邢婷婷;杨航宇;王雯染;杨晓慧;王军;

• 1:中国农业大学食品科学与营养工程学院葡萄与葡萄酒研究中心
• 2:农业部葡萄酒加工重点实验室

摘要(Abstract)：

【目的】对14个欧亚种红色酿酒葡萄品种(品系)的花色苷组成和含量进行分析,以探究利用花色苷作为化学指纹来区分品种。【方法】以北京市海淀区中国农业大学上庄实验站的14个欧亚种红色酿酒葡萄品种(品系)为材料,利用高效液相色谱-质谱联用(HPLC-MS)方法检测其果皮中的花色苷组成和含量,对不同葡萄品种中各种花色苷类型进行比较,并基于各单体花色苷含量进行聚类分析和主成分分析。【结果】共检测到21种花色苷,‘西拉’花色苷含量最高;‘黑比诺115’的二甲花翠素-3-葡萄糖苷相对含量最高,且不含酰化类花色苷;‘丹魄’是花翠素-3-葡萄糖苷、甲基花翠素-3-葡萄糖苷相对含量较高的品种。从酰化花色苷的相对含量可以看出,乙酰化和香豆酰化花色苷相对含量高于咖啡酰化类,‘马贝克’‘西拉’酰化花色苷相对含量高于其他品种;‘品丽珠’和‘内比奥罗’的乙酰化花色苷相对含量最高;‘马贝克’的香豆酰化花色苷含量最高;‘蒙特布查诺’的咖啡酰化类花色苷含量最高。聚类分析表明,供试的14个品种(品系)可以被分为2大类,第1大类是‘黑比诺115’,第2大类包括‘马贝克’和其他品种。主成分分析将‘黑比诺115’和‘多塞托’分在第1象限,‘蒙特布查诺’和‘丹魄’分在第2象限,第3象限是‘比诺塔吉’‘内比奥罗’‘西拉’和‘马贝克’,‘品丽珠’和‘美乐’的各个品系都在第4象限。【结论】不同品种单体花色苷所占比例有明显差异,供试的14个葡萄品种(品系)主成分分析结果与聚类分类结果基本一致,花色苷可以作为化学指纹区分14个欧亚种红色酿酒葡萄品种(品系)。

关键词(KeyWords)： 红色酿酒葡萄;花色苷;聚类分析;主成分分析

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(CARS-29);; 北京市科技计划课题(D171100002317001)

作者(Author): 邢婷婷;杨航宇;王雯染;杨晓慧;王军;

Email:

DOI:

