徐广春;顾中言;罗楚平;徐德进;许小龙;陈志谊;

• 1:江苏省农业科学院植物保护研究所

摘要(Abstract)：

【目的】为了获得枯草芽孢杆菌sf-628的专用助剂,【方法】将sf-628与6种不同的表面活性剂组合按重量比95∶5进行混配,并以菌液中的活菌量为依据进行筛选。同时采用国标GB 5549-90的方法测定sf-628与各个表面活性剂组合混配后稀释液的表面张力,并将其与苹果、梨叶片的临界表面张力进行比较分析。【结果】结果表明,苹果叶片正、反面的临界表面张力值分别为31.78 mN.m-1和33.75 mN.m-1;梨叶片的临界表面张力值分别为39.90mN.m-1和38.02 mN.m-1。活菌量的测定结果表明4种表面活性剂组合对sf-628表现为抑制作用,其余2种表面活性剂组合(Ⅲ和IV)则对其安全。将其与sf-628混配后0～1 000倍稀释液中,表面活性剂组合Ⅲ和IV达到临界胶束浓度,表面活性剂组合Ⅲ稀释液的表面张力大于苹果叶片的临界表面张力,故难以很好地在苹果叶片上润湿展布;表面活性剂组合IV稀释液的表面张力小于苹果、梨叶片的临界表面张力,故可以在苹果、梨叶片上黏着并润湿展布。【结论】表面活性剂组合IV可作为枯草芽孢杆菌sf-628的专用助剂在苹果、梨树上使用。

关键词(KeyWords)： 枯草芽孢杆菌;临界表面张力;表面活性剂;sf-628;苹果;梨

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(200903033);; 江苏省农业科技自主创新资金项目(CX(11)2045)

作者(Authors): 徐广春;顾中言;罗楚平;徐德进;许小龙;陈志谊;

DOI: 10.13925/j.cnki.gsxb.2012.05.002

参考文献(References)：

• [1]YUAN Hui-zhu,YANG Dai-bin,YAN Xiao-jing,ZHANG Lin-na.Pesticide efficient and the way to optimize the spray application[J].Plant protection,2011,37(5):14-20.袁会珠,杨代斌,闫晓静,张琳娜.农药有效利用率与喷雾技术优化[J].植物保护,2011,37(5):14-20.
• [2]GU Zhong-yan,XU Xiao-long,HAN Li-juan.Different surface ten-sion monosultap microemulsion droplets in the rice leaf surface of the behavioral characteristics[J].Chinese Rice Science,2004,18(2):176-180.顾中言,许小龙,韩丽娟.不同表面张力的杀虫单微乳剂药滴在水稻叶面的行为特性[J].中国水稻科学,2004,18(2):176-180.
• [3]GU Zhong-yan.Analysis of the relationship between hydrophilic or hydrophobic property of plant and action of pesticides solution on plants leaves[J].Jiangsu Journal of Agricultural Sciences,2009,25(2):276-281.顾中言.植物的亲水疏水特性与农药药液行为的分析[J].江苏农业学报,2009,25(2):276-281.
• [4]YU Y,ZHU H,FRANTZ J M,READING M E,CHAN K C,OZKAN H E.Evaporation and coverage area of pesticide droplets on hairy and waxy leaves[J].Biosystems Engineering,2009,104:324-334.
• [5]QIU Zhan-kui,YUAN Hui-zhu,LI Yong-ping,JI Ming-shan,HUAN Xiong-ying,FENG Sen.Influence of the surfactant silwet408 on wheat aphids control efficacy using low volume spraying[J].Plant Protection,2006,32(2):34-37.邱占奎,袁会珠,李永平,纪明山,黄雄英,逢森.添加有机硅表面活性剂对低容量喷雾防治小麦蚜虫的影响[J].植物保护,200632(2):34-37.
• [6]WIRTH W,STORP S,JACOBSEN W.Mechanisms controlling leaf retention of agricultural spray solutions[J].Pesticide Science,1991,33(4):411-420.
• [7]TU Yu-qin.Pesticide formulation and dose transfer[J].Chinese Jour-nal of Pesticide Science,1999,1(1):1-6.屠豫钦.农药剂型和制剂与农药的剂量转移[J].农药学学报,1999,1(1):1-6.
• [8]LIU You-zhou,CHANG You-hong,WEI Ben-qiang,LIU Yong-feng,CHEN Zhi-yi.Screening and evaluation of antagonistic bacte-ria against Botryosphaeria berengeriana f.sp.Piricola[J].Journal of Fruit Science,2009,26(3):344-348.刘邮洲,常有宏,魏本强,刘永峰,陈志谊.梨轮纹病拮抗细菌的筛选与评价[J].果树学报,2009,26(3):344-348.
• [9]PANG Hong-yu,HUAN Qin,MA Chen,DU Feng-pei.Studies on relation of droplet contact angel to droplet volume and measuring time[J].Journal of Henan Agricultural Science,2005,12:51-54.庞红宇,黄琴,马琛,杜凤沛.雾滴体积和测量时间与雾滴接触角的关系[J].河南农业科学,2005,12:51-54.
• [10]TU Yu-qin,LI Bing-li.Introduction to application technology of pesticides[M].Beijing:Chemical Industry Press,2006:33-36.屠豫钦,李秉礼.农药应用工艺学导论[M].北京:化学工业出版社,2006:33-36.
• [11]GU Zhong-yan,XU Xiao-long,HAN Li-juan.Study on the measure of the critical surface tension of plants[J].Modern Agrochenicals,2002,2:18-20.顾中言,许小龙,韩丽娟.几种植物临界表面张力值的估测[J].现代农药,2002,2:18-20.
• [12]CHEN Zhi-yi,MIAO Dong-hua.Relationship between the Colo-nization,concentration and spray timing of antagonistic bacteria and sheath blight of rice[J].Jiangsu Journal of Agricultural Sciences,1998,14(1):31-35.陈志谊,苗东华.拮抗细菌的定殖、浓度和喷施期与水稻纹枯病的关系[J].江苏农业学报,1998,14(1):31-35.
• [13]ZHANG Li-li,CHANG You-hong,DING Fang-bing,LIU You-zhou,LIU Chang-he,CHEN Zhi-yi.Inhibition effect of2Bacillus subtilis on Botryosphaeria berengeriana f.sp.piricola in vitro[J].Journal of Fruit Science,2010,27(5):823-827.张丽丽,常有宏,丁芳兵,刘邮洲,刘长河,陈志谊.2株枯草芽孢杆菌对梨轮纹病菌的室内抑制作用研究[J].果树学报,2010,27(5):823-827.
• [14]GU Zhong-yan,TANG Wei-ai,CHEN Zhi-yi,XU Xiao-long,LIU Yong-feng,LIU You-zhou.Influence of surfactants on fungicidal activity and control eficacy against rice sheath bligh of the bio-pes-ticide Wenquning[J].Jiangsu Journal of Agricultural Sciences,2005,21(3):162-166.顾中言,唐为爱,陈志谊,许小龙,刘永峰,刘邮洲.表面活性剂对生物农药纹曲宁抑茵活性和防病效果的影响[J].江苏农业学报,2005,21(3):162-166.
• [15]PHILIP T.The wetting of leaf surfaces[J].Current Opinion in Colloid and Interface Science,2011,16(4):326-334.