平邑甜茶后代双胚苗的倍性鉴定及核型分析Ploidy identification and karyotype analysis on the seedlings from di-embryonic seeds of Pingyi Tiancha(Malus hupehensis var.pingyiensis)
王颖;周攀;魏鑫;王玉霞;董文轩;
摘要(Abstract):
为了确定平邑甜茶双胚苗的倍性特征和核型特点,采用压片和流式细胞仪观测的方法对平邑甜茶后代中双胚苗进行染色体倍性观察和核型分析。结果表明:4株供试材料均为三倍体,2n=3x=51,染色体为小型,染色体长度比差异不大;核型组成由中部着丝点染色体(m)和亚中部着丝点染色体(sm)组成;株系8-1、8-2、12-1和12-2的核型公式分别为2n=3x=45m+6sm,2n=3x=48m+3sm,2n=3x=27m+24sm,2n=3x=33m+18sm;染色体相对长度和着丝点指数的变异范围分别为1.21%~2.68%和34.19%~42.37%,1.44%~2.63%和35.59%~44.08%,1.36%~3.02%和32.85%~42.18%,1.43%~2.87%和34.23%~44.02%;核型分别属于1B、1A、2B和1B类型,表现为株系之间的核型不完全相同。本研究为丰富无融合生殖型砧木的育种理论,获得新的苹果无融合生殖型砧木提供有益的借鉴。
关键词(KeyWords): 平邑甜茶;双胚苗;染色体倍性;核型分析
基金项目(Foundation): 国家自然科学基金(30370985)
作者(Author): 王颖;周攀;魏鑫;王玉霞;董文轩;
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