新疆野苹果分离群体的构建和评价Construction and evaluation of the segregation population in Malus sieversii
刘遵春;苗卫东;刘大亮;陈学森;
摘要(Abstract)：
【目的】为了建立合适的用于构建新疆野苹果遗传连锁图镨的分离群体,【方法】以新疆红肉苹果和‘红富士’为材料,利用人工授粉方法构建了分离群体,采用SSR分子标记技术对作图亲本多态性、杂种纯度和群体内部的遗传结构进行了检测。【结果】结果表明,新疆红肉苹果和‘红富士’的遗传差异较大;排除了15株不确定的个体,确定110株作为新疆野苹果遗传图谱构建的作图群体;株系间无重复现象;64对SSR引物组合在作图亲本间共检测到232个多态性位点,其中有191个位点在P<0.01水平上符合孟德尔期望分离比,占多态性位点总数的82.30%。【结论】选取该群体作为构建新疆野苹果遗传连锁图镨的分离群体是合适的。
关键词(KeyWords)： 新疆野苹果;SSR;分离群体
基金项目(Foundation): 国家自然科学基金项目(31171932);; 山东省农业良种工程项目(鲁农良种字[2011]7号)
作者(Authors): 刘遵春;苗卫东;刘大亮;陈学森;
DOI: 10.13925/j.cnki.gsxb.2012.05.014
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