吴建阳;李彩琴;陆旺金;李建国;

• 1:华南农业大学中国荔枝研究中心
• 2:湛江师范学院基础教育学院
• 3:华南农业大学园艺学院广东省果蔬保鲜重点实验室

摘要(Abstract)：

【目的】为了探明荔枝ACC氧化酶(ACO)基因与荔枝幼果脱落的关系,【方法】用RT-PCR和RACE扩增技术相结合的方法进行ACO基因的克隆;在‘黑叶’荔枝花后30 d喷施100 mg·L-1萘乙酸(NAA)来进行荔枝幼果落果的处理;用荧光定量PCR技术分析ACO基因表达与荔枝幼果脱落的关系。【结果】首次从荔枝中分离得到ACO基因,并命名为Lc-ACO1。Lc-ACO1全长为1 231 bp,其中5′非编码区包含89 bp,3′非编码区包含215 bp,开放阅读框包含927 bp,编码309个氨基酸。Lc-ACO1基因含有ACO基因特有的7个保守区和9个不变氨基酸残基。100 mg·L-1萘乙酸(NAA)可以显著促进荔枝幼果的脱落,且处理后相对落果率高峰出现在第10天,而Lc-ACO1基因在离区和幼果中的表达量高峰都出现在第7天,基因表达量高峰比相对落果率高峰早出现3 d。【结论】Lc-ACO1基因可能与荔枝幼果的脱落密切相关。

关键词(KeyWords)： 荔枝;ACC氧化酶;基因;克隆;表达;落果

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31171920);; 国家现代农业产业技术体系(CARS-33-11)

作者(Author): 吴建阳;李彩琴;陆旺金;李建国;

Email:

DOI: 10.13925/j.cnki.gsxb.2013.02.020

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