王帅;王海波;王孝娣;史祥宾;王宝亮;郑晓翠;刘凤之;

• 1:中国农业科学院果树研究所·农业部园艺作物种质资源利用重点实验室

摘要(Abstract)：

【目的】明确氨基酸硒和6-BA对葡萄叶片衰老与活性氧代谢的影响,为延缓叶片衰老技术的提出提供理论依据。【方法】以设施延迟栽培条件下叶片衰老速度不同的‘意大利’和‘无核白鸡心’2个葡萄品种为试材,分别进行叶面喷施氨基酸硒和6-BA处理,以喷施等量清水作为对照,研究施硒及6-BA对叶片衰老期间各功能叶片的叶绿素含量、净光合速率(Pn)、丙二醛(MDA)含量和超氧阴离子自由基(O2·)、过氧化氢(H2O2)含量及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)抗氧化酶活性的影响。【结果】与对照相比,外源氨基酸硒和6-BA处理显著延缓了叶片的叶绿素含量和净光合速率(Pn)的下降,提高了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性,从而减慢了丙二醛(MDA)、超氧阴离子自由基(O2·)、过氧化氢(H2O2)的上升速率,延缓了叶片衰老。2个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。【结论】氨基酸硒和6-BA处理可有效提高叶片的抗衰老能力,延长叶片的功能期。

关键词(KeyWords)： 葡萄;叶片衰老;6-BA;氨基酸硒;活性氧

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系建设专项资金项目(nycytx-30-zp);; 948重点项目“鲜食葡萄新品种及设施化生产技术引进与创新应用(2011-G28)”;; 国家自然科学基金“基于能值理论的我国北方地区葡萄设施栽培可持续性评价(41101573)”;; 国家外专局项目“国外葡萄优新品种示范基地建设及推广(Y20130326001)”

作者(Author): 王帅;王海波;王孝娣;史祥宾;王宝亮;郑晓翠;刘凤之;

Email:

