许文天;武红霞;高玉尧;李丽;梁清志;马小卫;姚全胜;詹儒林;王松标;

• 1:中国热带农业科学院南亚热带作物研究所·农业部热带果树生物学重点实验室·国家重要热带作物工程技术研究中心

摘要(Abstract)：

【目的】明确幼胚愈伤诱导和胚抢救最佳时期,为杧果高效体胚发生体系构建及胚抢救技术有效利用提供依据。【方法】以'热农1号'杧果为材料,探讨幼胚不同发育时期和植物生长调节剂对愈伤组织诱导的影响,同时比较了幼胚不同发育时期对幼胚萌发的影响。【结果】授粉后25 d幼胚处于球形胚期,30 d处于心形胚期,35 d处于鱼雷胚期,40 d之后处于子叶胚期。授粉后40 d早期子叶胚愈伤组织诱导率显著高于其他时期;早期子叶胚在含有3.0 mg·L~(~(-1))2,4-D、1.0 mg·L~(~(-1))KT(或0.5 mg·L~(~(-1))KT及0.5 mg·L~(~(-1))ZT)培养基(PM3和PM4)中愈伤组织诱导率最高,为54.4%～62.2%,显著高于其他培养基。授粉后35 d鱼雷胚的萌发率最高,为47.2%,显著高于其他时期。愈伤组织能经体胚发生途径正常成苗,幼胚萌发后也能正常发育成苗。【结论】'热农1号'杧果幼胚愈伤诱导最适宜的时期是早期子叶胚,胚抢救最适宜的时期是鱼雷胚阶段。

关键词(KeyWords)： 杧果;体细胞胚;胚抢救;胚发育时期;细胞学观察

Abstract:

Keywords:

基金项目(Foundation): 海南省自然科学基金(317245);海南省自然科学基金创新研究团队项目(2019CXTD410);; 中央级公益性科研院所基本科研业务专项(1630062016002);; 国家重点研发专项(2017YFD0202107-7)

作者(Author): 许文天;武红霞;高玉尧;李丽;梁清志;马小卫;姚全胜;詹儒林;王松标;

Email:

DOI: 10.13925/j.cnki.gsxb.20190281

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