王帅;王海波;王孝娣;史祥宾;王宝亮;郑晓翠;刘凤之;

• 1:中国农业科学院果树研究所·农业部园艺作物种质资源利用重点实验室

摘要(Abstract)：

【目的】明确氨基酸硒和6-BA对葡萄叶片衰老与活性氧代谢的影响,为延缓叶片衰老技术的提出提供理论依据。【方法】以设施延迟栽培条件下叶片衰老速度不同的‘意大利’和‘无核白鸡心’2个葡萄品种为试材,分别进行叶面喷施氨基酸硒和6-BA处理,以喷施等量清水作为对照,研究施硒及6-BA对叶片衰老期间各功能叶片的叶绿素含量、净光合速率(Pn)、丙二醛(MDA)含量和超氧阴离子自由基(O2·)、过氧化氢(H2O2)含量及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)抗氧化酶活性的影响。【结果】与对照相比,外源氨基酸硒和6-BA处理显著延缓了叶片的叶绿素含量和净光合速率(Pn)的下降,提高了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性,从而减慢了丙二醛(MDA)、超氧阴离子自由基(O2·)、过氧化氢(H2O2)的上升速率,延缓了叶片衰老。2个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。【结论】氨基酸硒和6-BA处理可有效提高叶片的抗衰老能力,延长叶片的功能期。

关键词(KeyWords)： 葡萄;叶片衰老;6-BA;氨基酸硒;活性氧

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系建设专项资金项目(nycytx-30-zp);; 948重点项目“鲜食葡萄新品种及设施化生产技术引进与创新应用(2011-G28)”;; 国家自然科学基金“基于能值理论的我国北方地区葡萄设施栽培可持续性评价(41101573)”;; 国家外专局项目“国外葡萄优新品种示范基地建设及推广(Y20130326001)”

作者(Author): 王帅;王海波;王孝娣;史祥宾;王宝亮;郑晓翠;刘凤之;

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