参考文献(References)：

• [1]PARK S W,CHANG E J,HA T Y,PARK K H.Antioxidative activity of acylated anthocyanin isolated from fruit and vegetables[J].Preventive Nutrition and Food Science,1997,2(3):191-196.
• [2]孙明霞,王宝增,范海,赵可夫.叶片中的花色素苷及其对植物适应环境的意义[J].植物生理学报,2003,39(6):688-694.SUN Mingxia,WANG Baozeng,FAN Hai,ZHAO Kefu.Anthocyanins of leaves and their enviromental significance in plant stress responses[J].Plant Physiology Journal,2003,39(6):688-694.
• [3]LIANG Z,WU B,FAN P,YANG C,DUAN W,ZHENG X,LIU C,LI S.Anthocyanin composition and content in grape berry skin in Vitis germplasm[J].Food Chemistry,2008,111(4):837-844.
• [4]CARRE?O J,MARTíNEZ A,ALMELA L,FERN?NDEZLóPEZ J A.Proposal of an index for the objective evaluation of the colour of red table grapes[J].Food Research International,1995,28(4):373-377.
• [5]COOPER-DRIVER G A.Contributions of Jeffrey Harborne and co-workers to the study of anthocyanins[J].Phytochemistry,2001,56(3):229-236.
• [6]房经贵,刘崇怀.葡萄分子生物学[M].北京:科学出版社,2014.FANG Jinggui,LIU Chonghuai.Grape molecular biology[M].Beijing:Science Press,2014.
• [7]AGEORGES A,FERNANDEZ L,VIALET S,MERDINOGLU D,TERRIER N,ROMIEU C.Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red colour of grape berries[J].Plant Science,2006,170(2):372-383.
• [8]AROZARENA I,AYESTARáN B,CANTALEGO M,NAVARRO M,VERA M,ABRIL I,CASP A.Anthocyanin composition of Tempranillo,Garnacha and Cabernet Sauvignon grapes from high and low-quality vineyards over two years[J].European Food Research and Technology,2002,214(4):303-309.
• [9]ESTEBAN M A,VILLANUEVA M J,LISSARRAGUE J R.Effect of irrigation on changes in the anthocyanin composition of the skin of cv.Tempranillo(Vitis vinifera L.)grape berries during ripening[J].Journal of the Science of Food and Agriculture,2001,81(81):409-420.
• [10]REVILLA E,GARCíA BENEYTEZ E,CABELLO F.Anthocyanin fingerprint of clones of Tempranillo grapes and wines made with them[J].Australian Journal of Grape and Wine Research,2009,15(1):70-78.
• [11]BOSS P K,DAVIES C,ROBINSON S P.Expression of anthocyanin biosynthesis pathway genes in red and white grapes[J].Plant Molecular Biology,1996,32(3):565-569.
• [12]FERN?NDEZ-LóPEZ J,ALMELA L,MUNOZ J A,HIDALGOB V,CARRE?O J.Dependence between colour and individual anthocyanin content in ripening grapes[J].Food Research International,1998,31(9):667-672.
• [13]MATTIVI F,GUZZON R,VRHOVSEK U,STEFANINI M,VELASCO R.Metabolite profiling of grape:flavonols and anthocyanins[J].Journal of Agricultural and Food Chemistry,2006,54(20):7692-7702.
• [14]YANG J,TIMOTHY E,MARTINSON T E,LIU R H.Phytochemical profiles and antioxidant activities of wine grapes[J].Food Chemistry,2009,116(1):332-339.
• [15]AROZARENA I,CASP A,MARíN R,NAVARRO M.Differentiation of some Spanish wines according to variety and region based on their anthocyanin composition[J].European Food Research and Technology,2000,212(1):108-112.
• [16]POMAR F,NOVO M,MASA A.Varietal differences among the anthocyanin profile of 50 red table grape cultivars studied by high performance liquid chromatography[J].Journal of Chromatography A,2005,1094(1):34-41.
• [17]GONZáLEZ-NEVES G,FRANCO J,BARREIRO L,GILL G,MOUTOUNET M,ALAIN C.Varietal differentiation of Tannat,Cabernet Sauvignon and Merlot grapes and wines according to their anthocyanic composition[J].European Food Research and Technology,2007,225(1):111-117.
• [18]OTTENEDER H,MARX R,ZIMMER M.Analysis of the anthocyanin composition of Cabernet Sauvignon and Portugieser wines provides an objective assessment of the grape varieties[J].Australian Journal of Grape and Wine Research,2008,10(1):3-7.
• [19]MONAGAS M,Nú?EZ V,BARTOLOMéB,GóMEZ-CORDOVéS C.Anthocyanin-derived pigments in Graciano,Tempranillo,and Cabernet Sauvignon wines produced in Spain[J].American Journal of Enology and Viticulture,2003,54(3):163-169.
• [20]Nú?EZ V,MONAGAS M,GóMEZ-CORDOVéS M C,BARTOLOMéB.Vitis vinifera L.cv.Graciano grapes characterized by its anthocyanin profile[J].Postharvest Biology and Technology,2004,31(1):69-79.
• [21]DIMITROVSKA M,BOCEVSKA M,DIMITROVSKI D,MURKOVIC M.Anthocyanin composition of Vranec,Cabernet Sauvignon,Merlot and Pinot Noir grapes as indicator of their varietal differentiation[J].European Food Research and Technology,2011,232(4):591-600.
• [22]HE J J,LIU Y X,PAN Q H,CUI X Y,DUAN C Q.Different anthocyanin profiles of the skin and the pulp of Yan73(Muscat Hamburg×Alicante Bouschet)grape berries[J].Molecules,2010,15(3):1141-1153.
• [23]ORTEGA-REGULES A,ROMERO-CASCALES I,LóPEZ-ROCA J M,ROS-GARCíA J M,GóMEZ-PLAZA E.Anthocyanin fingerprint of grapes:environmental and genetic variations[J].Journal of the Science of Food and Agriculture,2010,86(10):1460-1467.
• [24]李小龙,张振文.不同酿酒葡萄品种果实成熟过程中花色苷含量变化[J].北方园艺,2014(22):13-17.LI Xiaolong,ZHANG Zhenwen.Change of anthocyanins in different Vitis vinifera varieties during ripening[J].Northern Horticulture,2014(22):13-17.
• [25]FIGUEIREDO-GONZáLEZ M,MARTíNEZ-CARBALLO E,CANCHO-GRANDE B,SANTIAGO J L,MARTíNEZ M C,SIMAL-GáNDARA J.Pattern recognition of three Vitis vinifera L.red grapes varieties based on anthocyanin and flavonol profiles,with correlations between their biosynthesis pathways[J].Food Chemistry,2012,130(1):9-19.
• [26]REVILLA E,GARCíA-BENEYTEZ E,LóPEZ J,CABELLO F.Anthocyanin pattern of several red grape cultivars and single-cultivar young wines[J].Acta Alimentaria,2013,42(1):23-35.