苏立遥;蒋梦琦;黄倏祺;徐小萍;厉雪;陈旭;赖钟雄;林玉玲;

• 1:福建农林大学园艺植物生物工程研究所

摘要(Abstract)：

【目的】探究miR403的分子进化特性及其在龙眼体胚发生早期中的表达模式。【方法】根据龙眼转录组数据库结合miRBase数据库下载得到植物miR403成熟体(miRNA,MicroRNA)和前体(pre-miRNA,Precursor MicroRNA)序列,对其构建系统发育树、序列多重比对、前体二级结构分析;并克隆龙眼miR403 5’末端cDNA序列,比较miR403启动子顺式作用元件。利用qPCR(Quantitative real time-PCR)探究龙眼体胚发生早期miR403和pre-miR403的表达模式。【结果】龙眼及其他17个物种中miR403的36个前体和42成熟体序列的系统发育树以及序列多重比对显示,植物miR403高度保守且主要来源于pre-miR403的3臂,而pre-miR403分歧较大,序列仅在miRNA生成区域保守;不同物种之间miR403成员数量差异不大;pre-miR403最小折叠自由能为-35.70～-55.50 kal·mol~(-1)。克隆获得龙眼primiR403(miR403初级体)5’末端cDNA序列,确认其第一个碱基A为转录起始位点(TSS,transcription start site)。龙眼等5种植物MIR403启动子中具有大量光、激素和逆境胁迫等元件。龙眼早期体胚发生过程中miR403和pre-miR403整体为显著下调趋势。【结论】龙眼miR403与其他植物miR403具有高度的保守性以及物种之间的特异性,miR403和pre-miR403下调表达可能有利于促进龙眼早期体胚发生。

关键词(KeyWords)： 龙眼;microRNA403;进化特性;启动子;体胚发生;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31672127);; 福建省高原学科建设经费(102/71201801101);; 福建农林大学园艺学院优秀硕士学位论文资助基金(2018S02)

作者(Author): 苏立遥;蒋梦琦;黄倏祺;徐小萍;厉雪;陈旭;赖钟雄;林玉玲;

Email:

DOI: 10.13925/j.cnki.gsxb.20190077

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