段续伟;倪杨;张晓明;闫国华;王晶;周宇;张开春;

• 1:北京市林业果树科学研究院·北京市落叶果树工程技术研究中心·农业部都市农业(华北)重点实验室

摘要(Abstract)：

【目的】克隆甜樱桃中可能参与成花途径的MADS基因,分析其基本信息,研究其在不同组织中的表达情况。【方法】以甜樱桃‘萨米特’(‘Summit’)为试材,结合桃基因组分析,克隆得到14个可能参与成花的MADS基因,分别命名为PaSOC1、PaAG、PaAP1、PaAP1-2、PaAP3、PaPI、PaSVP、PaAGL24、PaSEP1、PaSEP2、PaSEP3、PaSEP4、PaSEP5、PaFLC,通过DNAMAN、SMART、protein BLAST和MEGA5等软件分析14个甜樱桃的MADS基因结构、氨基酸结构域及其进化关系,RT-PCR检测其在樱桃根、叶芽、叶、花芽、花、韧皮部中的表达模式。【结果】14个甜樱桃MADS成员大小为612⁷65 bp,均含有典型的MADS结构域和K-box结构域,含有6765 bp,均含有典型的MADS结构域和K-box结构域,含有6⁸个内含子。分属7个亚组,PaSEP1、PaSEP2、PaSEP3、PaSEP4、PaSEP5属于SEP亚组,PaAP1、PaAP1-2属于AP1亚组,PaAG属于AG亚组,PaSOC1属于SOC1亚组,PaAP3和PaPI属于AP3/PI亚组,PaSVP属于SVP亚组,PaAGL24属于AGL24亚组,PaFLC并未聚到FLC亚组中。RT-PCR分析显示,14个MADS基因在花芽或花中均有不同程度的表达,此外,除PaAP3、PaSEP1、PaSEP4及PaSEP5之外,其他几个基因在韧皮部中也有不同程度的表达。【结论】获得的甜樱桃MADS-box基因结构高度保守,参与调控成花及花发育过程。

关键词(KeyWords)： 甜樱桃;MADS-box家族基因;信息学分析;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金(31601726);; 中国博士后科学基金(2016M590063);; 北京市农林科学院科技创新能力建设专项(KJCX20170111)

作者(Authors): 段续伟;倪杨;张晓明;闫国华;王晶;周宇;张开春;

DOI: 10.13925/j.cnki.gsxb.20170204

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