郝卫宁;李辉;杨柳;耿鹏;胡美英;

• 1:华南农业大学天然农药与化学生物学教育部重点实验室

摘要(Abstract)：

为明确茶皂素和噻菌灵混配对柑橘青霉和绿霉病菌的抑制作用,采用菌丝生长速率法和共毒系数法测定了茶皂素、噻菌灵及其混剂对柑橘青霉和绿霉病菌的室内毒力以及增效比率(SR),同时测定了茶皂素与噻菌灵混剂对沙糖橘采后青绿霉菌的防治效果及其对贮藏品质的影响。结果表明,茶皂素与噻菌灵配比为8∶2时对柑橘青霉和绿霉病菌具有显著的防治效果,SR分别为2.77和1.60,表现为显著增效作用。茶皂素与噻菌灵混剂(8∶2)在浓度为400mg.L-1时可有效抑制沙糖橘采后青绿霉病的发生,发病率仅为1.53%,显著低于对照的86.59%。混剂处理有效保持了沙糖橘的品质,延缓了果肉可溶性固形物、可滴定酸及抗坏血酸含量的下降。

关键词(KeyWords)： 沙糖橘;茶皂素;噻菌灵;柑橘青霉;柑橘绿霉;品质

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30871660);; 广东省科技计划项目(2008B050100045)

作者(Author): 郝卫宁;李辉;杨柳;耿鹏;胡美英;

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DOI:

参考文献(References)：

• [1]ISMAIL M,ZHANG J.Post-harvest citrus diseases and their control[J].Outlooks on Pest Management,2004,15:29-35.
• [2]PANG Xue-qun,CHEN Yan-ni,HUANG Xue-mei,WANG Yue-hua,HU Wei-rong,ZHANG Zhao-qi.Chilling Temperature en-hances quality reduction in postharvest Sha-tangju(Citrus reticulataBlanco)fruit[J].Acta Horticulturae Sinic,2008,35(4):509-514.庞学群,陈燕妮,黄雪梅,汪跃华,胡位荣,张昭其.冷害导致砂糖橘果实品质劣变[J].园艺学报,2008,35(4):509-514.
• [3]BUS V G,BONGERS A J,RISSE L A.Occurrence of Penicilliumdigitatum and P.italicum resistant to benomyl,thiabendazole,andimazalil on citrus fruit from different geographic origins[J].PlantDisease,1991,75:1098-1100.
• [4]ZHANG Wei,AI Qi-jun,WU Zhen-yu,WEI Yue-qin.Inhibitoryeffect of pyrolin on Alternaria alternata causing occurrence of blackspot in postharvest pear[J].Journal of Fruit Science,2008,25(5):755-759.张伟,艾启俊,吴振宇,魏月琴.鹿蹄草素对梨采后黑斑病病原菌的抑制作用[J].果树学报,2008,25(5):755-759.
• [5]HU Mei-jiao,GAO Zhao-yin,LI Min,YANG Feng-zhen,ZHENGFu-cong.Antifungal ability of 72 Chinese herbal acetone extracts a-gainst 9 pathogens of post-harvest diseases of banana and mangofruits[J].Journal of Fruit Science,2007,24(3):349-354.胡美姣,高兆银,李敏,杨凤珍,郑服丛.72种中草药提取物对香蕉、杧果果实采后病害病原菌的抑菌活性[J].果树学报,2007,24(3):349-354.
• [6]SHENG Shu-xiang,OU Xiao-ming,ZHANG Li,LEI Man-xiang.Study on joint Action of benalaxyl and teasaponion against BotrytisCinerea Pers[J].Pesticide Science and Administration,2000,21(5):32-34.盛书祥,欧晓明,张俐,雷满香.苯霜灵与茶皂素对黄瓜灰霉病菌的联合作用研究[J].农药科学与管理,2000,21(5):32-34.
• [7]WANG Xiao-yi,HUANG Bing-qiu.Preliminary examination ofbioactivities of teasaponion on seven plant pathogenic fungi[J].PlantProtection Technology and Extension,1998,18(6):34-35.王小艺,黄炳球.茶皂素对7种植物病原真菌的生物活性初测[J].植保技术与推广,1998,18(6):34-35.
• [8]HAO W N,ZHONG G H,HU M Y,LUO J J,WENG Q F,MUHAM-MAD R.Control of citrus postharvest green and blue mold and sourrot by tea saponin combined with imazalil and prochloraz[J].Posthar-vest Biology and Technology,2010,56:39-43.
• [9]HUANG Zhang-xin.Experimental guide of plant chemical protection[M].Beijing:China Agricultural Press,1993:52-58.黄彰欣.植物化学保护实验指导[M].北京:中国农业出版社,1993:52-58.
• [10]HAN Li-juan,GU Zhong-yan,WANG Qiang,HUANG Xiang-lin,XU Xiao-long.Mixture of pesticide[M].Nanjing:Jiangsu TechnologyPress,1994:31-51.韩丽娟,顾中言,王强,黄祥麟,许小龙.农药复配与复配农药[M].南京:江苏科学技术出版社,1994:31-51.
• [11]LI Jia-qing.Manual on keep fresh of fruits and vegetables[M].Bei-jing:China Light Industry Press,2003:104-128.李家庆.果蔬保鲜手册[M].北京:中国轻工业出版社,2003:104-128.
• [12]BOWYER P,CLARKE B R,LUNNESS P,DANIELS M J,OS-BOURN A E.Host range of a plant pathogenic fungus determined bya saponin detoxifying enzyme[J].Science,1995,267:371-374.
• [13]HE Xiao-ling,GAO Jin-yong,SHAO Jing-wei.Acute toxicity effecton earthworm,plutella xylostella and fusarium oxysporum by teasaponin[J].Journal of Fuzhou University,2007,35(5):785-788.何晓玲,高进勇,邵敬伟.茶皂素对蚯蚓、小菜蛾及枯萎病原真菌的急性毒性研究[J].福州大学学报,2007,35(5):785-788.
• [14]LV Qing,XU Zhi-de,XIA Jin-chu.Postharvest diseases of citrusand the fresh keep technology by chemicals[J].Hunan AgriculturalSciences,2000,4:44-45.吕庆,徐志德,夏金初.柑橘贮藏期病害及药剂防腐保鲜技术研究[J].湖南农业科学,2000,4:44-45.
• [15]GULLINO M L,KUIJPERS A M.Social and political implication ofmanaging plant diseases with restricted fungicides in Europe[J].An-nal of Review Phytopathology,1994,32:559-579.
• [16]DONG Yong,LI Yu-shan.Experiment research of tea saponin on theanti-inflammatory and analgesic actions[J].Chinese Journal of Clini-cal Rational Drug Use,2009,2(16):13-14.童勇,李玉山.茶皂素抗炎镇痛作用的实验研究[J].临床合理用药杂志,2009,2(16):13-14.
• [17]XU Shang-cheng.Progress and perspective in research and develop-ment of agrochemicals[J].Modern Agrochemicals,2002,1:7-13.徐尚成.农药研究开发的进展和展望[J].现代农药,2002,1:7-13.