梁文杰;谭晓风;乌云塔娜;

• 1:温州科技职业学院
• 2:中南林业科技大学经济林育种与栽培国家林业和草原局重点实验室
• 3:中国林业科学研究院经济林研究开发中心

摘要(Abstract)：

【目的】获得梨S-RNase基因全长序列及其进化、遗传多态性情况。【方法】设计特异性引物扩增梨S42-RNase基因组DNA全长序列,利用RT-PCR结合cDNA末端快速扩增(rapid amplification of cDNA ends,RACE)技术获得其cDNA全长序列。构建梨属植物S-RNase基因编码区和高变区(hyper variable region,HV)内含子的系统发育树。【结果】梨S42-RNase基因编码226个氨基酸,包含由27个氨基酸组成的信号肽,由11、11、6、8、7个氨基酸组成的5个保守区以及HV区插入的335 bp内含子序列。系统进化树显示,梨S-RNase基因编码区和HV区内含子的拓扑结构既相似又有差异。编码区Normalized dN-dS结果显示,除了HV区外还有几个区域存在大量的非同义替换,表明对阳性选择具有很高的敏感性。【结论】梨S-RNase基因编码区和HV区内含子的进化存在一定的关联性,除HV区外还存在其他区域参与梨雌蕊与花粉间特异识别。梨S-RNase基因编码区进化动力源自其编码特异识别蛋白的功能,是平衡选择的结果。HV区的内含子序列表现出很高的长度多态性,进化受到负选择的压力。

关键词(KeyWords)： 梨;S-RNase;基因克隆;进化分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（31000309）

作者(Author): 梁文杰;谭晓风;乌云塔娜;

Email:

DOI: 10.13925/j.cnki.gsxb.20210195

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