农杆菌介导Ferritin基因转化苹果的研究Agrobacterium-mediated transformation of apple with ferritin gene
叶霞,黄晓德,陶建敏,乔玉山,姚泉洪,章镇
摘要(Abstract):
以皇家嘎拉苹果的叶片为转化受体,通过根癌农杆菌介导将铁结合蛋白(Ferritin)基因导入受体中,诱导获得了抗性芽.经PCR检测从抗Km植株中选择到4株阳性植株,进一步经PCR-Southern杂交证实外源基因已整合到4株苹果基因组中,RT-PCR检测表明,Ferritin基因在4株转基因植株中得到了表达.
关键词(KeyWords): 铁结合蛋白;根癌农杆菌;基因转化;苹果
基金项目(Foundation): 国家自然科学基金项目(30370987);; 江苏省应用基础项目(BJ200018)。
作者(Author): 叶霞,黄晓德,陶建敏,乔玉山,姚泉洪,章镇
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