魏岳荣;黄学林;黄秉智;杨护;邱继水;许林兵;

• 1:广东省农业科学院果树研究所
• 2:中山大学生命科学院教育部基因过程重点实验室
• 3:广东省农业科学院果树研究所
• 4:广东省农业科学院果树研究所

摘要(Abstract)：

以纵切的野生阿宽蕉60d龄未成熟种子为外植体,在MI1愈伤组织诱导培养基(MS大量和微量元素及铁盐+MorelandWetmore维生素+0.1mmol/LKH2PO4+87mmol/L蔗糖+4.5μmol/L2,4-D+1g/LGelrite)中培养60d后开始出现浅黄色松散的胚性愈伤组织,150d后愈伤组织诱导率为(15.11±1.95)%。该类愈伤组织被转移到含18μmol/L2,4-D的MI2培养基中继代60d后,可获得状态均一的理想的胚性愈伤组织。胚性愈伤组织悬浮培养后,通过2个月的筛选和继代培养,可得到均质的胚性细胞悬浮系。理想的胚性愈伤组织在体细胞胚诱导培养基中培养10d后可见到白色半透明体细胞胚的发生,体细胞胚诱导率为7.70×105个/gFW。成熟体细胞胚的萌发率为(33.33±3.37)%,植株再生率为(20.83±1.68)%。

关键词(KeyWords)： 阿宽蕉;胚性愈伤组织;胚性细胞悬浮系;体细胞胚;植株再生

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金资助项目(701126和031995)。

作者(Author): 魏岳荣;黄学林;黄秉智;杨护;邱继水;许林兵;

Email:

DOI:

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