DOI: 10.13925/j.cnki.gsxb.20140269

参考文献(References)：

• [1]WANG Haibo,WANG Xiaodi,WANG Baoliang,HE Jinxing,LIU Wanchun,LIU Fengzhi.Research advances in delayed viticulture of Grape[J].Sino-Overseas Grapevine&Wine,2008(1):47-51.王海波,王孝娣,王宝亮,何锦兴,刘万春,刘凤之.葡萄延迟栽培的研究进展[J].中外葡萄与葡萄酒,2008(1):47-51.
• [2]WANG Kaituo,ZHENG Yonghua,DI Huatao,MA Li,LU Xia,HUANG Ke.Effect of defense responses induced by BTH treatment on sucrose metabolism in grape cell suspensions[J].Journal of Fruit Science,2014,31(1):66-71.汪开拓,郑永华,狄华涛,马莉,卢霞,黄珂.BTH处理诱导葡萄悬浮细胞防卫反应对其蔗糖代谢的影响[J].果树学报,2014,31(1):66-71.
• [3]SMART C M.Gene expression during leaf senescence[J].New Phytologist,1994,126(3):419-448.
• [4]GAN S,AMASINO R M.Making sense of senescence(molecular genetic regulation and manipulation of leaf senescence)[J].Plant Physiology,1997,113:313-319.
• [5]MCCORD J M,FRIDOVICH I.Superoxide dismutase an enzymic function for erythrocuprein(hemocuprein)[J].Journal of Biological Chemistry,1969,244(22):6049-6055.
• [6]GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48(12):909-930.
• [7]TAN Yao,WANG Wenju,ZHANG Yahong.Effects of gibberellic acid on the senescence of grapevine leaves in autumn-prolonging cultivation[J].Sino-Overseas Grapevine&Wine,2007(4):18-20.谭瑶,王文举,张亚红.设施葡萄延后栽培中赤霉素处理对叶片衰老的影响[J].中外葡萄与葡萄酒,2007(4):18-20.
• [8]GHANEM M E,ALBCETE A,MARTINEZ-ANDUJAR C,ACOSTA M,ROMERO-ARANDA R,DODD I C,LUTTS S,PEREZ-Al FOCEA F.Hormonal changes during salinity-induced leaf senescence in tomato(Solanum lycopersicum L.)[J].Journal of Experimental Botany,2008,59(11):3039-3050.
• [9]LIM P O,WOO H R,NAM H G.Molecular genetics of leaf senescence in Arabidopsis[J].Trends in Plant Science,2003,8(6):272-278.
• [10]ZHENG Shasha,SUN Chuanfan,SUN Hongchun,LIU Liantao,ZHAO Jinfeng,LI Cundong.Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton[J].Scientia Agricultura Sinica,2009,42(12):4383-4389.郑莎莎,孙传范,孙红春,刘连涛,赵金峰,李存东.不同外源激素对花铃期棉花主茎叶生理特性的影响[J].中国农业科学,2009,42(12):4383-4389.
• [11]CAI Yongping,CEHN Jingxian.The effect of 6-BA and ABA on the senescence and the transport rate of assimilation production of flag leaf in rice[J].Journal of Anhui Agricultural Sciences,1996,24(2):113-115.蔡永萍,陈静娴.水稻抽穗期喷施6-BA,ABA对旗叶衰老及同化物运输的影响[J].安徽农业科学,1996,24(2):113-115.
• [12]MOUSSA H R,AHMED A E F M.Protective role of selenium on development and physiological responses of Vicia faba[J].International Journal of Vegetable Science,2010,16(2):174-183.
• [13]ZHAO Linchuan,YU Binggao.Regulation of selenium in the senescence of corn leaves[J].Journal of Nanjing Agricultural Univer-sity,1996,19(1):22-25.赵林川,俞炳杲.硒对玉米叶片衰老的调节作用[J].南京农业大学学报,1996,19(1):22-25.
• [14]DJANAGUIRAMAN M,DEVI D D,SHANKER A K,SHEEBA J A,BANGARUSAMY U.Impact of selenium spray on monocarpic senescence of soybean(Glycine Max L.)[J].Journal of Food Agriculture and Environment,2004,2:44-47.
• [15]ZOU Qi.Experimental guidance of plant physiology[M].Beijing:China Agriculture Press,2000:72-75.邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:72-75.
• [16]GIANNOPOLITIS C N,RIES S K.Superoxide dismutases I.Occurrence in higher plants[J].Plant Physiology,1977,59(2):309-314.
• [17]OMRAN R G.Peroxide levels and the activities of catalase,peroxidase,and indoleacetic acid oxidase during and after chilling cucumber seedlings[J].Plant Physiology,1980,65(2):407-408.
• [18]CAKMAK I,MARSCHNER H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J].Plant Physiology,1992,98(4):1222-1227.
• [19]ZHAO Shijie,XU Changcheng,ZOU Qi,MENG Qingwei.Improvement of method for measurement of malondialdehyde in plant tissues[J].Plant Physiology Communications,1994,30(3):207-210.赵世杰,许长成,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
• [20]WANG Aiguo,LUO Guanghua.Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants[J].Plant Physiology Communications,1990,26(6):55-57.王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,26(6):55-57.
• [21]PROCHAZKOVA D,SAIRAM R K,SRIVASTAVA G C,SINGH D V.Oxidative stress and antioxidant activity as the basis of senescence in maize leaves[J].Plant Science,2001,161(4):765-771.
• [22]BRENNAN T,FRENKEL C.Involvement of hydrogen peroxide in the regulation of senescence in pear[J].Plant Physiology,1977,59(3):411-416.
