欧杂交榛离体培养和快繁技术,以5个平欧杂交榛品种(系)的幼嫩茎段为外植体,研究其组织培养及快速繁殖技术,结果表明,(1)5月中旬为茎尖培养取材的较好时期,污染率低、萌芽率高。(2)适宜的基本培养基为DKW培养基。(3)不同基因型间的萌芽率及芽苗生长状态存在极显著差异。(4)增殖阶段较好的培养基为:DKW+TDZ1.5mg/L+IBA0.01mg/L。(5)生根培养较佳培养基为:1/2MS+NAA0.1mg/L,生根率达90%。
关键词(KeyWords)： 杂交榛子;外植株;组织培养
基金项目(Foundation): 北京市农委资助项目(20040212)。
作者(Author): 刘家宁;高遐虹;秦岭;
Email:
DOI:
参考文献(References)：
- [1]LIANG W J,DONG D F(梁维坚,董德芬).Breeding and cultiva-tion of hybrid hazelnut(Corylus heterophylla×C.avellana)(大果榛子育种与栽培)[M].Beijing:China Forest Press,2002:17-20.(in Chinese)
- [2]NAS MN,REEDP E.Ahypothesis for the development of a definedtissue culture medium of higher plants and micropropagation of hazelnuts[J].Scientia Horticulture,2004,101:189-200.
- [3]NAS M N.Inclusion of polyamines in the medium improves shoot elongation in hazelnut(Corylus avellana L.)micropropagation[J].Turkish Journal ofAgriculture and Forestry,2004,28(3):189-194.
- [4]YINCT,SUNMZ,HANAP(尹成涛,孙满芝,韩爱平).Studies on Tissue culture and Rapid Propagation of Corylus avellana L.(欧洲榛子的组培快繁技术研究)[J].Shandong Forest Science and Technology(山东林业科技),2002(5):14-15.(in Chinese)
- [5]YU X,REED BM.A micropropagation system for hazelnut(Corylus species)[J].Hortscience,1995,30:120-123.
- [6]ZHOUJ Y,GUOF X(周俊彦,郭扶兴).Cytokinin activities of ben-zyl derivatives[J].Plant Physiology Communication(植物生理学通讯),1990(4):7-13.(in Chinese)
- [7]ZHANGXH,ZHANGH Y,WUJ(张小红,张红燕,武军).Effect of TDZ on callus inducement and shoot growth in tissue culture of Toona sinensis[J].Journal of Northwest Sci Tech Univ of Agri and For(Nat Sci Ed)(西北农林科技大学学报:自然科学版)2002,30(5):35-39.(in Chinese)
- [8]NAS MN,READ P E.Micropropagation of hybrid hazelnut:medium composition,physical state and iron source affect shoot morphogene-sis,multiplication and explant vitality.In:Proceedings of the Fifth International Congress on Hazelnut[J].Acta Hort,2000,556:251-258.
- [9]AL-KAI H,SALESSES G,MOURAS A.Multiplication in vitro du noisetier(Corylus Avellana L.)[J].Ahronomie,1984(4):399-402.