姜倩倩;孙晓莉;曹慧;邹岩梅;束怀瑞;

• 1:山东省高校生物化学与分子生物学重点实验室·潍坊学院
• 2:山东省果树研究所
• 3:国家苹果工程技术研究中心

摘要(Abstract)：

【目的】为了探明植物络合素酶参与植物解毒重金属的作用,【方法】以湖北海棠(Malus hupehensis)为试材,克隆其植物络合素合酶基因(MhPCS)编码区序列,并研究该基因的表达特点。【结果】结果表明MhPCS基因编码区全长为1 494 bp,编码497个氨基酸。序列分析表明,MhPCS编码的氨基酸序列由2个典型的植物络合素合酶亚家族结构域组成,具有3个相邻的Cys-Cys元件(331-332、351-352和369-370位氨基酸)和植物络合素合酶蛋白的特征位点(Cys-56、Cys-90/91和Cys-109)。【结论】湖北海棠MhPCS与杜梨PbPCS蛋白同源性最高(94%),属于系统发生树的同一分支。MhPCS基因为组成型表达,各器官表达水平存在差异,其中根的表达量最高;100μmol.L-1CdSO4处理48 h内,MhPCS基因的表达量迅速上升;不同金属离子对其上调表达的诱导能力为:Cd2+>Cu2+>Pb2+。这为揭示重金属胁迫下湖北海棠环境适应的分子机制奠定了基础。

关键词(KeyWords)： 湖北海棠;植物络合素合酶基因(MhPCS);克隆;表达

Abstract:

Keywords:

基金项目(Foundation): 国家现代苹果产业技术体系;; 山东省自然科学基金(Y2007D60);; 潍坊市科技发展计划(20121303);; 潍坊学院博士基金(2012BS18)

作者(Author): 姜倩倩;孙晓莉;曹慧;邹岩梅;束怀瑞;

Email:

DOI: 10.13925/j.cnki.gsxb.2013.03.006

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