• [23]SAKURABA Y,SCHELBERT S,PARK S Y,HAN S H,LEE B D,ANDRES C B,KESSLER F,HORTENSTEINER S,PAEK N C.STAY-GREEN and chlorophyll catabolic enzymes interact at light-harvesting complex II for chlorophyll detoxification during leaf senescence in Arabidopsis[J].The Plant Cell Online,2012,24(2):507-518.
• [24]LU Q,LU C,ZHANG J,KUANG T G.Photosynthesis and chlorophyll a fluorescence during flag leaf senescence of field-grown wheat plants[J].Journal of Plant Physiology,2002,159(11):1173-1178.
• [25]LU Dingzhi,PAN Yucai,MA Yuefang,LIN Zongda,BAO Weixian,JIN Yimin,YOU Shupeng.Physiological and biochemical studies on leaf senescence at heading and grain formation stage in hybrid rice[J].Scientia Agricultura Sinica,1988,21(3):21-26.陆定志,潘裕才,马跃芳,林宗达,鲍为群,金逸民,游树鹏.杂交水稻抽穗结实期间叶片衰老的生理生化研究[J].中国农业科学,1988,21(3):21-26.
• [26]DOWENS B P,CROWELL D N.Cytokinin regulates the expression of a soybeanβ-expansin gene by a post-transcriptional mechanism[J].Plant Molecular Biology,1998,37(3):437-444.
• [27]GERM M,KREFT I,OSVALD J.Influence of UV-B exclusion and selenium treatment on photochemical efficiency of photosystem II,yield and respiratory potential in pumpkins(Cucurbita pepoL.)[J].Plant Physiology and Biochemistry,2005,43(5):445-448.
• [28]TEWARI R K,SINGH P K,WATANABE M.The spatial patterns of oxidative stress indicators co-locate with early signs of natural senescence in maize leaves[J].Acta Physiologiae Plantarum,2013,35(3):949-957.
• [29]DJANAGUIRAMAN M,DEVI D D,SHANKER A K,SHEEBA J A,BANGARUSAMY U.Selenium-an antioxidative protectant in soybean during senescence[J].Plant and Soil,2005,272(1-2):77-86.
• [30]STOPARIC G,MAKSIMOVIC I.The effect of cytokinins on the concentration of hydroxyl radicals and the intensity of lipid peroxidation in nitrogen deficient wheat[J].Cereal Research Communications,2008,36(4):601-609.
• [31]WU Yongyao,LU Xiangyang,PENG Zhenkun,LUO Zemin.Effect of Se on physiological and biochemical characters of Paddy Rice[J].Scientia Agricultura Sinica,2000,33(1):100-103.吴永尧,卢向阳,彭振坤,罗泽民.硒在水稻中的生理生化作用探讨[J].中国农业科学,2000,33(1):100-103.
• [32]PANDA D,SARKAR R K.Natural leaf senescence:probed by chlorophyll fluorescence,CO2photosynthetic rate and antioxidant enzyme activities during grain filling in different rice cultivars[J].Physiology and Molecular Biology of Plants,2013,19(1):43-51.
• [33]ZIMMERMANN P,ZRNTGRAF U.The correlation between oxidative stress and leaf senescence during plant development[J].Cellular and Molecular Biology Letters,2005,10(3):515-534.
• [34]LIU Qunlong,WANG Duo,WU Guoliang,HAO Guowei,HAO Yanyan,SUN Sheng.Effects of selenium on leaf senescence andantioxidase system in Pyrus bretschneide‘rDangshan Suli’[J].Acta Horticulturae Sinica,2011,38(11):2059-2066.刘群龙,王朵,吴国良,郝国伟,郝燕燕,孙胜.硒对酥梨叶片衰老及抗氧化酶系统的影响[J].园艺学报,2011,38(11):2059-2066.
• [35]ZHANG Haina,LI Cundong,XIAO Kai.Regulation effects of exogenous6-BA on photosynthesis and leaf senescence in cotton[J].Cotton Science,2008,19(6):467-471.张海娜,李存东,肖凯.外源6-BA对棉花光合和叶片衰老特性的调控效应研究[J].棉花学报,2008,19(6):467-471.
• [36]WANG Z,POTE J,HUANG B.Responses of cytokinins,antioxidant enzymes,and lipid peroxidation in shoots of creeping bentgrass to high root-zone temperatures[J].Journal of the American Society for Horticultural Science,2003,128(5):648-655.
• [37]ZHAO Liying,DENG Xiping,SHAN Lun.The response mechanism of active oxygen species remvoing system to drought stress[J].Acta Botan ica Boreali-Occidentalia Sinica,2005,25(2):413-418.赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005,25(2):413-418.
• [38]KAR M,MISHRA D.Catalase,ceroxidase,and polyphenoloxidase activities during rice leaf senescence[J].Plant Physiology,1976,57(2):315-319.
• [39]ARTHUR J R.The glutathione peroxidases[J].Cellular and Molecular Life Sciences,2001,57(13-14):1825-1835.
• [40]XUE T,HARTIKAINEN H,PIIRONEN V.Antioxidative and growth-promoting effect of selenium on senescing lettuce[J].Plant and Soil,2001,237(1):55-61.
• [41]BARNA B,SMIGOCKI A C,BAKER J C.Transgenic production of cytokinin suppresses bacterially induced hypersensitive response symptoms and increases antioxidative enzyme levels in Nicotiana spp[J].Phytopathology,2008,98(11):1242-1247.
• [42]GAN S,AMASINO R M.Inhibition of leaf senescence by autoregulated production of cytokinin[J].Science,1995,270(5244):1986-1988.