1-MCP对柿果实软化及果胶物质代谢的影响Effect of 1-methylcyclopropene on Persimmon Fruit Ripening and Pectin Metabolism
罗自生
摘要(Abstract):
以扁花柿为试材,研究了20℃下10μL/L的1-甲基环丙烯(1-MCP)处理对柿果实成熟软化及其果胶物质代谢的影响。结果表明:1-MCP处理不仅可推迟柿果实呼吸高峰和乙烯高峰的出现,而且还推迟了柿果实PE、PG和β-Gal酶活性的增加,从而延缓原果胶降解以及水溶性果胶含量增加,保持果肉硬度,延长贮藏期限。
关键词(KeyWords): 柿果实;1-MCP;果胶;细胞壁水解酶
基金项目(Foundation):
作者(Author): 罗自生
参考文献(References):
- 犤1犦高梅,张继澍.亚精胺对柿果采后乙烯生物合成的影响犤J犦.园艺学报,1999,26(6):364~368.
- 犤2犦SislerEC,SerekM.Inhibitorsofethyleneresponsesinplantsatthereceptorlevel:recentdevelopments犤J犦.PhysiolPlant,1997,100:577~582.
- 犤3犦GoldingJB,ShearerD,WyllieSG,etal.Applicationof1-MCPandpropylenetoidentifyethylene-dependentripeningprocessesinmaturebananafruit犤J犦.PostharvestBiolTechnol,1998,14(1):87~98.
- 犤4犦AbdiN,McGlassonWB,HolfordP,etal.Responsesofclimactericandsuppressedclimactericplumstotreatmentwithpropyleneand1-methylcyclopropene犤J犦.PostharvestBiolTechnol,1998,14(1):29~39
- 犤5犦FanX,MattheisJP.Impactof1-methylcyclopropeneandmethyljas-monateonapplevolatileproduction犤J犦.JAgricFoodChem,1999,47:2847~2853.
- 犤6犦LinTP,LiuCC,ChenSW,etal.PurificationandcharacterizationofPEfromFicusawkeotsangMakioachenes犤J犦.PlantPhysiol,1989,91:1445~1453.
- 犤7犦GrossKC.Arapidandsensitivespectrophotometricmethodforassay-ingpolygalacturonaseusing2-cyanoacetamide犤J犦.HortSci,1982,17(6):933~934.
- 犤8犦CarringtonCMS,GreveLC,LabavitchJM.Cellwallmetablisminripeningfruit.Ⅵ.Effectoftheantisensepolygalacturonasegeneoncellwallchangesaccompanyingrieningintransgenictomatoes犤J犦.PlantPhysiol,1993,103:429~434.
- 犤9犦韩雅珊主编.食品化学实验指导犤M犦.北京:中国农业大学出版社,1996.39~41.
- 犤10犦FanX,ArgentaL,MattheisJP.Inhibitionofethyleneactionby1-methylcyclopropeneprolongsstoragelifeofapricots犤J犦.PostharvestBiolTechnol,2000,20(2):135~142.
- 犤11犦FanX,BlankenshipSM,MattheisJP.1-Methylcyclopropeneinhibitsappleripening犤J犦.JAmerSocHortSci,1999,124:690~695.
- 犤12犦NakatsukaA,ShiomiS,KuboY,etal.Expressionandinternalfeed-backregulationofACCsynthaseandACCoxidasegenesinripeningtomatofruit犤J犦.PlantCellPhysiol,1997,38:1103~1110.
- 犤13犦DongL,ZhouHW,LilianS,etal.EthyleneinvolvementinthecoldstoragedisorderofFlavortopnectarine犤J犦.PostharvestBiolTech-nol,23(2):2001,23(2):105~115.
- 犤14犦FengX,ApelbaumA,SislerEC,etal.Controlofethylenerespons-esinavocadofruitwith1-methylcy-clopropene犤J犦.PostharvestBiolTechnol,2000,20(2):143~150.
- 犤15犦FuscgerRL,BennettAB.Roleofcellwallhydrolasesinfruitripen-ing犤J犦.AnnuRevPlantPhysoilPlantMolBiol,1991,42:675~703.
- 犤16犦WakabayashiK,ChunJP,HuberDJ.Extensivesolubilizationanddepolymerizationofcellwallpolysaccharidesduringavocado(Perseaamericana)ripeninginvolvesconcertedactionofpolygalacturonaseandpectinmethylesterase犤J犦.PhysiolPlant,2000,108:345~352.
- 犤17犦WegrzynTF.Pectinesterase,polygalacturonaseandβ-galactosidaseduringsofteningofethylenetreatedkiwifruit犤J犦.HortScience,1992,27(8):900~902.
- 犤18犦KitagawaY,KanayamaY,YamakiS.Isolationofbeta-galactosidasefractionsfromJapanesepear:activityagainstnativecellwallpolysac-charides犤J犦.Physiologia-Plantarum,1995,93(3):545~550.
- 犤19犦Cutillas-IturraldeA,ZarraI,LorencesEP.Metabolismofcellwallpolysaccharidesfrompersimmonfruitpectinsolubilizationduringfruitripeningoccursinapparentabsenceofpolygalacturonaseactivi-ty犤J犦.PhysiologiaPlantarum,1993,89(2):369~375.
- 犤20犦SylviaMB,JohnM.1-Methylcyclopropene:areview犤J犦.PostharvestBiologyandTechnology,2003,28(1):1~25.
- 犤21犦JeongJ,HuberDJ,StevenAS.Influenceof1-methylcyclopropeneonripeningandcell-wallmatrixpolysaccharidesofavocado(Perseaamericana)fruit犤J犦.PostharvestBiolTechnol,2002,25(3):241~256.
- 犤22犦SitritY,BennettAB.RegulationoftomatofruitpolygalacturonasemRNAaccumulationbyethylene:areexamination犤J犦.PlantPhysiol,1998,116:1145~1150.